D_75N95019Q00295_Purchase_Description.pdf

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

PURCHASE DESCRIPTION

Statement of Need and Purpose: The purpose of this acquisition is to acquire one (1) MANTIS

Liquid Handler and accessories, or brand-name equal, for the Stem Cell Translation Laboratory

(SCTL) within the National Center for Advancing Translational Sciences (NCATS) Division of

Preclinical Innovation (DPI). The instrument will dedicate to molecular assays to deliver accurate and precise liquid handling in low volume. The MANTIS Liquid Handler provides a novel approach to reagent dispensing that provides researchers with an alternative to traditional air displacement pipetting. This instrument will be used by SCTL scientists for next generation sequencing (NGS), proteomics, and cell dosing studies.

Background Information and Objective: The opioid crisis highlights the urgent need for novel, non-addictive pain medications, as well as improved treatments for opioid addiction and overdose. There is a need for pharmacological agents directed at novel targets to test new therapeutic hypotheses, new clinic-ready drugs directed at those targets, and new testing systems with the potential to be more predictive of human clinical response than traditionally used models. Through the Helping to End Addiction Long-term (HEAL) Initiative, NCATS will focus on the development of human cell-based models of opioid misuse and addiction and of pain. The Stem Cell Translation Laboratory (SCTL) is a state-of-the-art research facility within

NCATS’ Division of Pre-Clinical Innovation dedicated to addressing the scientific and technological challenges in the iPSC field. Specifically, the SCTL aims to develop a renewable supply of human cell-based models focusing on new patient-derived iPSC lines that give rise to specialized, functional nerve cells that are relevant for opioid misuse and addiction, and pain.

Purchase Description: The MANTIS Liquid Handler is a programmable, non-contact liquid dispenser that uses positive displacement to maintain accuracy and precision. The MANTIS has a low dead volume (as little as 6 ul) and a minimum dispensing volume of 100 nL. The

MANTIS also includes continuous flow dispensing in deep well plates, two wash stations for sterilization and for final rinse, and built-in tube holders for large volume dispenses (e.g., for compound screening and for 96-well and 384-well plates).

In addition to the commonly-used, silicone-based chips for protein- or cell-based assay reagents, the molecular biology grade chips and the perfluoroelastomer-based chips are available for preparation of gene expression assays and compound plates in DMSO, respectively. When outfitted with a large capacity chip changer (LC3) the MANTIS can accommodate up to 24 chips, allowing for multiplexing of reagents. The carousel can be set up with either 15mL or 50mL falcon tubes.

The specific brand-name items are listed in the table below.

Solicitation No. 75N95019Q00295

Item

No.

Part No. Description Qty

1 MANTV3.3_RFID

Mantis V3.3 with Integrated RFID. Chip Changer - 6 Chip capacity and Mantis Controlling Software Instrument License

2 MCF Mantis Continuous Flow Option for MANTV3 1

3 MLC3R_RFID

One RFID LC3 Base for use on the right side of the Mantis.

Includes: One LC3, right side locating plate, waste station adapter, left side (4 chip) ACC, and 4 chip pallets when purchased with a Mantis.

4 MLC350

One 18 chip carousel. 50mL Falcon tube compatible.

Includes 18 pallets (does not include chips).

5 MCLVPR2

Low Volume 3PFE Mantis chip for maximum chemical compatibility, including RFID tag (0.1uL and 0.5uL).

Pack of two.

6 MCLVSR12

Low Volume Injection Molded Silicone Mantis Chip including

RFID tag (0.1uL and 0.5uL). Pack of 12.

7 MCHVSR12

High Volume Injection Molded Silicone Mantis Chip including

RFID tag (1uL and 5uL). Pack of 12.

8 MCHCF6

High Volume Mantis Continuous Flow Silicone chip for aqueous solutions. Pack of six.

9 MITLV10 Mantis LV chip tubing. 10 feet in length, clear, 0.016" ID. 1

10 MITHV10 Mantis HV chip tubing. 10 feet in length, green, 0.030" ID. 1

11 MCFSCF50.6

Mantis continuous flow septa cap for 50mL Falcon tube

(bottles not included). Pack of six.

12 232400 PCR Plate Adapter with wide conical pins for automated plate handling

13 230989 15 mL Falcon tube adapter 9

14 232396 Mantis 2mL Eppendorf Tube Adapter. Spring-loaded. 9

15 N/A

Optional Preventative Maintenance or Extended Warranty

(est. 9/2020 – 9/2024)

Covers hardware and software for the following items:

• Mantis V3.3 with Integrated RFID (Item No. 1)

• Mantis Continuous Flow Option for MANTV3 (Item No. 2)

• RFID LC3 Base (Item No. 3)

Salient Characteristics of the brand-name or equal liquid dispenser include:

1. Programmable, non-contact liquid dispenser

2. Utilizes positive displacement dispensing

3. Has minimum dispensing volume of 100 nL

4. Has dimensions no greater than 15 in. x 12 in. x 10 in. (W x H x D)

5. Has a dead volume of no more than 8 ul

6. Can accommodate at least 20 chips

7. Offers different chip materials for chemical compatibility

8. Comes with a warranty of at least one year

Delivery Date: Delivery to be made within twenty (20) weeks after receipt of order.