RFI No. 01 QandA.pdf

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12905B23Q0087 Whole transcriptome sequencing of

USDA, ARS, FRRL

690 North 1100 East Logan, UT 834322-6300

Various

01 05/25/2023

Question 1. If our workflow is Plant total RNA sequencing with ribosomal depletion, is this acceptable?

Question 2. Have these samples been infected with any sort of pathogens (BSL-2 virus, bacteria, etc.)?

Question 3. Sample Origin?

Question 4. Species?

Question 5. Does this SOW need data analysis?

Question 6. What type of files do they need at the end? Raw mapped data like fastq? SAM? BAM? Or annotated read count matrix?

Question 7. Will the 3 library sets be sent at separate times? Or can these sets be prepared and sequenced as a single 112 sample batch?

Whole transcriptome sequencing - 12905B23Q0087

USDA/ARS/PWA–Shelley Steen shelley.steen@usda.gov

COR Comments:

Answer 1. Yes we will provide total RNA and ribosomal depletion is just fine.

Answer 2. No pathogens whatsoever. Just plant samples grown in a greenhouse.

Answer 3. Sample Origin is Logan, UT, USA

Answer 4. Species is Lolium perenne, or perennial ryegrass

Answer 5. No data analysis needed

Answer 6. fastq files are fine

Answer 7. Either way can be done. The third set of 32 samples will be ready on July 10, while the other two sets are ready immediately. I can wait and submit all in July, or piecemeal.