B08_Attachment_1_-_Specifications_0001.xlsx
XLSX spreadsheet 23 KB Posted
- Attached to
- HAZARDOUS WASTE DISPOSAL
- Solicitation number
- 140G0226Q0182
About this file
This is a hazardous waste specifications attachment detailing the composition and disposal requirements for chemical waste streams generated at the Halawa Necropsy Facility. The document provides a comprehensive inventory of 16 distinct waste categories, each with detailed chemical compositions, container specifications, and associated CAS numbers for hazardous constituents.
The waste streams include: DAB substrate (staining waste containing methanol and nickel compounds in 400mL quantities); five DNA extraction waste types (DNA-1 through DNA-5) containing guanidine hydrochloride, ethanol, and various buffer solutions in 100mL containers); decalcification waste (containing hydrogen chloride); five Trumps fixative batches (containing paraformaldehyde and glutaraldehyde in volumes ranging from 150mL to 500mL); EDTA decalcification waste (4-liter containers); Cal Ex II decalcification waste (24 containers of 4-liters each, containing formaldehyde and formic acid); and Z-fix fixative waste (3 containers of 4-liters each, containing formaldehyde and ionized zinc). All waste streams are liquid-based and packaged in plastic bottles of varying sizes. The specifications include manufacturer information (Fisher Scientific, Qiagen, Electron Microscopy Sciences, Sigma, Anatech) and detailed chemical compositions with percentage ranges, enabling proper hazardous waste classification, handling, transportation, and disposal by the contracted waste management vendor.
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Other files
| File | Type | Posted |
|---|---|---|
| Sol_140G0226Q0182_Amd_0001.pdf | ||
| B03__Attachment_2_-_DOL_Wage_Determination_0001.pdf | ||
| Sol_140G0226Q0182.pdf |
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Text version
Sheet1
| Ref | Description of waste | Waste Components | # of Containers | Container Size | Amount of Waste | Container Type | Solid/Liquid/Both | Manufacturer | Cat # | Cas # | Process that generated waste | Location |
| DAB-1 | DAB substrate diluted 1:10 in stable peroxidase buffer | Methanol (3.5-5%), Diethylenetriaminepentaacetic acid (01-0.5%), nickel compounds (0.05-0.1%), ethylene glycol (0.7-2.5%), cobalt II chloride hexahydrate (0.05-0.1%), 3,3' diaminobenzidine (0.01-0.1%) | 1 | 500ml plastic bottle | 400mL | plastic bottle | Liquid | Thermofisher | 34065 | 67-56-1, 67-43-6, 7718-54-9, 107-21-1, 7791-13-1, 91-95-2 | Staining western blot membranes | Halawa Necropsy Facility |
| DNA-1 | DNA waste | DNeasyPlantMiniKit: P3- Acetic acid (64-19-7) 10-20%; Buffer AP1- Sodium dodecyl sulphate (151-21-3) 1-10%; Buffer AW2- diluted in 100% Ethanol (when reconstituted); Buffer AW1- Guanidine hydrochloride (50-01-1) 50-70% diluted in 100% ethanol when reconstituted. DNeasyBlood&TissueKit: ProteinaseK- (39450-01-6), 1-10%; Buffer AL- Guanidine hydrochloride (50-01-1) 30-50%; Buffer AW2- diluted in 100% Ethanol (when reconstituted); Buffer AW1- Guanidine hydrochloride (50-01-1) 50-70% diluted in 100% ethanol when reconstituted. maleic acid (110-16-7) 0.1-1%. QIAquickGelExtractionKit: Buffer QG- Guanidinium thiocyanate (593-84-0) 50-70%; cresol red (1733-12-6) 1-10%. | 1 | Pee jar | 100ml | plastic bottle | Liquid | Qiagen | 69104, 69504, 28704 | 64-19-7, 151-21-3, 50-01-1, 39450-01-6, 110-16-7, 593-84-0, 1733-12-6 | DNA and gel extraction | Halawa Necropsy Facility |
| DNA-2 | DNA waste | DNeasyPlantMiniKit: P3- Acetic acid (64-19-7) 10-20%; Buffer AP1- Sodium dodecyl sulphate (151-21-3) 1-10%; Buffer AW2- diluted in 100% Ethanol (when reconstituted); Buffer AW1- Guanidine hydrochloride (50-01-1) 50-70% diluted in 100% ethanol when reconstituted. DNeasyBlood&TissueKit: ProteinaseK- (39450-01-6), 1-10%; Buffer AL- Guanidine hydrochloride (50-01-1) 30-50%; maleic acid (110-16-7) 0.1-1%; Buffer AW2- diluted in 100% Ethanol (when reconstituted); Buffer AW1- Guanidine hydrochloride (50-01-1) 50-70% diluted in 100% ethanol when reconstituted. | 1 | Pee jar | 100ml | plastic bottle | Liquid | Qiagen | 69104, 69504 | 64-19-7, 151-21-3, 50-01-1, 39450-01-6, 110-16-7 | DNA extraction | Halawa Necropsy Facility |
| DNA-3 | DNA waste | DNeasyBlood&TissueKit: ProteinaseK- (39450-01-6), 1-10%; Buffer AL- Guanidine hydrochloride (50-01-1) 30-50%; maleic acid (110-16-7) 0.1-1%; Buffer AW2- diluted in 100% Ethanol (when reconstituted) 64-17-5; Buffer AW1- Guanidine hydrochloride (50-01-1) 50-70% diluted in 100% ethanol when reconstituted. QIAampDNA Micro kit: ProteinaseK- (39450-01-6), 1-10%; glycerol- (56-81-5), 70-90%; Buffer ATL- Sodium dodecyl sulphate (151-21-3), 1-10%; Buffer AL- Guanidine hydrochloride (50-01-1) 30-50%; maleic acid (110-16-7) 0.1-1%; Buffer AW2- diluted in 100% Ethanol (when reconstituted); Buffer AW1- Guanidine hydrochloride (50-01-1) 50-70% diluted in 100% ethanol when reconstituted. | 1 | Pee jar | 100ml | plastic bottle | Liquid | Qiagen | 69506 (BloodTissue), 56304 (QiaAMP) | 39450-01-6, 50-01-1, 110-16-7, 56-81-5, 151-21-3, 110-16-7, 64-17-5. | DNa extraction | Halawa Necropsy Facility |
| DNA-4 | DNA waste | DNeasyBlood&TissueKit: ProteinaseK- (39450-01-6), 1-10%; Buffer AL- Guanidine hydrochloride (50-01-1) 30-50%; maleic acid (110-16-7) 0.1-1%; Buffer AW2- diluted in 100% Ethanol (when reconstituted); Buffer AW1- Guanidine hydrochloride (50-01-1) 50-70% diluted in 100% ethanol when reconstituted (64-17-5). | 1 | Pee jar | 100ml | plastic bottle | Liquid | Qiagen | 69506 (BloodTissue) | 39450-01-6, 50-01-1, 110-16-7, 64-17-5. | DNa extraction | Halawa Necropsy Facility |
| DNA-5 | DNA waste | DNeasyBlood&TissueKit: ProteinaseK- (39450-01-6), 1-10%; Buffer AL- Guanidine hydrochloride (50-01-1) 30-50%; maleic acid (110-16-7) 0.1-1%; Buffer AW2- diluted in 100% Ethanol (when reconstituted); Buffer AW1- Guanidine hydrochloride (50-01-1) 50-70% diluted in 100% ethanol when reconstituted. QIAampDNA Micro kit: ProteinaseK- (39450-01-6), 1-10%; glycerol- (56-81-5), 70-90%; Buffer ATL- Sodium dodecyl sulphate (151-21-3), 1-10%; Buffer AL- Guanidine hydrochloride (50-01-1) 30-50%; maleic acid (110-16-7) 0.1-1%; Buffer AW2- diluted in 100% Ethanol (when reconstituted); Buffer AW1- Guanidine hydrochloride (50-01-1) 50-70% diluted in 100% ethanol when reconstituted. DNeasyPlantMiniKit: P3- Acetic acid (64-19-7) 10-20%; Buffer AP1- Sodium dodecyl sulphate (151-21-3) 1-10%; Buffer AW2- diluted in 100% Ethanol (when reconstituted); Buffer AW1- Guanidine hydrochloride (50-01-1) 50-70% diluted in 100% ethanol when reconstituted. Allprep PowerViral DNA/RNA kit: Solution IRS- Ammonium acetate AAS-12 (not assigned)- 1-10%; Solution PM3- guanidine hydrochloride (50-01-1), 70-90%, t-Octylphenoxypolyethoxyethanol Triton X-100 (9002-93-1), 10-20%; Solution PM4- Ethanol (64-17-5), 90-100%; Solution PM5- Isopropanol (67-63-0), 50-70%; Solution PM1- Guanidinium thiocyanate (593-84-0), 1-10%; | 1 | Pee jar | 100ml | plastic bottle | Liquid | Qiagen | 69506 (BloodTissue), 56304 (QiaAMP), 69104 (Plant), 28000-50 (Allprep) | 39450-01-6, 50-01-1, 64-17-5, 56-81-5, 151-21-3, 110-16-7, 64-19-7, 9002-93-1, 67-63-0, 593-84-0. | DNa extraction | Halawa Necropsy Facility |
| DECAL-1 | Decal Stat | Hydrogen chloride, Acid mists | 1 | Quart | Quart | plastic bottle | Liquid | Fisher Scientific | STL121232 | 7647-01-0, RR-129844-8 | Fish decalcification for histology | Halawa Necropsy Facility |
| Trumps 1 | Trumps fixative | Paraformaldehyde (30525-89-4) 16%;Glutaraldehyde (111-30-8) 2.5%. | 1 | 250ml | 250mL | plastic bottle | Liquid | Electron Microscopy Sciences; Sigma | 15710, 5882 | 30525-89-4, 111-30-8 | Electron microscopy fixative | Halawa Necropsy Facility |
| Trumps 2 | Trumps fixative | Paraformaldehyde (30525-89-4) 16%;Glutaraldehyde (111-30-8) 2.5%. | 1 | 250ml | 250mL | plastic bottle | Liquid | Electron Microscopy Sciences; Sigma | 15710, 5882 | 30525-89-4, 111-30-8 | Electron microscopy fixative | Halawa Necropsy Facility |
| Trumps 3 | Trumps fixative | Paraformaldehyde (30525-89-4) 16%;Glutaraldehyde (111-30-8) 2.5%. | 1 | 250ml | 250mL | plastic bottle | Liquid | Electron Microscopy Sciences; Sigma | 15710, 5882 | 30525-89-4, 111-30-8 | Electron microscopy fixative | Halawa Necropsy Facility |
| Trumps 4 | Trumps fixative | Paraformaldehyde (30525-89-4) 16%;Glutaraldehyde (111-30-8) 2.5%. | 1 | 150mL | 150mL | plastic bottle | Liquid | Electron Microscopy Sciences; Sigma | 15710, 5882 | 30525-89-4, 111-30-8 | Electron microscopy fixative | Halawa Necropsy Facility |
| Trumps 5 | Trumps fixative | Paraformaldehyde (30525-89-4) 16%;Glutaraldehyde (111-30-8) 2.5%. | 1 | 500mL | 500mL | plastic bottle | Liquid | Electron Microscopy Sciences; Sigma | 15710, 5882 | 30525-89-4, 111-30-8 | Electron microscopy fixative | Halawa Necropsy Facility |
| E | EDTA Waste | EDTA, NaOH, | 1 | 4L/gallon | 4L / Gallon | plastic bottle | Liquid | Fisher Scientific | O2793, S318 | 6381-92-6, 1310-73-2, 497-19-8 | Slow decalcifiction for coral histology | Halawa Necropsy Facility |
| C | Cal Ex II waste | Formaldehyde, formic acid, methyl alcohol, water | 24 | 4L / Gallon | 4L / Gallon | plastic bottle | Liquid | Fisher Scientific | CS511-4D | 50-00-0, 67-56-1, 7732-18-5 | Coral decalcification for histology | Halawa Necropsy Facility |
| Z | Z-fix waste | Formaldehyde, ionized zinc, methanol, water | 3 | 4L / Gallon | 4L / Gallon | plastic bottle | Liquid | Anatech via Fisher Scientific | NC9050753 | 50-00-0, 67-56-1 | Coral fixation for histology | Halawa Necropsy Facility |
https://www.fishersci.com/shop/products/cal-ex-ii-fixative-decalcifier-fisher-chemical-2/CS5114D
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