Sol_140F0S23Q0001_Amd_0003.pdf

(x)

140F0S23Q0001 x x

1 copies of the amendment; (b) By acknowledging receipt of this amendment on each copy of the offer submitted ; or (c) By separate letter or electronic communication which includes a reference to the solicitation and amendment numbers. FAILURE OF YOUR ACKNOWLEDGEMENT TO BE

RECEIVED AT THE PLACE DESIGNATED FOR THE RECEIPT OF OFFERS PRIOR TO THE HOUR AND DATE SPECIFIED MAY RESULT IN REJECTION OF YOUR

OFFER. If by virtue of this amendment you desire to change an offer already submitted , such change may be made by letter or electronic communication, provided each letter or electronic communication makes reference to the solicitation and this amendment, and is received prior to the opening hour and date specified.

x

F0S

Falls Church VA 22041 5275 Leesburg Pike FWS, Over SAT G/S FWS Over SAT G/S

11/10/20220003

13. THIS ITEM ONLY APPLIES TO MODIFICATION OF CONTRACTS/ORDERS. IT MODIFIES THE CONTRACT/ORDER NO. AS DESCRIBED IN ITEM 14.

12. ACCOUNTING AND APPROPRIATION DATA (If required) is not extended.is extended, Items 8 and 15, and returning

Offers must acknowledge receipt of this amendment prior to the hour and date specified in the solicitation or as amended , by one of the following methods: (a) By completing

The above numbered solicitation is amended as set forth in Item 14. The hour and date specified for receipt of Offers

11. THIS ITEM ONLY APPLIES TO AMENDMENTS OF SOLICITATIONS

FACILITY CODE CODE

10B. DATED (SEE ITEM 13)

10A. MODIFICATION OF CONTRACT/ORDER NO.

9B. DATED (SEE ITEM 11)

9A. AMENDMENT OF SOLICITATION NO.

CODE

8. NAME AND ADDRESS OF CONTRACTOR (No., street, county, State and ZIP Code)

7. ADMINISTERED BY (If other than Item 6)CODE 6. ISSUED BY

PAGE OF PAGES

4. REQUISITION/PURCHASE REQ. NO.3. EFFECTIVE DATE2. AMENDMENT/MODIFICATION NO. 5. PROJECT NO. (If applicable)

1. CONTRACT ID CODE

AMENDMENT OF SOLICITATION/MODIFICATION OF CONTRACT

10/21/2022

CHECK ONE A. THIS CHANGE ORDER IS ISSUED PURSUANT TO: (Specify authority) THE CHANGES SET FORTH IN ITEM 14 ARE MADE IN THE CONTRACT

B. THE ABOVE NUMBERED CONTRACT/ORDER IS MODIFIED TO REFLECT THE ADMINISTRATIVE CHANGES (such as changes in paying office, C. THIS SUPPLEMENTAL AGREEMENT IS ENTERED INTO PURSUANT TO AUTHORITY OF:

D. OTHER (Specify type of modification and authority) appropriation data, etc.) SET FORTH IN ITEM 14, PURSUANT TO THE AUTHORITY OF FAR 43.103(b).

E. IMPORTANT: Contractor is not is required to sign this document and return __________________ copies to the issuing office.

ORDER NO. IN ITEM 10A.

14. DESCRIPTION OF AMENDMENT/MODIFICATION (Organized by UCF section headings, including solicitation/contract subject matter where feasible.)

This amendment is hereby to:

A. Post the Questions & Answers

*** Please read full solicitation notice and all attachments prior to submitting your quote.***

Pacific Walrus Genetic Analysis Testing Services for the U.S. Fish & Wildlife Service

(USFWS) Marine Mammal Office, AK

Contracting POC; Joni Dutcher, joni_dutcher@fws.gov

Continued ...

16A. NAME AND TITLE OF CONTRACTING OFFICER (Type or print)15A. NAME AND TITLE OF SIGNER (Type or print)

15C. DATE SIGNED 16B. UNITED STATES OF AMERICA 15B. CONTRACTOR/OFFEROR 16C. DATE SIGNED

(Signature of person authorized to sign) (Signature of Contracting Officer)

Joni Dutcher

STANDARD FORM 30 (REV. 11/2016)

Prescribed by GSA FAR (48 CFR) 53.243

Previous edition unusable

Except as provided herein, all terms and conditions of the document referenced in Item 9 A or 10A, as heretofore changed, remains unchanged and in full force and effect .

ITEM NO. SUPPLIES/SERVICES QUANTITY UNIT UNIT PRICE AMOUNT

NAME OF OFFEROR OR CONTRACTOR

2 3

CONTINUATION SHEET

REFERENCE NO. OF DOCUMENT BEING CONTINUED PAGE OF

(A) (B) (C) (D) (E) (F)

140F0S23Q0001/0003

Tech POC: Irina Trukhanova, irina_trukhanova@fws.gov (206) 307-7313

Alt POC: Stephanie Burgess, stephanie_burgess@fws.gov (757) 572-2949

Note: Submit all questions in writing to Joni

Dutcher at joni_dutcher@fws.gov

Note: In the event of uncontrollable occurrences

(i.e. pandemic, natural disaster, defense attack against the U.S.A, federal furlough, etc.)

affecting the completion of this contract, the contractor shall only bill for services and deliverables received up to the date and time of the occurrence. Any remaining funds will be de-obligated from the contract without further claim or compensation, including Fixed Price contracts.

Period of Performance: 12/12/2022 to 12/11/2027

NSN 7540-01-152-8067 OPTIONAL FORM 336 (4-86)

Sponsored by GSA

FAR (48 CFR) 53.110

Solicitation 140F0S23Q0001- Amendment 0003 Pacific Walrus Genetic Analysis Testing

Questions & Answer

1) Is USFWS going to provide the Dart panel to the tender's winner?

The tender’s winner will be provided with the pre-developed SNP loci, age and sex panels.

2) Is it possible to get a preview/template of the panel, or alternatively know what file format they're using?

The SNP information will include the ~ 65bp allele sequence and SNP position/nucleotide for each SNP locus.

3) Is the 3000 SNPs panel in development, developed or to be developed by the tenderer?

The 3000 SNP panel has been developed using DArTseq platform and will be shared with the tender’s winner. If the tender’s winner is unable for any reason to use a pre-developed marker, they will have to develop a new SNP panel with the same number of informative loci to be used in the sample processing.

4) Are samples going to be delivered as data and/or biological material? In the latter case, how is nucleic acid extraction going to be performed?

The samples are going to be delivered as biological material (skin) plated/stored according to the vendor’s technical requirements. The vendor is expected to perform DNA extraction from the provided samples using any available extraction methods allowing to obtain high purity DNA suitable for further genotyping.

5) Is the tenderer supposed to use only Dart technology or can it use its own resources, pipelines and technologies alternatively or in association with Dart (as long as it doesn't change it)?

The tender’s winner can use DaRT or any other technology allowing for the delivery of required genotyping products with negligible error rate and within identified timeframe.

6) Are Dart's data raw or already interpreted? In case the SNPs are going to be shared?

The pre-developed SNP panel will be shared with the tender’s winner.

7) Are the pipelines the tenderer is asked to develop (mt-DNA, age, sex, close-kin panels) going to be a parallel, replace or work with Dart panels?

The tender’s winner is expected to use the pre-developed SNP loci, age and sex panels to process the samples. The mtDNA marker will have to be developed from scratch. However, if the tender’s winner is unable for any reason to use any of the pre-developed markers, they will have to develop new marker(s) to be used in the sample processing.

8) When referring to plates in phase 3 and 4, does USFWS mean one sample per plate or one sample per well?

“Plate” refers to a standard PCR plate with 96 wells (94 samples plus 2 negative controls per plate).