2_JOFOC 2068434.docx

Justification for Other than Full and Open Competition at or Below the Simplified Acquisition Threshold

Project Title: Miltenyi Biotec gentleMACS Octo Dissociator with Heaters

I. Identification of the agency and contracting activity

DHHS, NIH, NIAID, IRF-0CS

II. Description of the project, and supplies or services required to meet the agency’s needs A. Project Background The gentleMACS tissue dissociator will be used to generate high quality single cell suspensions in support of our 10X Genomics sc-RNAseq capability. The gentleMACS is a key part of the 10X sc-RNAseq workflow, and listed by both Illumina and 10X Genomics as a required part of the verified workflow. The machine will also be used by Immunology to prepare tissues for a variety of Immunological assays in support of IRF studies.

The gentleMACS tissue dissociator will be used to generate high quality single cell suspensions in support of sequencing our SARS-CoV-2 stocks and experimental samples.

B. Project Description The Miltenyi Biotec gentleMACS Octo Dissociator with Heaters is a benchtop instrument used for the automated dissociation or homogenization of up to eight samples simultaneously. scRNA-seq continues to be a leading analytical platform that allows for the ability to further define our understanding of the transcriptomic profile of individual cells. This allows for an increased level of clarity in the diversity of transcripts detected within a given sample that traditional total RNA sequencing cannot provide. The gentleMACS is an integral part of the 10X Genomics single cell RNA sequencing pipeline. A critical step in scRNAseq is the recovery of fully dissociated, intact, and viable individual cells. Sample clumping, cellular debris, and dead cells have a significant deletrious effect on the quality of a scRNA-seq run and increase costs. Miltenyi Biotec, 10X Genomics and Illumina partnered to produce a streamlined workflow to facilitate high quality scRNA-seq runs. The purchase of the gentleMACS allows the IRF to continue to expand and optimize our sequencing capabilities with a verified system. The machine will also be used in the preparation of single cell suspensions for a variety of immunological assays and methods used in support of IRF studies.

III. Identification of the statutory authority permitting other than full and open competition ☒ 41 USC 253(c)(1) and FAR Part 6.302-1: Only one responsible source

IV. Demonstration that the proposed contractor(s) unique qualifications or the nature of the acquisition requires use of the authority cited.

A. Name and address of the proposed contractor(s) Miltenyi Biotec Inc.

2303 Lindbergh St Auburn, CA 95602 Ph: 1-530-888-8871

B. Describe why only the requested supply/service will meet the government’s needs The gentleMACS is an integral part of the 10X Genomics single cell RNA sequencing pipeline. A critical step in scRNAseq is the recovery of fully dissociated, intact, and viable individual cells. Sample clumping, cellular debris, and dead cells have a significant deletrious effect on the quality of a scRNA-seq run and increase costs. Miltenyi Biotec, 10X Genomics and Illumina partnered to produce a streamlined workflow to facilitate high quality scRNA-seq runs. The purchase of the gentleMACS allows the IRF to continue to expand and optimize our sequencing capabilities with a verified system. The machine will also be used in the preparation of single cell suspensions for a variety of immunological assays and methods used in support of IRF studies.

C. Describe why only the proposed contractor is capable of providing the requested supply/service Miltenyi Biotec, 10X Genomics and Illumina partnered to produce a streamlined workflow to facilitate high quality scRNA-seq runs. The purchase of the gentleMACS allows the IRF to continue to expand and optimize our sequencing capabilities with a verified system.