Sources Sought Question and Answer.pdf

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Human Whole Genome Sequencing and Human Whole Genome Bisulfite Sequencing
Solicitation number
75N95021Q00173
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Sources Sought

Solicitation Number: 75N95021Q00173 Title: Human Whole Genome Sequencing and Human Whole Genome Bisulfite Sequencing

QUESTION:

1. Quick point of clarification on this sources sought for hWGS and hWGBS This is what is listed:

• For WGS, 90 G raw data per sample (30X coverage)

• For WGBS 300 G raw data per sample (100X coverage)

The coverage requirement for the bisulfite sequencing seems very high – do you truly want 300 Gb raw data per sample (100X coverage)? Typically, it’s around ~30X coverage. Just wanted to clarify. Thank you!

ANSWER: Yes, we wanted 100x coverage for the WGBS.

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