SOW_High_Resolution_MS_-_Final.pdf

STATEMENT OF WORK

TITLE: Acquisition of High Resolution Mass Spectrometry Instrument

LAB REQUESTING SERVICE: Materials Measurement Laboratory, Materials Measurement Science Division

I. BACKGROUND INFORMATION

The National Institute of Standards and Technology’s Chemical Sciences Division serves as the Nation's reference laboratory for chemical measurements and standards to enhance U.S. industry's productivity and competitiveness, assure equity in trade, and provide quality assurance for chemical measurements used for assessing and improving public health, safety, and the environment. The Chemical Sciences Division is responsible for development and characterization of Standard Reference Materials (SRMs) to enhance chemical measurement comparability within the U.S. and to provide a basis for measurement comparability with other nations. SRMs are provided to support clinical, food, environmental, fuel, metal alloy, cement, and advanced materials analyses. Research activities are geared toward improvement of measurement capabilities to address U.S. industry and government agencies' requirements for new measurement services and reference materials. Current research focus areas include the development of measurements and standards for determination of protein-based health markers, chemical characterization of complex matrices (e.g. lipidomics and metabolomics), and for environmental contaminant analysis.

The Chemical Services Division maintains core expertise in:

• Analytical mass spectrometry,

• Analytical separation science,

• Atomic and x-ray fluorescence spectrometry,

• Gas metrology,

• Nuclear analytical methods,

• Nuclear magnetic resonance spectrometry, and

• Classical and electroanalytical chemistry.

Because of NIST’s role as the National Metrology Institute (NMI) in the U.S., the Division’s measurement capabilities must be benchmarked against the capabilities of NMIs worldwide as required by a Mutual Recognition Arrangement (MRA) established in 1999. The MRA provides a framework for obtaining reliable quantitative information on the comparability of measurement services provided by all NMIs, thereby providing governments and other parties with a technical foundation for wider agreements related to international trade, commerce, and regulatory affairs.

The Division actively participates in the International Committee of Weights and Measures (CIPM) Consultative Committee for Amount of Substance – Metrology in Chemistry (CCQM) comparisons, which address chemical measurement-related issues important for international trade, environmental, health, and safety-related decision making.

II. SCOPE OF WORK

NIST intends to acquire a high resolution mass spectrometer used for the metabolite and lipid profiling, identification of proteins by intact mass analysis, peptide mass fingerprinting, identification and structural elucidation of posttranslational modification (e.g. phosphorylation and glycosylation), and the quantification of peptides, proteins, lipids, and metabolites. The instrument must be able to reproducibly generate high resolution and high-quality MS and MS/MS in a variety of fragmentation modes in order to accurately identify unknown analytes in complex matrices.

This instrument must also give reproducible quantitative results compared to other MS/MS instruments.

The required equipment is specifically designed for high resolution mass spectrometric measurements. The required equipment consists of benchtop instruments with integrated instrument control and data analysis software. The inclusion of an HPLC or UPLC is not required, however the mass spectrometer instrument and software must be capable of utilizing, with full software seamless integration and automation, existing LC equipment specifically a Thermo Vanquish UPLC and a Dionex Ultimate 3000 UPLC.

III. SPECIFICATIONS

This is a brand name or equal requirement. Brand name or equal description is intended to be descriptive, but not restrictive and is to indicate the quality and characteristic of products that will be considered satisfactory to meet the agency’s requirement.

The Contractor shall provide the following Thermo Electron branded equipment or equivalent, and software for complete instrument system control and data analysis capabilities meeting the salient characteristics as defined below:

A. One (1) Thermo Electron Q Exactive High Resolution Mass Spectrometer (MS) with Ion Max Source and Heated Electro Spray Ionization (HESI-II) Probe (Mfr. Part Number 0726020) and the Exactive Series Pre-Installation Kit (Mfr. Part Number 1328020) or a brand with the same salient physical, functional and performance characteristics set forth below. The system shall meet or exceed the technical specifications identified below and shall be brand new (prototypes, demonstration models, used or remanufactured equipment will not be considered for award):

a. High resolution orbitrap mass spectrometer with quadrupole mass filter (capable of parallel filling to increase instrument duty cycle)

b. The footprint must not exceed the dimensions of 40 in x 35 in x 36 in (height x depth x width).

c. Resolution - 130,000 (FWHM) @ 200 m/z 40,000 (FWHM) @ 1000 m/z

d. Mass range - 50 – 4000 Da

e. Dynamic range (full MS scan) – 5,000

f. Sensitivity (signal to noise) - 100:1 of 500 fg reserpine (Full MS)

g. Mass accuracy - 5 ppm (external) 1 ppm (internal calibration)

h. Polarity switching - 1 sec between positive and negative scans (with same mass resolution and mass accuracy performance as above)

i. MS/MS Fragmentation - higher energy collisional dissociation (HCD)

j. Scan functions must include:

Full MS Selected ion monitoring (SIM)

Selected reaction monitoring (SRM) Multiplexed SRM Multiplexed SIM Data dependent analysis (DDA) Data Independent Analysis (DIA) Neutron Loss Triggered DDA Timed SIM Timed SRM Dynamic exclusion

k. HESI/ESI Source operating voltage - up to ± 6000 V (100 µamps)

l. HESI/ESI Liquid flow rate operation range - 1 µL/min – 1000 µL/min

m. HESI/ESI Temperature up to 400 ºC

The instrument software must be compatible with Windows 7 operating systems. The instrument software must be able to control and seamlessly integrate all of the MS and LC functionality (i.e. existing Thermo Vanquish UPLC and a Dionex Ultimate 3000 UPLC) with a single software program/package to ensure efficient workflow and transition between systems.

Warranty:

The Contractor shall provide, at a minimum, a three-year warranty for the system’s non-consumable parts. The warranty shall cover the following:

- Parts, labor, and travel;

- Software and firmware updates;

- Unlimited, free email and phone support;

- In the event that NIST is unable to resolve a problem, the Contractor shall provide remote support as needed to expedite a solution for NIST;

- Telephone support for the covered equipment during regular business hours; Telephone support must include response to questions about operating practices, application use, and service issues that can be diagnosed and resolved over the telephone.

The warranty shall commence upon successful delivery, training and demonstration of all salient characteristics.

Trade-in:

NIST is offering its existing Thermo Velos Pro mass spectrometer as a trade-in. The existing Thermo Velos Pro system was purchased from Thermo Scientific and is approximately 5 years old. This system, serial # LTQ40417 is fully functioning and working order. Offerors interested in offering a discount for the trade-in of this existing system should note the trade-in discount as a line item on their quotation.

Specific Tasks

The Contractor shall provide all support for equipment, administration, and technical execution of this contract. The Contractor is responsible for maintaining accurate records of maintenance and repair activities. The Contractor shall perform the following tasks in support of the scope of work listed in Section II of this Statement of Work (SOW)

Installation/Training/Warranty

• The contractor shall schedule and facilitate installation/set-up and one (1) basic user training session for up to three (3) users at NIST. The training shall provide a basic demonstration of all equipment functions, maintenance, data administration, data acquisition, and troubleshooting. The training may be completed at NIST immediately after installation/set-up and on-site measurements demonstrating that no damage or misalignment issues arose during transportation and installation (as described below), but shall be completed no later than 30 days after delivery.

• The Contractor shall provide, at a minimum, a three (3) year warranty for the system. The warranty must cover the complete instrument and associated components, systems, and software.

The warranty must cover all defects in materials and workmanship for a period of one year from the date of final acceptance by the Government.

• Quotation must include unlimited telephone/e-mail support, during the warranty period, for questions regarding operation, and a three-year warranty. This warranty must cover non-consumable parts and travel/labor on-site should this become necessary within the warranty period.

Quotation must include the number and the home station of the service engineers who are responsible for the area that covers Charleston, SC Preventive Maintenance

System Enhancements

Install, or if NIST technical personnel can install the upgrades without compromising system performance make available for installation, all software upgrades for the purposes of instrument optimization not later than 60 days after any such upgrade is released.

If contractor installation is necessary to ensure proper performance, prior to completion of the installation, the Contractor shall test the equipment, review the equipment’s performance, and take whatever steps are necessary to ensure the equipment is functioning in accordance with the manufacturers’ specifications.

PERIOD OF PERFORMANCE

Delivery for the equipment shall be 30 days after award and installation and training shall be 30 days after delivery.

Warranty for the equipment shall be three (3) years after acceptance of the equipment.

IV. PLACE OF PERFORMANCE

All work shall be completed at the Contractor’s facility. Delivery of the instrument shall be at NIST, Charleston, SC. Normal duty hours are Monday through Friday, 8:30 a.m. to 5:00 p.m.

with the exception of Federal holidays. Installation and training are required with this instrumentation.

V. DELIVERABLES

Description Quantity Due Date

Analytical instrument One (1) 30 days after award of this requirement.

Installation of the analytical instrument Once 30 days after delivery Operations and maintenance manual for the analytical instrument

One (1) 30 days after award of this requirement

Basic operations and maintenance training for the analytical instrument

Once 30 days after delivery

Warranty Three (3) years

Commencing after final acceptance of fully functioning analytical instrument

VI. PERFORMANCE REQUIREMENT SUMMARY

Prior to final acceptance of the instrument and final payment by NIST, all of the following shall be completed successfully:

• A fully functional instrument, including all components, spares, accessories, consumables, and related hardware shall be delivered, installed, and tested in accordance with the minimum specifications described herein;

• All minimum specifications must be met and all performance metrics must be successfully demonstrated on the delivered instrument;

• All documentation shall be delivered in both electronic and hardcopy formats;

• All training shall be completed.

• The contractor shall pack and mark the shipment in conformance with carrier requirements, deliver the shipment in good order and condition to the point of delivery specified in the purchase order, be responsible for any loss of and/or damage to the goods occurring before receipt and acceptance of the shipment by the consignee at the delivery point specified in the purchase order; and pay all charges to the specified point of delivery. The contractor shall deliver all line items to NIST, 331 Fort Johnson Road, Hollings Marine Laboratory, Charleston SC, 29412.