B08_-_ATT_2_-_Fish_Disease_Testing_Price_Schedule_Feb_2023_v2_0002.xlsx
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- OR ARD-FSHRS FSH DISEASES TSTNG SRVCS
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- 140F0S23Q0007
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Pricing sheet
| Schedule of supplies and services | Price per test | ||||
| The FWS will require that the partner laboratory conduct testing for the Service (described below in 3.1.1) under either ISO 17025 or American Association of Veterinary Laboratory Diagnosticians (AAVLD) accreditation. Accreditation is necessary to insure the reliability of test results, to meet evolving regulatory and agency requirements, and to align with a nationwide effort by the FWS to do all aquatic animal disease testing in accredited laboratories. For the services below, refer to the USFWS Performance Work Statement. | 7/3/2023 - 7/2/2024 | 7/3/2024 - 7/2/2025 | 7/3/2025 - 7/2/2026 | 7/3/2026 - 7/2/2027 | 7/3/2027 - 7/2/2028 |
| Aquatic Aerobic Culture, Streptococcus iniae/Lactococcus garvieae | |||||
| Aquatic Aerobic Culture, Aeromonas salmonicida and Yersinia ruckeri (USFWS) | |||||
| Aquatic Diagnostic Viral Culture | |||||
| Aquatic Viral Culture (2 cell lines) | |||||
| Aquatic Viral Culture (3 cell lines) | |||||
| Flavobacterium psychrophilum Aerobic Culture | |||||
| Myxobolus cerebralis Pepsin Trypsin Digest | |||||
| Renibacterium salmoninarum (BKD) ELISA | |||||
| Renibacterium salmoninarum (BKD) FA | |||||
| Direct PCR and sequencing (bacteria, viruses, parasites, fungi) | |||||
| Molecular confirmation of virus isolates | |||||
| Aquatic antimicrobial Susceptibility test (MIC) | |||||
| Molecular detection of other pathogens (Chytrid (Bd, Bsal), Fpsy) | |||||
| Aquatic Histopathology (1-3 fish per case) | |||||
| Archiving bacterial or Viral isolates (-80C bacterial, LN2 or equivilent for viruses | |||||
| Shipping archived isolates to other partners |
Historical number of tests
| Annual Number of Tests | ||||
| 3.1.1 | Inspection Services | Year 2 (July 2019 - March2020) | Year 3 (July 2020 -March 2021) | Year 4 (July 2021-March 2022) |
| A | Test samples by cell culture on 2 cell lines (typically EPC and CHSE cells) for viruses inc. IPNV, IHNV, and VHSV. Tissues are pooled. | 1395 | 947 | 1383 |
| B | Identification/confirmation of viruses isolated in culture | |||
| C | Bacteriology: Inspection for A. salmonicida and Y. ruckeri | 2596 | 1395 | 2365 |
| D | Bacteriology: Inspection for Flavobacteria (coldwater, columnaris) | 1380 | 796 | 825 |
| E | Antibiotic sensitivity testing for isolates detected in culture & Inspection for A. solmonicida and Y. ruckeri |
Hagen, Kathleen: Hagen, Kathleen:
Follow up test
| 2 | 0 | |||
| E.1 | Custom Aquatic Antimicrobial Suspecptibility Panel (new test) | 4 | 58 | |
| F | Test the samples for Myxobolus cerebralis by pepsin/trypsin digest | 510 | 133 | 244 |
| F.1 | Test the samples for Myxobolus cerebralis PCR confirmation (split 3.1.1 F into digest & PCR confirmation) | 2 | 6 | |
| G | Culture testing for R. salmoninarum |
Hagen, Kathleen: Hagen, Kathleen:
Follow up test G.1 Antibiotic sensitivity testing for R. salmoninarum Hagen, Kathleen: Hagen, Kathleen:
Follow up test
| H | FAT testing of organ prints for R. salmoninarum by FAT | 386 | 480 | 595 |
| I | Perform ELISA tests from samples from adult and juvenile salmon for the dectection of R. salmoninarum | 4898 | 5511 | 4164 |
| J | Test samples for Ceratonova shasta, Nucleospora, WSIV, Bd (amphibians), or other pathogens by PCR | 56 | 2 | 30 |
| 3.1.2 | Diagnostic Studies | |||
| A | Diagnostic virology on up to 4 cell lines (per case) | 29 | 16 | 36 |
| B | Bacteriology | 0 | ||
| B.1 | Bacteriology antibiotic sensitivity | 0 | ||
| C | Histopathology (1-3 fish per case) | 42 | 30 | 42 |
| C.1 | Histopathology (each additional fish above 3) | 28 | 27 | 69 |
| 3.1.3 | Consultation | |||
| A | Consultation by phone (per hour) | |||
| B | Consultation by email (per hour) | |||
| C | Additional labor to ensure flexibility to meet the special operational needs | 1440 | 1440 | 1440 |
| D | Quality and SOP modifications to accommodate high # of positives | 360 | 360 | 360 |
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