SWFHU_-_Estimate_of_Testing_Numbers_0001.xlsx
XLSX spreadsheet 27 KB Posted
- Attached to
- NM DEXTER FISH AQUATIC ANIMAL DISEASE TESTING
- Solicitation number
- 140FS226Q0165
About this file
This is an Estimate of Testing Numbers spreadsheet for a five-year Blanket Purchase Agreement (BPA) supporting the New Mexico Dexter Fish Health Unit's aquatic animal disease testing services. The document outlines monthly testing projections across three fiscal years (2026, 2027, and 2028) for inspection services, diagnostic studies, and consultation activities related to fish pathogen detection and analysis.
The testing scope encompasses viral diagnostics including cell culture testing on three cell lines (EPC, CHSE, and BF-2 cells) for viruses such as IPNV, IHNV, and VHSV, with projected annual volumes of 784 pooled tissue samples. Bacteriological inspections focus on detection of A. salmonicida and Y. ruckeri (55 annual inspections) and Flavobacteria species (60 annual inspections), with supporting antibiotic sensitivity testing (20 annual tests). Additional specialized testing includes Myxobolus cerebralis detection via pepsin/trypsin digest (252 annual samples) and PCR confirmation (6 annual tests), R. salmoninarum detection through FAT testing (836 annual samples) and ELISA testing on adult and juvenile salmon, and PCR-based testing for Ceratonova shasta, Nucleospora, WSIV, and other pathogens (80 annual tests). Diagnostic studies encompass histopathology services (20 cases with 1-3 fish, 6 additional fish analyses annually) and diagnostic virology, with consultation services and quality/SOP modifications available to accommodate operational needs (88 and 27 annual hours respectively). Testing volumes remain consistent across all three years, suggesting stable disease surveillance requirements under this five-year federal contract vehicle administered by the Department of the Interior Fish and Wildlife Service.
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Other files
| File | Type | Posted |
|---|---|---|
| Sol_140FS226Q0165_Amd_0001.pdf | ||
| PWS_for_Aquatic_Animal_Disease_Testing_Dexter.pdf | ||
| Sol_140FS226Q0165.pdf |
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| 3.1.1 | Inspection Services | Jul-26 | Aug-26 | Sep-26 | Oct-26 | Nov-26 | Dec-26 | Jan-27 | Feb-27 | Mar-27 | Apr-27 | May-27 | Jun-27 | TOTAL |
| A | Test samples by cell culture on 3 cell lines (EPC, CHSE and BF-2 cells) for viruses inc. IPNV, IHNV, and VHSV. Tissues are pooled. | 72 | 36 | 120 | 48 | 100 | 120 | 72 | 120 | 96 | 784 | |||
| A | Identification/confirmation of viruses isolated in culture | 2 | 2 | 2 | 3 | - 0 | - 0 | 2 | 2 | 2 | 4 | 1 | 1 | 21 |
| C,D | Bacteriology: Inspection for A. salmonicida and Y. ruckeri | 5 | 5 | 5 | 5 | - 0 | - 0 | 5 | 6 | 6 | 8 | 5 | 5 | 55 |
| C,D | Bacteriology: Inspection for Flavobacteria (coldwater, columnaris) | 10 | 10 | 10 | 10 | 10 | 10 | 60 | ||||||
| C | Antibiotic sensitivity testing for isolates detected in culture & Inspection for A. salmonicida and Y. ruckeri |
Hagen, Kathleen: Hagen, Kathleen:
Follow up test
| 1 | 2 | 1 | 1 | - 0 | - 0 | 1 | 2 | 4 | 3 | 3 | 2 | 20 | ||
| C | Custom Aquatic Antimicrobial Suspecptibility Panel (new test) | 2 | 1 | 1 | 1 | - 0 | - 0 | 1 | 1 | 1 | 1 | 1 | 1 | 11 |
| B | Test the samples for Myxobolus cerebralis by pepsin/trypsin digest | 72 | 108 | 72 | 252 | |||||||||
| B | Test the samples for Myxobolus cerebralis PCR confirmation (split 3.1.1 F into digest & PCR confirmation) | 2 | 2 | 2 | 6 | |||||||||
| G | Culture testing for R. salmoninarum |
Hagen, Kathleen: Hagen, Kathleen:
| Follow up test | - 0 | ||
| G.1 | Antibiotic sensitivity testing for R. salmoninarum |
Hagen, Kathleen: Hagen, Kathleen:
| Follow up test | - 0 | ||
| C | FAT testing of organ prints for R. salmoninarum by FAT |
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| Doubled for Fpsy | - 0 | - 0 | 200 | 200 | 200 | 200 | 36 | 836 | |||||||
| I | Perform ELISA tests from samples from adult and juvenile salmon for the dectection of R. salmoninarum | - 0 | |||||||||||||
| J | Test samples for Ceratonova shasta, Nucleospora, WSIV, Bd (amphibians), or other pathogens by PCR | 45 | 35 | 80 | |||||||||||
| 3.1.1 | Diagnostic Studies | - 0 | |||||||||||||
| A | Diagnostic virology on up to 4 cell lines (per case) | - 0 | |||||||||||||
| A | Bacteriology | - 0 | |||||||||||||
| A | Bacteriology antibiotic sensitivity | 1 | 2 | 1 | 2 | - 0 | - 0 | 1 | 4 | 5 | 5 | 4 | 25 | ||
| C | Histopathology (1-3 fish per case) | 1 | 1 | 2 | 3 | - 0 | - 0 | 1 | 2 | 2 | 3 | 3 | 2 | 20 | |
| C | Histopathology (each additional fish above 3) | 4 | 1 | 1 | 6 | ||||||||||
| 3.1.3 | Consultation | - 0 | |||||||||||||
| A | Consultation by phone (per hour) | - 0 | |||||||||||||
| B | Consultation by email (per hour) | - 0 | |||||||||||||
| C | Additional labor to ensure flexibility to meet the special operational needs | 10 | 10 | 10 | 5 | - 0 | - 0 | 3 | 10 | 10 | 10 | 10 | 10 | 88 | |
| D | Quality and SOP modifications to accommodate high # of positives | 3 | 3 | 3 | 2 | - 0 | - 0 | 1 | 3 | 3 | 3 | 3 | 3 | 27 |
Doubled for Fpsy
| 3.1.1 | Inspection Services | Jul-20 | Aug-20 | Sep-20 | Oct-20 | Nov-20 | Dec-20 | Jan-21 | Feb-21 | Mar-21 | Apr-21 | May-21 | Jun-21 | TOTAL |
| A | Test samples by cell culture on 3 cell lines (EPC, CHSE and BF-2 cells) for viruses inc. IPNV, IHNV, and VHSV. Tissues are pooled. | 72 | 36 | 120 | 48 | 100 | 120 | 72 | 120 | 96 | 784 | |||
| A | Identification/confirmation of viruses isolated in culture | 2 | 2 | 2 | 3 | - 0 | - 0 | 2 | 2 | 2 | 4 | 1 | 1 | 21 |
| C,D | Bacteriology: Inspection for A. salmonicida and Y. ruckeri | 5 | 5 | 5 | 5 | - 0 | - 0 | 5 | 6 | 6 | 8 | 5 | 5 | 55 |
| C,D | Bacteriology: Inspection for Flavobacteria (coldwater, columnaris) | 10 | 10 | 10 | 10 | 10 | 10 | 60 | ||||||
| C | Antibiotic sensitivity testing for isolates detected in culture & Inspection for A. salmonicida and Y. ruckeri |
Hagen, Kathleen: Hagen, Kathleen:
Follow up test
| 1 | 2 | 1 | 1 | - 0 | - 0 | 1 | 2 | 4 | 3 | 3 | 2 | 20 | ||
| C | Custom Aquatic Antimicrobial Suspecptibility Panel (new test) | 2 | 1 | 1 | 1 | - 0 | - 0 | 1 | 1 | 1 | 1 | 1 | 1 | 11 |
| B | Test the samples for Myxobolus cerebralis by pepsin/trypsin digest | 72 | 108 | 72 | 252 | |||||||||
| B | Test the samples for Myxobolus cerebralis PCR confirmation (split 3.1.1 F into digest & PCR confirmation) | 2 | 2 | 2 | 6 | |||||||||
| G | Culture testing for R. salmoninarum |
Hagen, Kathleen: Hagen, Kathleen:
| Follow up test | - 0 | ||
| G.1 | Antibiotic sensitivity testing for R. salmoninarum |
Hagen, Kathleen: Hagen, Kathleen:
| Follow up test | - 0 | ||||||||||||||
| C | FAT testing of organ prints for R. salmoninarum by FAT | - 0 | - 0 | 200 | 200 | 200 | 200 | 36 | 836 | ||||||
| I | Perform ELISA tests from samples from adult and juvenile salmon for the dectection of R. salmoninarum | - 0 | |||||||||||||
| J | Test samples for Ceratonova shasta, Nucleospora, WSIV, Bd (amphibians), or other pathogens by PCR | 45 | 35 | 80 | |||||||||||
| 3.1.1 | Diagnostic Studies | - 0 | |||||||||||||
| A | Diagnostic virology on up to 4 cell lines (per case) | - 0 | |||||||||||||
| A | Bacteriology | - 0 | |||||||||||||
| A | Bacteriology antibiotic sensitivity | 1 | 2 | 1 | 2 | - 0 | - 0 | 1 | 4 | 5 | 5 | 4 | 25 | ||
| C | Histopathology (1-3 fish per case) | 1 | 1 | 2 | 3 | - 0 | - 0 | 1 | 2 | 2 | 3 | 3 | 2 | 20 | |
| C | Histopathology (each additional fish above 3) | 4 | 1 | 1 | 6 | ||||||||||
| 3.1.3 | Consultation | - 0 | |||||||||||||
| A | Consultation by phone (per hour) | - 0 | |||||||||||||
| B | Consultation by email (per hour) | - 0 | |||||||||||||
| C | Additional labor to ensure flexibility to meet the special operational needs | 10 | 10 | 10 | 5 | - 0 | - 0 | 3 | 10 | 10 | 10 | 10 | 10 | 88 | |
| D | Quality and SOP modifications to accommodate high # of positives | 3 | 3 | 3 | 2 | - 0 | - 0 | 1 | 3 | 3 | 3 | 3 | 3 | 27 |
| 3.1.1 | Inspection Services | Jul-21 | Aug-21 | Sep-21 | Oct-21 | Nov-21 | Dec-21 | Jan-22 | Feb-22 | Mar-22 | Apr-22 | May-22 | Jun-22 | TOTAL |
| A | Test samples by cell culture on 3 cell lines (EPC, CHSE and BF-2 cells) for viruses inc. IPNV, IHNV, and VHSV. Tissues are pooled. | 72 | 36 | 120 | 48 | 100 | 120 | 72 | 120 | 96 | 784 | |||
| A | Identification/confirmation of viruses isolated in culture | 2 | 2 | 2 | 3 | - 0 | - 0 | 2 | 2 | 2 | 4 | 1 | 1 | 21 |
| C,D | Bacteriology: Inspection for A. salmonicida and Y. ruckeri | 5 | 5 | 5 | 5 | - 0 | - 0 | 5 | 6 | 6 | 8 | 5 | 5 | 55 |
| C,D | Bacteriology: Inspection for Flavobacteria (coldwater, columnaris) | 10 | 10 | 10 | 10 | 10 | 10 | 60 | ||||||
| C | Antibiotic sensitivity testing for isolates detected in culture & Inspection for A. salmonicida and Y. ruckeri |
Hagen, Kathleen: Hagen, Kathleen:
Follow up test
| 1 | 2 | 1 | 1 | - 0 | - 0 | 1 | 2 | 4 | 3 | 3 | 2 | 20 | ||
| C | Custom Aquatic Antimicrobial Suspecptibility Panel (new test) | 2 | 1 | 1 | 1 | - 0 | - 0 | 1 | 1 | 1 | 1 | 1 | 1 | 11 |
| B | Test the samples for Myxobolus cerebralis by pepsin/trypsin digest | 72 | 108 | 72 | 252 | |||||||||
| B | Test the samples for Myxobolus cerebralis PCR confirmation (split 3.1.1 F into digest & PCR confirmation) | 2 | 2 | 2 | 6 | |||||||||
| G | Culture testing for R. salmoninarum |
Hagen, Kathleen: Hagen, Kathleen:
| Follow up test | - 0 | ||
| G.1 | Antibiotic sensitivity testing for R. salmoninarum |
Hagen, Kathleen: Hagen, Kathleen:
| Follow up test | - 0 | ||||||||||||||
| C | FAT testing of organ prints for R. salmoninarum by FAT | - 0 | - 0 | 200 | 200 | 200 | 200 | 36 | 836 | ||||||
| I | Perform ELISA tests from samples from adult and juvenile salmon for the dectection of R. salmoninarum | - 0 | |||||||||||||
| J | Test samples for Ceratonova shasta, Nucleospora, WSIV, Bd (amphibians), or other pathogens by PCR | 45 | 35 | 80 | |||||||||||
| 3.1.1 | Diagnostic Studies | - 0 | |||||||||||||
| A | Diagnostic virology on up to 4 cell lines (per case) | - 0 | |||||||||||||
| A | Bacteriology | - 0 | |||||||||||||
| A | Bacteriology antibiotic sensitivity | 1 | 2 | 1 | 2 | - 0 | - 0 | 1 | 4 | 5 | 5 | 4 | 25 | ||
| C | Histopathology (1-3 fish per case) | 1 | 1 | 2 | 3 | - 0 | - 0 | 1 | 2 | 2 | 3 | 3 | 2 | 20 | |
| C | Histopathology (each additional fish above 3) | 4 | 1 | 1 | 6 | ||||||||||
| 3.1.3 | Consultation | - 0 | |||||||||||||
| A | Consultation by phone (per hour) | - 0 | |||||||||||||
| B | Consultation by email (per hour) | - 0 | |||||||||||||
| C | Additional labor to ensure flexibility to meet the special operational needs | 10 | 10 | 10 | 5 | - 0 | - 0 | 3 | 10 | 10 | 10 | 10 | 10 | 88 | |
| D | Quality and SOP modifications to accommodate high # of positives | 3 | 3 | 3 | 2 | - 0 | - 0 | 1 | 3 | 3 | 3 | 3 | 3 | 27 |
File details come from the government source that posted it. Updated .