2269561 - J.A..pdf

Justification for Other than Full and Open Competition Brand Name at or Below the Simplified Acquisition Threshold

Project Title: Reagents for Spatial Transcriptomic of Human Norovirus

Identification of the agency and contracting activity

DHHS, NIH, NIAID, VRC

Vaccine Research Center 40 Convent Drive, Bldg. 40, Room 3614, Bethesda, MD 20892

Background and Justification

A. Project Background

Vaccine Research Center (VRC) mission is to discover and develop novel vaccines and biologics targeting infectious diseases of global public health importance. VRC combines a scientific and technological strategy innovation to achieve the design, development, manufacturer and evaluation of vaccines and therapeutics. Tissue Analysis Core

(TAC) contributes to VRC mission by characterizing immune signatures at tissue level to better understand natural infection and strategies of intervention and vaccines.

B. Project Description

To date little is known about host genes required for HNoV infection, cell tropism, immune signatures and immune evasion. Spatial transcriptomic has been determined to be the best approach to profile the cellular heterogeneity of tissues like stomach and intestine during HnoV infection. These supplies will be used in support of those research studies.

C. Description of the supplies to be purchased:

Product Code Product Quantity

1000644 Xenium Standalone Custom 301 to 480 Gene Panel (16 rxns) - Design ID# 4F6C74

1000665 Xenium Advanced Panel Upgrade (301 to 480 genes) -

Design ID# 4F6C74

1000644 Xenium Standalone Custom 301 to 480 Gene Panel (16 rxns) - Design ID# R33UDZ

1000665 Xenium Advanced Panel Upgrade (301 to 480 genes) -

Design ID# R33UDZ

1000662 Xenium Cell Segmentation Add-on Kit (2 rxns) 8

1000487 Xenium Decoding Consumables (1 run, 2 slides) 8

1000461 Xenium Decoding Reagents (1 run, 2 slides) 8

1000460 Xenium Slides & Sample Prep Reagents (2 slides, 2 rxns) 8

Name of manufacturer, producer, or distributor of each brand name product referenced:

10X Genomics

6230 Stoneridge Mall Road

Pleasanton, CA 94588-3260

Statutory authority permitting other than full and open competition

☒ 41 USC 253(c)(1) and FAR Part 6.302-1: Brand Name Requirement

Demonstration that the proposed brand name product’s salient characteristics (specific features, qualities and/or the nature of the acquisition) requires use of the authority cited above:

10xGenomics (Manufacturer) offers the required proprietary microfluidic circuits that rapidly partition input samples (e.g. higher molecular weight DNA, single cells), which are then barcoded using the GemCode Technology.

This technology is not manufactured by any other vendor than 10X Genomics is not available for any other instrumentation. The requested reagents meet our need to sequence thousands of transcriptomes in a single run from single cells and while obtaining phenotypic data from those same cells. The latter is performed by CITE-seq, which has only been tested on the 10X platform; changing the platform would require about a year of work, be costly and results could not be adequately compared to data from previous studies.

Requirements for the reagent kits:

• Simultaneously measure cell surface protein expression with gene expression

• Simultaneously measure TCR, and 3’ gene expression in the same cells

• Be able to detect protein of low abundance transcripts with increased phenotypic specificity

• Allow cell phenoptyping with hashtag technology that should also allow combining different donor samples

• Allow subcellular gene signature technology probe-based

• The kit must be compatible with the controller

Determination by the Contracting Officer that the anticipated cost/price to the Government will be fair and reasonable.

Certification This is to certify that the justification for the proposed acquisition has been reviewed and that to the best of my knowledge and belief the information and/or data provided to support my request (for the requestor) and approval (for the CO) of is accurate and complete.

Official Name & Title Signature Date

Contracting Officer Approval

Addison Miller Contracting Officer

9/11/2025