B02-Requirements_TripleQuadMS-REV_A.pdf

SOLICITATION 1333ND19QNB640500

STATEMENT OF REQUIREMENTS (Rev A)

Triple Quad Mass Spectrometry Instrument with an Ultra Pressure Liquid Chromatograph

I. BACKGROUND INFORMATION

The National Institute of Standards and Technology’s (NIST); Materials Measurement Laboratory’s (MML), Chemical Sciences Division serves as the Nation's reference laboratory for chemical measurements and standards to enhance U.S. industry's productivity and competitiveness, assure equity in trade, and provide quality assurance for chemical measurements used for assessing and improving public health, safety, and the environment. Current research focus areas include the development of measurements and standards for exposure science reference materials, clinical and wildlife hormone measurement, development of chemistry measurements for plastic marine debris, and environmental per- and poly-fluorinated alkyl substances (PFAS) measurement and method development.

The Chemical Sciences Division is working to meet the growing need for the detection of Per- and Polyfluoroalkyl Substances compounds at the parts per trillion level. PFAS are unique chemicals whose physicochemical properties make them important for use in a variety of industrial and consumer products including carpets, cookware, food packaging, fire suppressants, and others. Human exposure to PFAS residues, particularly in water, has been implicated in the incidence of cancer, obesity, endocrine system disruption, and other adverse health effects.

Two specific PFAS compounds which need to be evaluated are perfluorooctanoic acid (PFOA) and perfluorooctane sulfonate (PFOS). NIST is working Department of Defense and the State of New York to establish standards for Ground and Potable Water.

II. SCOPE OF WORK

NIST requires a triple quad mass spectrometer (MS/MS) with an ultra pressure liquid chromatograph used for low end exposure science, hormone measurement, plastic marine debris, proteins, per and poly-fluorinated alkyl substances, and persistent organic contaminants. The instrument shall be able to reproducibly generate high resolution and high-quality MS and MS/MS with fragmentation with multiple reaction monitoring (MRM) in order to accurately identify unknown analytes in complex matrices. This instrument shall also give reproducible quantitative results compared to other MS/MS instruments.

The required equipment is specifically designed for high resolution mass spectrometric measurements. The required equipment consists of benchtop instruments with integrated instrument control and data analysis software. The inclusion of an Ultra High-Pressure Liquid Chromatograph (UHPLC) is required and the mass spectrometer instrument and software shall be capable of utilizing, with full software seamless integration and automation.

III. SPECIFICATIONS

The Contractor shall provide the following AB Sciex branded equipment, listed accessories, and software or its equivalent for complete instrument system control and data analysis capabilities meeting the salient characteristics as defined below:

Part Number Equipment

a. 4411247 SCIEX TRIPLE QUAD 5500 SYSTEM

b. 1023559 AGILENT1200 INSTANT PILOT

c. 5056621 AGILENT1260 INFINITY II BINARY PUMP

d. 5056622 INTERNAL SOLVENT SELECTION VALVE G1381B

e. 5046867 TOOL KIT FOR HPLC SYSTEM

f. 5039532 1260 INFINITY MULTISAMPLER 600 BAR 100uL SINGLE NE

g. 5068154 UPGRADE INFINITYLAB SAMPLE THERMOSTAT

h. 5039525 G7116B 1290 INFINITY II MULTICOLUMN THERMOSTAT WIT

i. 5056631 VALVE DRIVE G1353D

j. 5039528 2POS/6PORT VALVE HEAD RATED TO 1200 BAR FOR USE IN

k. 5032309 BENCH PEAK MS TABLE 1B

l. 5059443 Null Modem Cable - 15 Feet - DB9 Female to DB9 Fem

m. 4376878 FG, SYNERGI 4U FUSION RP80A 50X2

n. 4376887 FG, TRIAZINE STANDARD MIXTURE

o. 4406127 PPGs Chemical Standards Kit

Instrument Description Specification

a. Total concentration of 4ng/L for 5 compounds of PFOA/PFOS in water >50

b. High Resolution Triple Quad Triple Quadrupole mass spec

c. Mass Spectrometer (Capable of qualitative and quantitative analysis)

d. Mass range 5 – 1250 Da

e. Scan Speed up to 12,000 Da/s

f. Dynamic range (orders) 5

g. Sensitivity (signal to noise) Pos: 1 pg Reserpine on column S/N > 90,000:1

h. Sensitivity (signal to noise) Neg: 1 pg chloramphenicol on column, S/N > 90,000:1.

i. Mass Stability (Da) 0.1 over 24 hours

j. MRM Dwell Time (ms) 1 ms

k. # MRMs per method 4000 MRMs in sMRM mode 1250 in non-scheduled

l. Polarity switching 50 msec between positive and negative scans (with same mass resolution and mass accuracy performance as above)

m. Scan functions Full MS Full scan MS and selected ions for both Q1 and Q3, Product Ion Scan, Precursor Ion Scan, Neutral Loss or Gain Scan, MRM and sMRM,

n. Calibration Range (ng/L) for PFOA/PFOS in water

1-200

o. Linear Correlation for PFOA/PFOS in water > 0.999

p. S/N of 1ng/L of PFOA/PFOS in water >50

q. Accuracy of 1ng/L of PFOA/PFOS in water >95%

Concentration of 4ng/L for 5 compounds PFOA/PFOS.

Sums of five (5) compounds of PFOA/PFOS <= 4ng/L in drinking water

Software compatibility

The instrument software shall be compatible with Windows 7 or 10 operating systems. The instrument software shall be able to control and seamlessly integrate all of the MS and LC functionality with a single software program/package to ensure efficient workflow and transition between systems.

System Enhancements

The Contractor shall notify COR and TPOC in writing of all software upgrades for the purposes of instrument optimization not later than 60 days after an upgrade is released. The Contractor, the COR, and the TPOC will coordinate and decide if updates shall be completed by the Contractor or NIST technical personnel.

If Contractor installation is necessary to ensure proper performance of an enhancement, prior to completion of the installation, the Contractor shall test the equipment, review the equipment’s performance, and take whatever steps are necessary to ensure the equipment is functioning in accordance with the manufacturers’ specifications.

IV. DELIVERY

The Contractor shall deliver the instrument within 60 days from contract award. The Contractor shall install the instrument and demonstrate performance that meets all specifications for acceptance testing within 30 days after delivery. The Contractor shall provide training within 30 days after delivery.

The Contractor shall schedule and provide one (1) basic user training session for up to six (6) users at NIST. The training shall provide a basic demonstration of all equipment functions, maintenance, data administration, data acquisition, and troubleshooting. The training may be completed at NIST immediately after installation/set-up and on-site measurements demonstrating that no damage or misalignment issues arose during transportation and installation (as described below) but shall be completed no later than 30 days after delivery.

Deliverables Quantity Due Date Analytical instrumentation One (1) 60 days after award of this requirement.

Basic operations and maintenance training for the analytical instrument

One (1) 30 days after successful installation of this requirement

V. INSTALLATION

The Contractor shall deliver all line items to NIST, 331 Fort Johnson Road, Hollings Marine Laboratory, Charleston SC, 29412. Delivery of the instrument shall be 60 days from date of award. Normal duty hours are Monday through Friday, 8:30 a.m. to 5:00 p.m. with the exception of Federal holidays. Installation shall be completed at time of delivery. The contractor shall schedule and provide installation/set-up, instrument is to be unboxed, installed in laboratory, all instrument gases hooked up and plugged into electrical outlets. The contractor shall demonstrate all performance characteristics required for acceptance. The government shall provide the PFOA and PFOS samples, and the contractor shall run the analyses.

VI. TRAINING

The contractor shall schedule and provide one (1) basic user training session for up to six (6) users at NIST. Training should be for a minimum of 2 days for familiarization of instrument, software and running of samples at NIST. The training shall also provide a basic demonstration of all equipment functions, maintenance, data administration, data acquisition, and troubleshooting. The training may be completed at NIST, 331 Fort Johnson Road, Hollings Marine Laboratory, Charleston SC, 29412 immediately after installation/set-up and on-site measurements demonstrating that no damage or misalignment issues arose during transportation and installation (as described below), but shall be completed no later than 30 days after delivery.

VII. TESTING AND ACCEPTANCE

Prior to final acceptance of the instrument and final payment by NIST, all of the following shall be completed successfully:

a. A fully functional instrument, including all components, spares, accessories, consumables, and related hardware shall be delivered, installed, and tested in accordance with Section III Specifications;

b. The Government shall supply the PFOA and PFOS samples to be analyzed during acceptance testing. The Contractor shall run the analyses demonstrating performance.

c. All minimum specifications shall be met and successfully demonstrated per Section III Specifications;

d. All training shall be completed.

VIII. WARRANTY

The Contractor shall provide, at a minimum of one-year warranty for the system’s non-consumable parts from the date of final acceptance by the Government. The warranty shall cover at a minimum the following:

a. The complete instrument and associated components, systems, and software;

b. Onsite trouble shooting/technical support

c. All defects in materials and workmanship;

d. Parts, labor, and travel;

e. Software and firmware updates;

f. Remote support as needed to expedite a solution in the event NIST is unable to resolve a problem with performance;

g. Unlimited telephone and e-mail support for questions regarding operations during regular business hours.

The telephone support shall include response to questions about operating practices, application use, and service issues that can be diagnosed and resolved over the telephone;

h. At a minimum of two (2) preventive maintenance visits.

i. And at a minimum an additional two (2) day on-site response visits.

IX. PERIOD OF PERFORMANCE

The period of performance is 90 days from date of award.