2_JA 2107514 10X Genomics LISB.docx

Justification for Other than Full and Open Competition at or Below the Simplified Acquisition Threshold

Project Title: Acquisition of 10X Genomics Kits I. Identification of the agency and contracting activity

II. Description of the project, and supplies or services required to meet the agency’s needs (including the estimated value) A. Project Background:

The LISB is studying the systems biology of the immune system on a global scale, and at single cell resolution. The 10x Genomics single cell platform allows profiling of tens of thousands of single cells in parallel to to explore the diversity of innate and adaptive immune system with multiomic readouts and flexibity to scale. The platform allows profiling of gene expression at high resolution in an easy to use platform, consisting of the 10x Chromium Controller hardware, and the companion kits of reagents and consumables that allow use of the system.

Project Description:

These reagents will be used on an existing NIH-owned 10x Chromium X Controller to examine antigen specificity with 5’ gene expression of millions of blood immune cells from adult healthy donor as well as vaccinated donors. These data will allow the dissection of cell types, cell states, and gene-gene networks that underly immune responses in health and disease.

III. Identification of the statutory authority permitting other than full and open competition (SELECT ONE) ☒ 41 USC 253(c)(1) and FAR Part 6.302-1: Only one responsible source

IV. Demonstration that the proposed contractor(s) unique qualifications or the nature of the acquisition requires use of the authority cited.

A. Name and address of the proposed contractor(s):

10X Genomics, Inc.

7068 Koll Center Parkway Suite 401 Pleasanton, California 94566 United States

B. Describe why only the requested supply/service will meet the government’s needs:

The Chromium kits and reagents are specific to the 10x Chromium X Controller available for our use; no other kits/reagents are compatible with this instrument, and attempting to run third party reagents would not be supported by the instrument manufacturer.

C. Describe why only the proposed contractor is capable of providing the requested supply/service:

The 10x Chromium X controller system requires proprietary reagents and consummables that can only be provide by the manufacterer, 10x Genomics.