22-004516 - Marmoset Requisition Purchase Desciption.pdf

PURCHASE DESCRIPTION

1. Statement of Need and Purpose:

The purpose of this requirement is to expand the genetic diversity of NIH’s current marmoset breeding colony.

a) Expand the genetic diversity of our current marmoset breeding colony by inducing highbred vigor and concurrently meet the evolving research requirements of our program, by introducing twenty (20) breeding pairs of marmosets with known pedigree lines spanning four or more generations [Twenty (20) Breeder Females & Twenty (20)

Breeder Males].

b) Provide eight (8) Research Marmosets, without charge, to be identified by NIH to support its continued research.

c) Provide forty-eight (48) transportation crates for international marmoset transport. All animals to be shipped one per crate.

d) Provide for the transportation of forty-eight (48) transportation crates of marmosets from Monash University to the nearest international airport.

e) Provide for the preparation of forty-eight (48) marmosets for shipment by providing veterinary and technician services, as well as AEC and administrative support.

2. Background Information and Objective:

Our ability to modify the genome of various species has led to the development of new animal models to study human disease. These models have advanced our understanding of a disease’s origin, its progression, and possible intervention strategies. Rodents have been the species of choice for these models, secondary to their availability, ease of manipulation, and defined genetics. Over the past decade, a few institutions, including the

NIMH, have made advancements in developing a transgenic primate model using the marmoset. The anatomy and physiology of the marmoset are closer to that of humans than rodents. In addition, they also display similar complex behaviors in social groups, making them an ideal model for many human neurophysiology and behavioral studies.

Therefore, over the past decade, the NIMH DIR has invested time and resources in developing a transgenic marmoset research and breeding program to support the requirements of several investigators.

3. Generic Name of Product:

Common marmosets (Callithrix jacchus) are also called white-tufted marmosets or white-tufted-ear marmosets.

4. Purchase Description:

• Common marmosets (Callithrix jacchus) also called white-tufted marmoset or white-tufted-ear marmosets: Twenty (20) Established Breeding Pairs (40 animals); Eight (8) research common marmosets to be identified by Dr. James Bourne to be provided without cost.

• Production Of Forty-Eight (48) Transport Crates: No Charge

• Transportation To International Airport

• Pre-Transport Preparation of Animals including:

o Veterinary Services o Technician Services o Administrative Services

5. Salient Characteristics:

Provide forty (40) common marmosets (20 male and 20 female) for use as breeding animals, aged between 18 months and 7 years.

• Animals must be genetically distinct from animals already held for breeding at NIMH.

o Pedigree information going back to at least the great-grandparents of the forty (40) breeders being purchased shall be provided.

• Females must not be on pregnant for shipping.

The remaining eight (8) research marmosets shall be identified by NIH.

All animals shall have a health record demonstrating a minimum of three (3) consecutive negative intradermal tuberculin tests, or an industry excepted equivalent test, over the lifetime of the animal or colony health records demonstrating that the source colony is free of Mycobacterium tuberculosis for the past 3 years.

All animals must receive an intradermal tuberculin skin test or an industry equivalent test no sooner than ten (10) calendar days prior to shipping and this test must be negative.

All animals shall be negative for common pathogenic helminth and protozoal parasites on fecal exam performed within 30 days of delivery.

All animals must have no visible evidence of external parasites (e.g., lice, ticks, mites, ringworm, etc.).

All animals shall be in good health to include well-fleshed and in good physical condition and be free of clinical signs of disease (i.e., cardiovascular, respiratory, intestinal, internal/external parasitism, no history of chronic diarrhea (lasting longer than 2 weeks), inflammatory bowel disease, wasting syndrome, etc.).

All animals shall exhibit no obvious behavioral problems (the animal does not exhibit any stereotypic maladaptive behavior such as self-clasping, self-biting, or consistent circling and there is no physical evidence such as bite wounds or alopecia secondary to excessive self-grooming, that indicates that the animal is exhibiting these behaviors).

All animals shall have a veterinary exam indicating each animal is healthy enough to withstand the rigors of international transport shall be performed within 10 days of shipment.

All animals shall be between 180-600 grams body weight, with a body condition score of

2.5-4.5 with 1 begin emaciated and 5 being obese.

All animals shall be negative for Salmonella, Shigella on two (2) rectal cultures performed two (2) days in a row within 30 days prior to delivery.

Additionally, all animals shall be tested one (1) time via rectal culture and found negative for Klebsiella pneumoniae, hypermucoviscous phenotype, within 30 days prior to delivery.

Following rectal swab culture, the lab can conduct a string test on any Klebsiella pneumoniae strains identified (hypermucoviscous would be string test positive).

Detailed medical records for all animals shall be provided electronically for review and approval prior to shipment of the animals.

6. Quantity:

• Common marmosets (Callithrix jacchus) also called white-tufted marmoset or white-tufted-ear marmosets: Twenty (20) Established Breeding Pairs (40 animals); Eight (8) research common marmosets to be identified by Dr. James Bourne to be provided without cost.

• Production Of Forty-Eight (48) Transport Crates

• Transportation To International Airport

• Pre-Transport Preparation of Animals including:

o Veterinary Services o Technician Services o Administrative Services

7. Delivery Date:

NIH has an urgent need for these animals to meet the established timelines for current ongoing research programs. We understand that obtaining the required international permits for the shipment of the animals is estimated to take several months.

8. Period of Performance:

Not pplicable.

9. Option(s):

None