Sequencing SOW 2.pdf

Statement of Work

The facility will provide a range of sequencing services, including Sanger sequencing, Illumina-based metabarcoding, metagenomic and metatranscriptomic sequencing and Nanopore-based genomic sequencing, plus library preparation for Illumina and Nanopore. Additional services include quality checks for DNA and RNA to ensure successful library generation and sequencing.

Specific work to be done:

Sanger sequencing of full-length 16S, LSU and other DNA targets, minimum 1500 bp length (estimate two 96 well plates, total 192 samples) Illumina MiSeq sequencing with V3 2 x 300 bp paired end sequencing (estimate 6 sequencing runs) Illumina metagenomic library preparation with Illumina TruSeq Nano DNA kit (estimate 36 samples) Illumina metatranscriptomic library preparation with Qiagen QIAseq FastSelect rRNA depletion with TruSeqStranded Total RNA library kit (estimate 24 samples) Sequencing via Illumina NovaSeq S4 flowcell 2 x 150 bp paired end (estimate 6 sequencing runs) Nanopore library preparation via 1D ligation sequencing kit with barcoding for multiplexing of samples (estimate 3 barcode library preps, total 18 samples) Nanopore sequencing via FLO-MINI114 for sequencing on GridION or PrometION (estimate 3 sequencing runs) QC of libraries via Bioanalyzer or Tape station (estimate 10 pooled libraries)

Review of deliverables:

The POC will deliver prepared libraries or amplicons, or raw DNA or RNA for either library preparation or sequencing via the above technologies, depending upon the application. The facility will perform sample quality checks on libraries, amplicons or nucleic acids and perform sequencing as required.