Attachment_3_-_RFI_Tracker_-_RNA_dated_04_22_2026_0002.xlsx
XLSX spreadsheet 68 KB Posted
- Attached to
- Non-Personal Services RNA Sequencing
- Solicitation number
- 1305M326Q0132
About this file
This is an RFI (Request for Information) Tracker documenting eleven clarifying questions and responses related to NOAA's RNA Sequencing solicitation (1305M326Q0132) issued in April 2026. The solicitation requires vendors to perform RNA extraction, library preparation, and next-generation sequencing on forty purified RNA samples derived from sea scallop tissue preserved in DNA/RNA shield. All samples will be delivered in a single shipment, and vendors must complete sequencing and data delivery within sixty days of sample receipt.
The technical requirements specify Illumina RNA Library Preparation with Poly-A enrichment using a NovaSeq 6000 sequencer with paired-end 150 base pair reads (PE150) at 6 million reads per library (1GB per sample), and 3'Tag-Seq poly-A library preparation methodology. Vendors must deliver fast.gz files for all sequenced samples and provide documentation of initial quality control results, sample pass/fail status for sequencing, and turnaround time capabilities. Key evaluation criteria include published experience with the requested services, documented QC procedures, turnaround time, available sequencing platform capabilities, RNA extraction capacity from tissue samples, and library preparation expertise. The performance period spans sixty days from sample receipt, with all RFI responses provided to contractors on April 22, 2026.
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Sheet1
| Requests For Information (RFIs) | ||||||
| Project Title: RNA Sequencing | ||||||
| RFQ/RFP Number: 1305M326Q0132 | ||||||
| RFI # | Date Recv'd | RFI | Date Sent to Client | Client/SME Response | Response Date | Date sent to Ktrs |
| 1 | 04/16/26 | Could you please confirm whether the RNA samples will be delivered in multiple batches or all at once for processing? | 04/16/26 | All samples will be delivered at once. | 4/21/26 | 04/22/2026 |
| 2 | 04/16/26 | Could you advise on the required read length (in base pairs) for the single-end sequencing, as the Statement of Work specifies coverage but does not indicate read length? | 04/16/26 | Read length for tag seq is typically 150bp from the poly a tail, including adapters | 4/21/26 | 04/22/2026 |
| 3 | 04/17/26 | The Statement of Work references 3’Tag-Seq poly A library preparation with single-end reads, while the CLIN description references Illumina RNA library preparation with Poly-A enrichment and NovaSeq PE150 (6M reads/library). Could you please confirm which sequencing method and read configuration is required? | 04/17/26 | Illumina RNA Library Preparation w/Poly-A Enrichment, 1Gb Illumina NovaSeq 6000 PE 150 (6M Reads/Library: RNA extraction, 3’Tag-Seq poly A RNA library preparation, and Next Generation Sequencing of prepared libraries. Delivery of sequence data from forty (40) purified RNA samples extracted from sea scallop tissue. | 04/21/2026 | 04/22/2026 |
| 4 | 04/17/26 | Will all samples be delivered as purified RNA, or should offerors anticipate receiving tissue samples preserved in RNAlater or similar fixatives that would require RNA extraction? | 04/17/26 | all samples are tissue fixed in DNA rna shield, we expect the vendor to extract rna, prep libraries, and sequence | 04/21/2026 | 04/22/2026 |
| 5 | 04/17/26 | In addition to pricing, could you please confirm the expected level of detail for the technical narrative (e.g., page limits, specific evaluation elements, or preferred format)? | 04/17/26 | Fast.gz files for every sequenced sample that can be transferred. No page limit to technical review but must include initial QC, samples that passed and failed for sequencing. | 4/21/26 | 04/22/2026 |
| 6 | 04/17/26 | Could you clarify what specific factors NOAA will use to determine technical acceptability (e.g., prior experience, sequencing platform capabilities, QC procedures, publications, or sample handling approach)? | 04/17/26 | cited publication history using all requested services, QC procedure, turnaround time to fulfill services, sequencing platform available, can tissues to extract rna, library prep capabilities | 4/21/26 | 04/22/2026 |
| 7 | 04/17/26 | Will all 40 samples be shipped at once, or should offerors anticipate multiple shipments corresponding to the four experimental batches? | 04/17/26 | One shipment of all samples | 4/21/26 | 04/22/2026 |
| 8 | 04/17/26 | The SOW indicates delivery of results within 60 days of sample receipt. Does this timeline apply to each batch individually or to the full set of samples collectively? | 04/17/26 | Full set of samples collectively. | 04/21/26 | 04/22/2026 |
| 9 | 04/17/26 | The solicitation notes that the period of performance is TBD. Should offerors base their pricing and scheduling solely on the 60-day turnaround requirement, or will a defined performance period be established at award? | 04/17/26 | The award of performance would be 60 days after receiving the samples | 04/21/26 | 04/22/2026 |
| 10 | 04/20/26 | Will we receive 4 x 40 samples for a total of 160? Or 4 x 10 samples for a total of 40? | 04/20/26 | 40 samples total for illumina RNA library prepartion w poly-A enrichement and 80 1 GB illumina NOVseq 6000 PE 150 (6M reads/library) | 04/21/26 | 04/22/2026 |
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