Specifications.pdf

Specifications for: Electrolytic cell for S-S bond reduction

The Influenza group in the Clinical Chemistry Branch, Division of Laboratory Sciences, requires an electrolytic cell for sulfur-sulfur bond reduction that can used with our existing hardware and easily integrated into our current hydrogen deuterium exchange (HDX) mass spectrometry workflow. This electrolytic cell will be a replacement part for a non-functioning cell currently being used.

Specifications

1. Compatibility. The u-PrepCell SS (204.4304) is compatible with our existing ROXY™ potentiostat, a specialty hardware voltage controller and software package already in use and coupled to our existing Waters HDX integrated platform.

2. Updated cell design for high pressure. The cell has been demonstrated to withstand the high back pressures generated by the Waters back-pressure regulator (BPR) used by our current method. The increased back pressures provide higher digestion efficiency and greater sequence coverage.

3. Updated cell design for stability and longevity. The u-PrepCell SS has a new simplified electrode configuration able to provide fast and efficient reduction of sulfur-sulfur (S-S) bonds in proteins with high reproducibility over extended timescales typical of HDX batch runs.

4. Validated. The electrolytic cell has a demonstrated application to HDX analysis by mass spectrometry.

Companies that offer electrochemical flow cells:

Only Antec Scientific makes a cell that is directly compatible with our existing ROXY™ potentiostat. Additionally, only Antec Scientific has a demonstrated application of their electrolytic cell for reduction of sulfur-sulfur (S-S) bonds in proteins, and more specifically, the application towards hydrogen deuterium exchange analysis by mass spectrometry. Several vendors offer Antec products, they are listed below.

List of Potential Vendors:

1. Antec https://antecscientific.com/detectors-and-analyzers/reaction-cells/uprepcell List price: $ 9,605.00 Manufacturer Part number: 204.4304

2. Verulam Scientific https://www.verulamscientific.com/antec-scientific-electrochemical-detectors-for-hplc/prep-cell/

3. GSA Advantage https://www.gsaadvantage.gov/advantage/s/search.do?q=0:2prepcell&db=0&searchType=0 https://antecscientific.com/detectors-and-analyzers/reaction-cells/uprepcell https://antecscientific.com/detectors-and-analyzers/reaction-cells/uprepcell https://www.verulamscientific.com/antec-scientific-electrochemical-detectors-for-hplc/prep-cell/ https://www.verulamscientific.com/antec-scientific-electrochemical-detectors-for-hplc/prep-cell/