75N91024Q00125_SON.pdf

STATEMENT OF NEED (SON)

75N91024Q00125 Page 1 of 3 National Institutes of Health / National Cancer Institute / Office of Acquisitions

1.0 TITLE

Procurement of One (1) Brand Name or Equal Invitrogen Attune NxT Flow Cytometer w/ 4-Lasers (blue/red/violet6/yellow)

2.0 BACKGROUND

The Center for Immuno-Oncology (CIO), Center for Cancer Research (CCR), National Cancer Institute (NCI), National Institutes of Health (NIH) fosters high-risk basic discovery research in immunology and tumor biology. Through preclinical translational research, to paradigm-shifting clinical trials, CIO aims to develop novel immunotherapeutic treatments for human carcinomas. One aspect of CIO’s studies is identifying and quantifying the impact of various treatments on the phenotype of tumor cells using flow cytometry. Flow cytometric analysis allows to ascertain changes in abundance of proteins expressed by the tumor after treatment with various compounds, enabling to identify compounds which have the potential to enhance the sensitivity of tumor cells to lysis by tumor-reactive lymphocytes. CIO also measures the effects these compounds have on components of the immune system. Flow cytometric analysis allows CIO to ascertain any changes in populations of lymphocytes in circulation, primary/secondary lymphoid tissues, and within the tumor microenvironment after treatment with various modalities.

As the analysis of experiments becomes more sophisticated, CIO seeks a flow cytometer that enables rapid processing, reduces the amount of fluids required, and supports rare event analyses. Therefore, CIO requires one (1) Brand Name or Equal Invitrogen Attune NxT flow cytometer with 4-lasers (blue/red/violet6/yellow) and acoustic focusing technology.

3.0 TYPE OF ORDER

This shall be a Firm Fixed-Price Purchase Order.

4.0 SPECIAL ORDER REQUIREMENTS

ITEM DESCRIPTION QTY UNIT

1 Brand Name or Equal Invitrogen Attune NxT flow cytometer with 4-lasers (blue/red/violet6/yellow) and acoustic focusing technology (Model# A29004)

1 EA

2 Brand Name or Equal SmartStart 3-day on-site training

(TRN00180)

1 EA

NOTE: NCI will not be responsible for any shipping charges not listed in the quotation.

4.1 PRODUCT FEATURES/SALIENT CHARACTERISTICS

The following product features/characteristics are required for this requirement:

• Shall be a compact benchtop model, not to exceed 40 cm x 44 cm x 59 cm.

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• Shall be capable of a minimum rate of up to 35,000 events per second and electronic speed of up to 65,000 events per second.

• Shall have the ability to process samples at flow rates from 12.5 ul to up to 1 ml per minute, while maintaining a high quality of data collection.

• Shall be able to measure particles ranging between 0.5-50 um in size.

• Shall process sample volumes ranging between 20 ul and 4 ml.

• Shall allow the separation of white blood cells from red blood cells and platelets from intact whole blood, without the need to wash or lyse the cells, to minimize cell loss and shorten sample preparation.

• Shall be equipped with four (4) excitation lasers:

i. Blue (488 nm)

ii. Red (638 nm)

iii. Violet (405 nm)

iv. Yellow (561 nm)

• Shall have fourteen (14) fluorescent detection channels, which shall include the following filters: 450/40, 525/50, 610/20, 660/20, 710/50, 780/60, 530/30, 695/40, 585/15, 620/15, 780/60, 670/14, 720/30, and 780/60.

• Hardware shall include one (1) computer workstation (desktop and monitor) with Windows 10 (or higher) operating system.

• Accompanying software shall be intuitive and easy to use.

• The flow cytometer shall have the capacity to be upgraded with additional lasers and an autosampler.

4.2 WARRANTY

In addition to the Implied warranty contained in FAR Clause 52.212-4, the Contractor is encouraged to provide express warranties. At a minimum, the Contractor shall provide a 12-month warranty from date of final installation on all equipment and associated items provided under, or as a part of this purchase order.

The warranty shall include repair and or replacement of parts, including travel, labor, and materials, at no additional cost to the Government. The warranty shall not exclude or limit the Governments rights for an Implied warranty as established in the terms and conditions of clause 52.212-4.

4.3 DELIVERY / INSTALLATION

Delivery shall be within six (6) weeks of the purchase order award. All shipping/handling (including FOB Destination) and delivery/installation fees shall be included in the quote. Upon delivery, the Contractor shall notify the respective NCI Technical Point of Contact to schedule installation dates and times. Installation shall occur within fourteen (14) calendar days of delivery and shall be performed by, or under the direct supervision of, a certified operator.

The contractor shall deliver and install the equipment at the following address:

75N91024Q00125 Page 3 of 3

Center for Immuno-Oncology, National Institutes of Health/NCI 9000 Rockville Pike Bethesda, MD 20892 Technical Point of Contact: [to be provided upon award]

4.3.1 DELIVERY GUIDANCE

The Contractors shall communicate and follow instructions from the Technical Point of Contact (TPOC) to coordinate delivery and installation.

The Contractor shall direct all courier and delivery services – including those not operated by the Contractor (e.g. FedEx, USPS, UPS) – to follow the instructions in this section.

All companies delivering to the NIH main campus must comply with Commercial Vehicle Inspection Facility (CVIF) access procedures. Upon arrival, the CVIF will provide a list of buildings and loading docks to delivery personnel.

Deliveries will be logged in at the loading dock, and the loading dock contractor or Government personnel will attempt to contact the TPOC listed on the package. In the event that the TPOC is unable to accept the package, the delivery shall not be left at the loading dock station. The delivery must be returned to the Contractor, and the Contractor must coordinate with the TPOC to deliver on another date at no additional cost.

Deliveries can only be made between 6:00 a.m. and 4:00 p.m. EST, Monday – Friday, excluding federal holidays.

4.4 INSPECTION AND ACCEPTANCE

Pursuant to FAR Clause 52.212-4, all items to be delivered in relation to this Purchase Order are subject to final inspection and acceptance by an authorized representative of the Government. The authorized representative of the Government is the TPOC who is responsible for inspection and acceptance of all services, materials, or supplies to be provided by the Contractor.

4.5 TRAINING

The Contractor shall provide on-site training for up to 6 users. Training shall be conducted by a certified operator. The user training shall include an overview of setup approaches, instrument settings, and a demonstration of the performance. Instrument care and maintenance must also be covered and information about accessing unlimited technical support. The training shall be provided at a time specified by the Technical Point of Contact within 7 business days following installation of the device.

All training expenses, including materials and/or travel expenses, shall be included in the quoted price.

5.0 PAYMENT

Payment shall be made in full after the successful delivery, installation, training, and acceptance of equipment by the NCI Technical Point of Contact (TPOC). Payment authorization requires submission and approval of invoices to the NCI TPOC and NIH OFM.