2 - RFQ Attachment brand name or equal specifications.pdf

RFQ Attachment – RFQ-NIAID-2087197 Brand-name or Equal

Brand Specifications: (Quantity of 1 of each of items below)

Item Description Catalog No.

1 Leica EM ACE600 230 V / 50 60 Hz, 115 V / 60 Hz 16771527 2 3 axis (rotation, height, tilt) motorized stage 16771537 3 For uniform coatings of highly fissured specimen surfaces. 6 planets with pin holes (ø 3,2mm) capable of holding a variety of SEM Stubs

16771541

4 Foil Target Cr, DN 54 x 1 mm, 99.96%, 7.2 g/cm3 16771566 5 Foil Target Ir DN 54 x 0.3 mm, 99.99%, 22.56 g/cm3 16771560 6 Service Installation on the sputtering coater 9I_EM_CLASS_E 7 Leica EM GP2 plunge freezer 16706402 8 Viewing System 16706447 9 Service Installation of the plunge freezer 9I_EM_CLASS_D

To be considered for award, vendors of “equal” products, including “equal” products of the brand-named manufacturer, must meet the criteria for the salient characteristics that follow:

GP2 grid plunge freezer:

• Must have one sided blotting and possibility to blot grid from the rear.

• Must have a single blot sensor.

• Must have temperature and humidity control of the environmental chamber.

• Must have a control of the secondary cryogen (liquid nitrogen) temperature for stable primary cryogen (Ethane) temp.

• Must have a microscope viewing system for quality monitoring of specimen during blot and freeze.

• Must have a liquefying head for filling of secondary cryogen to prevent spillover contamination into the primary cryogen.

• Must be installed on-site

Sputter Coater:

• Must be able to protect beam-sensitive specimens.

• Must be a two-source concept with angled ports and a rotating stage allowing for an evenly distributed thin film over a 100mm stage. This resulting thin layer of metal should allow for high magnification SEM analysis up to 200kX and beyond.

• Must be able to facilitate sample preparation of room temp and cryo samples.

• Configurable with a dedicated low angle rotary shadowing (LARS) setup to image proteins or DNA strands by TEM.

• Must double as a carbon evaporator allowing the preparation of a thin but strong support layer for TEM grids.

• Must be compatible with EM VCT500 transfer system, to support cryo-EM workflow by providing an interface for cryo transfer coating under cryo conditions and allowing a freeze-fracture capability.

• Must be installed on-site