Sol_1333MF24Q0094_Amd_0002.pdf

COMBINED SYNOPSIS/SOLICITATION

Description: Amendment—Revised Technical Specifications and Delivery Date. Purchase of an Applied Biosystems QuantStudio 3 Real-Time PCR System

(i) This is a combined synopsis/solicitation for commercial items prepared in accordance with the format in FAR Subpart 12.6, as supplemented with additional information included in this notice and in accordance with the simplified acquisition procedures authorized in FAR Part 13. This announcement constitutes the only solicitation; quotes are being requested and a written solicitation will not be issued.

(ii) This solicitation is issued as a request for quotation (RFQ). Submit written quotes on RFQ Number: 143071-24-0072.

(iii) The solicitation document and incorporated provisions and clauses are those in effect through Federal Acquisition Circular 2024-05 (May 2024).

(iv) This solicitation is being issued as Unrestricted and open to all sources. The associated NAICS code is 334516. The small business size standard is 1000 employees.

(v) This combined solicitation/synopsis is for purchase of the following commercial supply:

CLIN 0001:

Purchase of Applied Biosystems QuantStudio 3 Real-Time PCR System in accordance with the Statement of Need. Deliver by November 30, 2024.

(vi) Description of requirements is as follows: See attached Statement of Need which applies to this contract.

(vii) Date(s) and place(s) of delivery and acceptance:

Deliver by November 30, 2024.

Delivery shall be FOB Destination to:

NOAA Pacific Islands Fisheries Science Center 1845 Wasp Blvd., Bldg. 176 Honolulu, Hawaii 96818

Place of Performance is: Contractor’s Facility.

(viii) FAR provision 52.212-1, Instructions to Offerors-Commercial Products and Commercial Services (Sept 2023), applies to this acquisition and can be found on the Request for Quote form SF18 attachment.

(ix) FAR provision 52.212-2, Evaluation-Commercial Products and Commercial Services (Nov 2021), applies to this acquisition and can be found on the Request for Quote form SF18 attachment.

(x) FAR provision 52.212-3, Offeror Representations and Certifications-Commercial Products and Commercial Services (May 2024), applies to this acquisition and can be found on the Request for Quote form SF18 attachment. Offerors are advised to include a completed copy of the provision with its offer.

(xi) FAR clause at 52.212-4, Contract Terms and Conditions-Commercial Products and Commercial Services (Nov 2023), applies to this acquisition and can be found on the Request for Quote form SF18 attachment.

https://www.acquisition.gov/far/part-52#FAR_52_212_1 https://www.acquisition.gov/far/part-52#FAR_52_212_2 https://www.acquisition.gov/far/part-52#FAR_52_212_3 https://www.acquisition.gov/far/part-52#FAR_52_212_4

(xii) FAR clause at 52.212-5, Contract Terms and Conditions Required To Implement Statutes or Executive Orders-Commercial Products and Commercial Services (May 2024), applies to this acquisition apply can be found in full text on the Request for Quote form SF18 attachment.

(xiii) Any additional contract requirement(s) or terms and conditions that apply can be found on the Request for Quote form SF18 attachment.

(xiv) Defense Priorities and Allocations System (DPAS) and assigned rating does not apply.

(xv) Quotes are required to be received in the contracting office no later than 4:30PM HST on September 13, 2024. All quotes must be submitted electronically via email to Andrew.choy@noaa.gov.

(xvi) Any questions regarding this solicitation should be submitted electronically via email to:

Andrew.choy@noaa.gov.

(End)

1352.215-72 Inquiries (Apr 2010)

OFFERORS MUST SUBMIT ALL QUESTIONS CONCERNING THIS SOLICITATION IN WRITING

VIA EMAIL TO: ANDREW.CHOY@NOAA.GOV. QUESTIONS SHOULD BE RECEIVED NO

LATER THAN SEPTEMBER 11, 2024. Any responses to questions will be made in writing, without identification of the questioner, and will be included in an amendment to the solicitation. Even if provided in other form, only the question responses included in the amendment to the solicitation will govern performance of the contract.

(End of clause)

52.237-1 Site Visit (Apr 1984) Offerors or quoters are urged and expected to inspect the site where services are to be performed and to satisfy themselves regarding all general and local conditions that may affect the cost of contract performance, to the extent that the information is reasonably obtainable. In no event shall failure to inspect the site constitute grounds for a claim after contract award.

(End of provision)

A site visit is highly recommended but not required. Offerors are urged and expected to inspect the site where services are to be performed and to satisfy themselves regarding all general and local conditions that may affect the cost of contract performance, to the extent that the information is reasonably obtainable. In no event shall failure to inspect the site constitute grounds for a claim after contract award.

Please contact Hope Ronco (Hope.Ronco@noaa.gov) to set up a time to visit the site. All questions during the site visit should be submitted electronically to: Andrew.choy@noaa.gov.

FAR 52.212-1 Instructions to Offerors-Commercial Products and Commercial Services (Sep 2023) NOTICE TO OFFERORS: Instructions for submitting quotations under this request for quote must be followed. Failure to provide all information to aid in the evaluation may be considered non-responsive. Offers that are non-responsive may be excluded from further evaluation and rejected without further notification to the offeror.

1. Submit PDF or Microsoft Word format quotations to the office specified in this solicitation at or before the time specified in the solicitation. Email quotes are required and can be sent to:

ANDREW.CHOY@NOAA.GOV.

https://www.acquisition.gov/far/part-52#FAR_52_212_5 mailto:Andrew.choy@noaa.gov mailto:ANDREW.CHOY@NOAA.GOV mailto:Andrew.choy@noaa.gov

2. Offeror shall have an active registration in the System for Award Management (SAM found at https://sam.gov/content/home) in order to provide a quote and be eligible for award. Must provide their UEID/CAGE CODE with their quote.

3. Offerors shall assume that the Government has no prior knowledge of them or their capability.

4. Offerors must provide all evaluation criteria in accordance with FAR 52.212-2. Offerors who do not provide all evaluation criteria will not be accepted.

THE GOVERNMENT DOES NOT ACCEPT RESPONSIBILITY FOR NON-RECEIPT

OF QUOTES. IT IS THE CONTRACTOR’S RESPONSIBILITY TO REQUEST AND

RECEIVE A CONFIRMATION OF THE QUOTE RECEIPT.

FAR 52.212-2 Evaluation- Commercial Products and Commercial Services (Nov 2021) Offers will be evaluated based on price and the factors set forth in paragraph (a).

(a) The Government will award a contract resulting from this solicitation to the responsible offeror whose offer conforming to the solicitation will be most advantageous to the Government, price and other factors considered. Paragraph (a) is hereby completed as follows: Evaluation will be based on the following;

1. Specification Sheet

2. Price.

The Government intends to award a low priced, technically acceptable, single firm fixed-price purchase order on an all or none basis with payment terms of Net 30.

(b) Options. The Government will evaluate offers for award purposes by adding the total price for all options to the total price for the basic requirement. The Government may determine that an offer is unacceptable if the option prices are significantly unbalanced. Evaluation of options shall not obligate the Government to exercise the option(s).

(c) A written notice of award or acceptance of an offer, mailed or otherwise furnished to the successful offeror within the time for acceptance specified in the offer, shall result in a binding contract without further action by either party. Before the offer’s specified expiration time, the Government may accept an offer (or part of an offer), whether or not there are negotiations after its receipt, unless a written notice of withdrawal is received before award.

(End of provision) https://sam.gov/content/home

AMENDED STATEMENT OF NEED

143071-24-0072

Applied Biosystems QuantStudio 3 Real-Time PCR System

PURPOSE:

The National Oceanic and Atmospheric Administration (NOAA) Pacific Islands Fisheries Science Center (PIFSC) Ecosystem Sciences Division (ESD) seeks to purchase one a quantitative real-time PCR (qPCR) thermalcycler to support research identifying genetic material in tissues and environmental samples.

We require the purchase of the Applied Biosystems QuantStudio 3 Real-Time PCR System manufactured by Life Technologies Corporation to maintain consistency of existing qPCR projects that utilize the QuantStudio platform and reagents. The QuantStudio 3 qPCR system is a high-performance real-time quantitative PCR system with 96-well sample throughput.

BACKGROUND:

Environmental DNA (eDNA) is a powerful and emerging tool that can complement existing surveys to expand and refine our understanding of fish populations and habitat use in marine ecosystems. Currently, eDNA techniques are in the process of becoming a part of the standard fishery and wildlife management toolkit for population detection, assessment, and monitoring.

Large-scale eDNA surveys can be cost-effective ways for assessing occupancy and relative abundance of target fisheries and protected species in habitats and locations not currently surveyed by other techniques. eDNA studies commonly use quantitative polymerase chain reaction (qPCR) for the detection of low levels of target species' eDNA found in complex environmental samples such as a water sample (e.g., Shelton et al. 2022). PIFSC does not currently have access to a qPCR thermalcylcer, and thus would like to procure one to permit these types of surveys.

qPCR is one of the most common techniques for quantification of nucleic acid molecules in biological and environmental samples. In real-time PCR, the accumulation of amplification product is measured as the reaction progresses, in real time, with product quantification after each cycle. First, amplification reactions are set up with PCR reagents and unique custom-designed primers. Reactions are then run in real-time PCR instruments. Detection of PCR products is enabled by the inclusion of a fluorescent reporter molecule in each reaction that yields increased fluorescence with an increasing amount of product DNA. Fluorescently-labeled sequence-specific probes are designed to particular gene regions that selectively amplify target species. When primers and probes are designed to be species-specific, fluorescence occurs only when the forward primer, reverse primer and probe hybridize to the target species DNA.

Specialized thermal cyclers equipped with fluorescence detection modules are used to monitor the fluorescence signal as amplification occurs. The measured fluorescence is proportional to the total amount of amplicon; the change in fluorescence over time is used to calculate the amount of amplicon produced in each cycle. The main advantage of real-time PCR over PCR is that real-time PCR allows you to determine the initial number of copies of template DNA (the amplification target sequence) with accuracy and high sensitivity. Real-time PCR results can either be qualitative (the presence or absence of a sequence) or quantitative (copy number).

Quantitative real-time PCR is thus also known as qPCR analysis. Probe-based qPCR provides the opportunity to process multiple samples in a single reaction (multiplex).

To conduct large-scale eDNA surveys and have the ability to develop species-specific qPCR assays in house, PIFSC requires the procurement of a qPCR thermalcycler. We require the purchase of the Applied Biosystems QuantStudio 3 Real-Time PCR System manufactured by Life Technologies Corporation to maintain consistency of existing qPCR projects that utilize the QuantStudio platform and reagents. For example, we have developed species-specific qPCR primer-probe assays to identify Hawaiian Bottomfish from environmental samples using the Applied Biosystems QuantStudio Real-Time PCR system using TaqMan Environmental Master Mix and TaqMan multiple kits.

REVISED TECHNICAL SPECIFICATIONS:

Quantity Unit Catalog Number Description

1 EA A28566 AB QS 3 RTPCR 96WELL0.1 LAP

1 EA 4351979 FAST 96 WELL RNASE P VERIF

1 EA 4483485 FAST 96W HARDSHELL PLATE CLEAR

1 EA 4360954 OPTICAL ADHESIVE COVERS

1 EA A26774 KIT 802.11B/G/N WIFI DONGLE EA

1 EA A46012 POWERTRACK SYBR MM 1ML

1 EA 4444556 TAQMAN FAST ADVANCED MMIX

1 EA A28806 SMARTSTART QSTUDIO3 EACH

1 EA Freight FOB Destination to: 1854 Wasp Blvd. Bldg 176, Honolulu, HI 96818 1 EA N/A AB Assurance Plan

DELIVER BY: November 30, 2024

DELIVER FOB DESTINATION TO:

NOAA Pacific Islands Fisheries Science Center 1845 Wasp Blvd., Bldg. 176 Honolulu, Hawaii 96818

(x)

1333MF24Q0094

x x copies of the amendment; (b) By acknowledging receipt of this amendment on each copy of the offer submitted ; or (c) By separate letter or electronic communication which includes a reference to the solicitation and amendment numbers. FAILURE OF YOUR ACKNOWLEDGEMENT TO BE

RECEIVED AT THE PLACE DESIGNATED FOR THE RECEIPT OF OFFERS PRIOR TO THE HOUR AND DATE SPECIFIED MAY RESULT IN REJECTION OF YOUR

OFFER. If by virtue of this amendment you desire to change an offer already submitted , such change may be made by letter or electronic communication, provided each letter or electronic communication makes reference to the solicitation and this amendment, and is received prior to the opening hour and date specified.

x

NMFS-FD

KANSAS CITY MO 64106

601 EAST 12TH STREET SUITE 1746

NMFS Field Delegates

09/06/20240002

13. THIS ITEM ONLY APPLIES TO MODIFICATION OF CONTRACTS/ORDERS. IT MODIFIES THE CONTRACT/ORDER NO. AS DESCRIBED IN ITEM 14.

12. ACCOUNTING AND APPROPRIATION DATA (If required) is not extended.is extended, Items 8 and 15, and returning

Offers must acknowledge receipt of this amendment prior to the hour and date specified in the solicitation or as amended , by one of the following methods: (a) By completing

The above numbered solicitation is amended as set forth in Item 14. The hour and date specified for receipt of Offers

11. THIS ITEM ONLY APPLIES TO AMENDMENTS OF SOLICITATIONS

FACILITY CODE CODE

10B. DATED (SEE ITEM 13)

10A. MODIFICATION OF CONTRACT/ORDER NO.

9B. DATED (SEE ITEM 11)

9A. AMENDMENT OF SOLICITATION NO.

CODE

8. NAME AND ADDRESS OF CONTRACTOR (No., street, county, State and ZIP Code)

7. ADMINISTERED BY (If other than Item 6)CODE 6. ISSUED BY

PAGE OF PAGES

4. REQUISITION/PURCHASE REQ. NO.3. EFFECTIVE DATE2. AMENDMENT/MODIFICATION NO. 5. PROJECT NO. (If applicable)

1. CONTRACT ID CODE

AMENDMENT OF SOLICITATION/MODIFICATION OF CONTRACT

07/12/2024

CHECK ONE A. THIS CHANGE ORDER IS ISSUED PURSUANT TO: (Specify authority) THE CHANGES SET FORTH IN ITEM 14 ARE MADE IN THE CONTRACT

B. THE ABOVE NUMBERED CONTRACT/ORDER IS MODIFIED TO REFLECT THE ADMINISTRATIVE CHANGES (such as changes in paying office, C. THIS SUPPLEMENTAL AGREEMENT IS ENTERED INTO PURSUANT TO AUTHORITY OF:

D. OTHER (Specify type of modification and authority) appropriation data, etc.) SET FORTH IN ITEM 14, PURSUANT TO THE AUTHORITY OF FAR 43.103(b).

E. IMPORTANT: Contractor is not is required to sign this document and return __________________ copies to the issuing office.

ORDER NO. IN ITEM 10A.

14. DESCRIPTION OF AMENDMENT/MODIFICATION (Organized by UCF section headings, including solicitation/contract subject matter where feasible.)

Amendment—Revised Technical Specifications and Delivery Date. See Amended Statement of Need.

16A. NAME AND TITLE OF CONTRACTING OFFICER (Type or print)15A. NAME AND TITLE OF SIGNER (Type or print)

15C. DATE SIGNED 16B. UNITED STATES OF AMERICA 15B. CONTRACTOR/OFFEROR 16C. DATE SIGNED

(Signature of person authorized to sign) (Signature of Contracting Officer)

ANDREW CHOY

STANDARD FORM 30 (REV. 11/2016)

Prescribed by GSA FAR (48 CFR) 53.243

Previous edition unusable

Except as provided herein, all terms and conditions of the document referenced in Item 9 A or 10A, as heretofore changed, remains unchanged and in full force and effect .