127EAU26Q0080 Specifications Sheet.pdf

This is a Request for Quote (RFQ) for an automated DNA analysis and size selection pulse field electrophoresis system, issued by the USDA Agricultural Research Service. The RFQ number is 127EAU26Q0080, with the technical point of contact being Scott Geib (808-491-3861, scott.geib@usda.gov). The equipment is to be shipped to USDA-ARS, 64 Nowelo Street, Hilo, HI 96720.

The requested system must meet or exceed the following minimum specifications: automated gel-based DNA size selection for DNA and prepared sequencing libraries with the ability to recover selected DNA for downstream sequencing or analysis; pulsed-field electrophoresis or equivalent capability for separating and analyzing large DNA fragments; fragment-length analysis capability up to at least 80 kb; DNA quantification using fluorometric or equivalent methodology; ability to process sample lanes independently using different size-selection parameters or cutoffs; use of pre-prepared gel cassettes or equivalent ready-to-use consumables; ability to preserve unused lanes or sample positions for later use; compatibility with next-generation sequencing workflows; and all necessary software and hardware for system operation and results analysis. Installation, setup, calibration, and customer training must be included with the equipment. The RFQ references LightBench Discover or similar products as acceptable solutions.