48-well microelectrode array plates.pdf

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

PURCHASE DESCRIPTION

1. Statement of Need and Purpose:

The purpose of this requisition is to procure 48-well Axion CytoView microelectrode array plates used in neurotoxicity screening assays for the Ionis ASO toxicity collaborative project.

2. Background Information and Objective:

The Therapeutic Development Branch (TBD) within the National Center for Advancing

Translational Sciences (NCATS) Division of Preclinical Innovation (DPI) requires Axion microelectrode array (MEA) plates to meet the needs of the Ionis ASO toxicity collaborative project.

An integral part of this project is developing assays for predictive neurotoxicity of antisense oligonucleotides (ASOs) to facilitate streamlining the safety approval process of ASO treatments for rare diseases. MEAs are used for recording electrical signals from neuronal networks and are composed of a grid of planar metal microelectrodes dispersed across a small surface, typically incorporated into flat bottom wells of “cell culture”-like plates. Neurons seeded and cultured directly onto MEAs plates allows for the measurement of changes in extracellular voltage as neurons spontaneously produce action potentials and generate network connections between cells. Neurotoxic MEA screening assays with ASOs permit the testing of both acute and chronic toxicity by measuring alterations in neuronal network signaling over the course of hours, days, and weeks after compound dosing.

MEAs have been successfully used by NCATS scientists to characterize neuronal toxicity by measuring changes in electrical signaling between neurons after exposure to

ASOs of different toxicities. 48-well MEA plates have a total of 16 electrodes per well, allowing for high resolution data. The “CytoView” version of these MEA plates also have transparent bottoms, allowing for additional toxicity parameters such as cell morphology that can also be assessed throughout the experiment. These Axion plates are required for use with pre-existing equipment (Axion Biosystem Maestro Pro MEA reader) for measuring the electrical activity and is analyzed with pre-existing Axion software.

3. Generic Name of Product:

Multi-well Microelectrode Array plates

4. Purchase Description:

Trade name: Transparent bottom 48-well MEA Plate

Brand name: CytoView MEA 48-White plate

Manufacture: Axion Biosystems

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

Model: CytoView MEA 48, transparent bottom white walls

Catalog: M768-tMEA-48W

5. Salient characteristics

- 48-well format

- Transparent well bottom allowing visualization of neurons throughout culture/experiment

- 16 recording electrodes/well allows for high resolution data

- Compatible with pre-existing Axion Biosystems “Maestro Pro multiwell microelectrode array and Impedance system” and associated Axion data analysis software suite

- Plates contain barcode identifiers for sample and data management.

6. Quantity:

(10) boxes CytoView MEA 48-White plate (5 plates/box)

7. Delivery Date:

Within 30 working days of date of award

8. Period of Performance:

NA

9. Option(s):

NA