2_JA 2129923 KD Medical.pdf

Justification for Other than Full and Open Competition at or Below the Simplified Acquisition Threshold

Project Title: Single-cell technologies for Gene Expression Analysis of Parasites and Vectors in LMVR

I . Identification of the agency and contracting activity

DHHS, NIH, NIAID, DIR, LMVR, MCIU

Address of the requesting lab/office: 12735 Twinbrook Parkway, Rockville, MD 20852

II. Description of the project, and supplies or services required to meet the agency’s needs

A. Project Background:

The culture of malaria parasites is an ongoing activity of the LMVR. Tissue culture media, Sodium Bicarbonate and gentamycin will be used by researchers in the Malaria Culture and Insectary Unit of the Laboratory of Malaria and Vector Research for the ongoing experiments in the Insectary involving invitro feeding of malaria parasites to mosquitoes.

B. Project Description;

Line Description,Catalog # 1 KD Gentamicin 10mg/ml CAG-1240 2 KD7.5% Sodium Bicarbonate RGC 5120 3 RPMI 1640 with L-Glutamine+25mMHepes+50ug./ml.Hypoxanthine CUS-0645

III. Identification of the statutory authority permitting other than full and open competition 41USC 1901(a)(1) and 13.106-1(b): Only one responsible source/Brand Name

IV. Demonstration that the proposed contractor unique qualifications or the nature of the acquisition requires use of the authority cited.

A. Name and address of the proposed contractor

K-D Medical 6935-G Oakland Mills, Rd Columbia, MD 21045-4719

B. Describe why only the requested supply/service will meet the government’s needs:

KD Medical has provided the exact formulation of the media that is necessary to grow human red blood cells that are capable of hosting a malarial infection reproducibly. It is essential to the function of the cell culture unit of LMVR. The specific media is required for continuity of results and assay systems used in experiments leading up to the current ongoing malaria research.