4.03 YCGA Sole Source Redacted.pdf

Source Selection Information – See FAR 2.101 and 3.104

JUSTIFICATION FOR SOLE SOURCE (Simplified Acquisitions - MPT to <$250K)

Document number: 1301309578-N6659626PR00010

The supplies or services described herein are being solicited on a Sole Source basis and competition is precluded for reasons indicated below. There are no substitutes available for the supplies or services.

Check One:

Service:

Contractor: Yale University

Yale Center for Genome Analysis (YCGA) Contractor Address: 150 Munson Street, New Haven, CT 06511-8917 Contractor Lab Address: 300 Heffernan Dr., B36, West Haven, CT 06511 Contractor POC: Christopher Castaldi & Uloma Onuma Phone #: 203-737-3155 & 203-785-5905 Email: christopher.castaldi@yale.edu;

uloma.onuma@yale.edu; GCAT3@yale.edu Cage Code/UEI: 4B992 / FL6GV84CKN57

Supply:

Restricted to the following source. If a Brand Name manufacturer distributes via dealers, ALSO provide dealer information.)

Original Manufacturer Name:

Manufacturer:

Manufacturer POC:

Manufacturer Phone #:

Manufacturer Address:

Dealer/Rep:

Dealer/Rep Address Phone No.

Email:

Description of the supplies or services required, the estimated price/cost, and required delivery date.

NSMRL requires the following sequencing and gene expression analysis services to facilitate the investigation of potential biomarkers associated with behavioral responses indicative of cognitive fatigue. Results of this investigation will help inform interventions to improve warfighter health and performance during operational deployments.

Services include:

RNA-seq library prep Poly A 300 Units @ $120.00 (Total Price $36,000.00)

NovaSeq X Plus 25B 2x150 (25M reads/sample)

2.67857 Units @ $3,830.00 (Total Price $10,258.92)

Small Batch Analysis 1 Unit @ $1,117.00 (Total Price: $1,117.00)

Large Batch Analysis 3 Units @ $841.00 (Total Price: $2,523.00)

Estimated Total Cost: $49,898.92 Estimated Service Completion Date: 30 June 2026

In conclusion, the requested services represent the minimum requirements of the government.

Describe in detail why only this source can furnish the requirements to the exclusion of other brand name sources.

(what specific characteristics of the supplies or services limit the availability to a Brand Name Source (e.g., unique features, function of the item, etc.)?)

The requested Genome Analysis (RNA sequencing and gene expression) services are within Naval Submarine Medical Research Laboratory (NSMRL)’s research parameters and are specifically provided by Yale University only. NSMRL requires RNA-sequencing and gene expression-related analysis-based services in which NSMRL-collected data can be processed and evaluated in accordance with protocol specifications. These analyses and services provide data preparation and quality controls to generate sequence reads, read processing for quantifying gene expression over time, identification of significant changes in gene expression over time, and inferences of biological processes potentially related to such changes.

The company does not have any subsidiaries, partnerships, or distributors that manufacture, sell their services. Although other companies perform Genome Analysis within the open market, NSMRL must use Yale University, Center for Genome Analysis for their RNA extraction and sequencing experiments to analyze the data using the correct collaborations maintained by Yale University. To obtain correct, accurate, and complete data for research pertaining to cognitive fatigue risk prediction using genetic and epigenetic factors, this service is required. No other known company to date will be able to satisfy the government’s needs within our research parameters.

The supplies or services must be compatible in all aspects (form, fit and function) with existing systems presently installed/performing. Describe the equipment/function you have now and how the new item/service must coordinate, connect, or interface with the existing system.

The Yale Center for Genome Analysis (YCGA) provides high-quality data output and a high degree of flexibility, specificity, and granularity when conducting analyses as required by NSMRL protocols. YCGA has provided similar services and fulfilled study parameters (including those of the preliminary stages of the current effort) as required by previous NSMRL projects. Continuity of these services into current project efforts provide consistency across data and methodology.

Moreover, as evidenced by previous collaborations with YCGA, YCGA’s physical proximity to NSMRL facilitates transporting samples for analysis and minimizes the risk of sample degradation that provides researchers with higher quality data that is delivered on a timelier basis within project timelines. The requested services are needed for data acquisition and processing for the ongoing study. The requested RNA-sequencing/gene expression analysis services are within government approved statistical research parameters that are specifically provided only by Yale University.

These are "direct replacement" parts/components for existing equipment.

A patent, copyright or proprietary data limits competition.

Yale University Center for Genome Analysis (CAGE 4B992) is a large business and the incumbent vendor to previous contracts and studies. Contract N0018925PG031 was the initial Genome Analysis (RNA sequencing and gene expression) Service experiments for the current ongoing study. Yale University already has possession of government approved research data and protocols that support Naval Submarine Medical Research Laboratory (NSMRL)’s ongoing studies.

Other information to support a Sole Source Action:

Procuring services from a vendor other than Yale University Center for Genome Analysis will compromise and negatively skew the integrity of research data collected. No other known vendor to date can satisfy this Government need. This is a logical follow-on procurement to Contract N0018925PG031 in support of the NSMRL’s ongoing research.

I CERTIFY THAT THE INFORMATION PROVIDED ABOVE IS COMPLETE AND ACCURATE TO THE BEST OF

MY KNOWLEDGE. I UNDERSTAND THAT THE PROCESSING OF THIS SOLE SOURCE JUSTIFICATION

PRECLUDES THE USE OF FULL AND OPEN COMPETITION.

Signature Date

Activity Title

I HEREBY DETERMINE THAT THE CIRCUMSTANCES OF THE CONTRACT ACTION DEEM ONLY ONE

SOURCE REASONABLY AVAILABLE IN ACCORDANCE WITH FAR 13.106-1(b)(1)(i).

Contracting Officer Signature Date

NAVSUP Fleet Logistics Center, Norfolk Contracting Department, Regional Directorate, Groton Office, Code 231.2