Attachment A - Flow Cytometry.pdf
PDF 187 KB Posted
- Attached to
- Clement J. Zablocki VAMC Flow Cytometry Testing Federal contract opportunity
- Solicitation number
- 36C25226Q01020002
About this file
Attachment A details estimated yearly test volumes and current 10-color Leukemia/Lymphoma panel configurations for laboratory testing. The estimated yearly test volumes include 1,100 T-Cell Subset tests, 250 Lymphocyte Subset tests, and various 10-Color Leukemia/Lymphoma panels: 650 Screen Panels, 320 B-Cell Panels, 85 Acute Panels, 85 Plasma Cell Panels, 75 T-Cell Panels, and 5 Hairy Cell Panels. The document provides comprehensive panel configurations, specifying the fluorochrome markers (FITC, PE, ECD, PC5.5, PC7, APC, AA700, AA750, Pac Blu, KrO) and corresponding antibody markers for each panel type across multiple tube configurations, including Screen, B-Cell, T-Cell, Hairy Cell, Plasma Cell, Acute, Cyto B-Cell, Cyto T-Cell, BAL T-Cell, Viability, and Verify Tube panels.
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| File | Type | Posted |
|---|---|---|
| 36C25226Q0102 0002.pdf |
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Text version
Attachment A – Estimated Test Volumes & Current Leukemia/Lymphoma Panels
Estimated Yearly Test Volumes Panel Estimated Yearly Volume
T-Cell Subsets (CD4/CD8) 1100
Lymphocyte Subsets (TBNK) 250
10-Color Leukemia/Lymphoma Panels Estimated Yearly Volume
Screen Panels 650
B-Cell Panels 320
Acute Panels 85
Plasma Cell Panels 85
T-Cell Panels 75
Hairy Cell Panels 5
Current 10-Color Leukemia/Lymphoma Panels
Screen
FITC PE ECD PC5.5 PC7 APC AA700 AA750 Pac Blu KrO
Tube 1 IgG1 IgG1 IgG1 IgG1 IgG1 IgG1 IgG1 IgG1 IgG1 CD45
Tube 2 Kappa Lambda CD19 CD4 CD38 CD5 CD34 CD3 CD8 CD45
B-Cell Panel
FITC PE ECD PC5.5 PC7 APC AA700 AA750 Pac Blu KrO
Tube 1 Kappa Lambda CD19 CD5 CD11c CD23 CD10 CD25 CD20 CD45
Tube 2 CD20 CD79b CD19 CD5 CD22 CD81 CD10 CD200 FMC7 CD45
T-Cell Panel
Tube 1 CD1a CD26 CD45RO TCR g/d CD7 TCRa/b CD4 CD3 CD5 CD8
Tube 2 CD57 CD30 CD2 CD4 CD7 CD16 CD56 CD3 CD5 CD8
Hairy Cell
Tube 1 Kappa Lambda CD19 X CD11c CD103 X CD25 CD20 CD45
Plasma Cell
Tube 1 cy IgG1 cy IgG1 IgG1 CD138 IgG1 IgG1 X CD38 IgG1 CD45
Tube 2 cyKappa cyLambda CD19 CD138 CD56 CD81 X CD38 CD20 CD45
Acute Panel
Tube 1 IgG1 IgG1 IgG1 IgG1 IgG1 IgG1 IgG1 IgG1 IgG1 CD45
Tube 2 CD15 CD64 CD14 CD13 CD11b CD117 CD34 CD33 HLA-DR CD45
Tube 3 CD7 CD10 CD19 CD3 CD22 CD117 CD34 CD38 CD20 CD45
Tube 4 cy IgG1 cy IgG1 X cy IgG1 cy IgG1 X CD34 X X CD45
Tube 5 cyTdT cyMPO X cyCD3 cy CD22 CD117 CD34 X X CD45
Cyto B-Cell
Tube 1 cy IgG1 cy IgG1 IgG1 IgG1 X X IgG1 IgG1 IgG1 CD45
Tube 2 cy Kappa cyLambda CD19 CD5 X X CD10 X CD20 CD45
Cyto T-Cell
Tube 1 IgG1 X IgG1 IgG1 IgG1 X X cyIgG1 IgG1 CD45
Tube 2 CD8 X CD19 CD4 CD7 X X cyCD3 CD5 CD45
BAL T-Cell
Tube 1 IgG1 X X IgG1 X X X IgG1 X CD45
Tube 2 CD8 X X CD4 X X X CD3 X CD45
Viability
Tube 1 CD45 X X 7AAD X X X X X X
Verify Tube
Tube 1 CD20 CD4 CD19 CD5 CD7 CD16 CD56 CD3 CD8 CD45
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