SF 30_Amendment_1_12639521Q0145.pdf

AMENDMENT OF SOLICITATION/MODIFICATION OF CONTRACT

1. CONTRACT ID CODE PAGE OF PAGES

1 2

2. AMENDMENT/MODIFICATION NO.

3. EFFECTIVE DATE

05/18/2021

4. REQUISITION/PURCHASE REQ. NO. 5. PROJECT NO. (If applicable)

6. ISSUED BY CODE 7. ADMINISTERED BY (If other than Item 6) CODE

Carol Dingess Contracting Officer USDA/MRP/Acquisition and Asset Management Division Email: carol.dingess@usda.gov

Carol Dingess Contracting Officer USDA/MRP/Acquisition and Asset Management Division Email: carol.dingess@usda.gov

8. NAME AND ADDRESS OF CONTRACTOR (No., street, county, State and ZIP Code)

Contractor Name

DUNS#

POC Name

Ph#

Email

(X) 9A. AMENDMENT OF SOLICITATION

NO.

12639521Q0145

9B. DATED (SEE ITEM 11)

04/26/2021

10A. MODIFICATION OF CONTRACT/ORDER NO.

10B. DATED (SEE ITEM 13)

CODE FACILITY CODE

11. THIS ITEM ONLY APPLIES TO AMENDMENTS OF SOLICITATIONS

The above numbered solicitation is amended as set forth in Item 14. The hour and date specified for receipt of Offers is extended, is not extended.

Offers must acknowledge receipt of this amendment prior to the hour and date specified in the solicitation or as amended, by one of the following methods:

(a) By completing items 8 and 15, and returning copies of the amendment; (b) By acknowledging receipt of this amendment on each copy of the offer submitted;

or (c) By separate letter or telegram which includes a reference to the solicitation and amendment numbers. FAILURE OF YOUR ACKNOWLEDGMENT TO BE RECEIVED AT THE PLACE DESIGNATED FOR THE RECEIPT OF OFFERS PRIOR TO THE HOUR AND DATE SPECIFIED MAY RESULT IN REJECTION OF YOUR OFFER. If by virtue of this amendment your desire to change an offer already submitted, such change may be made by telegram or letter, provided each telegram or letter makes reference to the solicitation and this amendment, and is received prior to the opening hour and date specified.

12. ACCOUNTING AND APPROPRIATION DATA (If required)

13. THIS ITEM ONLY APPLIES TO MODIFICATION OF CONTRACTS/ORDERS.

IT MODIFIES THE CONTRACT/ORDER NO. AS DESCRIBED IN ITEM 14.

CHECK ONE A. THIS CHANGE ORDER IS ISSUED PURSUANT TO: (Specify authority) THE CHANGES SET FORTH IN ITEM 14 ARE MADE IN THE CONTRACT ORDER NO.

IN ITEM 10A.

B. THE ABOVE NUMBERED CONTRACT/ORDER IS MODIFIED TO REFLECT THE ADMINISTRATIVE CHANGES (such as changes in paying office, appropriation date, etc.) SET FORTH IN ITEM 14, PURSUANT TO THE AUTHORITY OF FAR 43.103(b).

C. THIS SUPPLEMENTAL AGREEMENT IS ENTERED INTO PURSUANT TO AUTHORITY OF:

D. OTHER (Specify type of modification and authority)

E. IMPORTANT: Contractor is not, is required to sign this document and return copies to the issuing office.

14. DESCRIPTION OF AMENDMENT/MODIFICATION (Organized by UCF section headings, including solicitation/contract subject matter where feasible.)

Solicitation Amendment IAW FAR 13.307 (C )(3)

Amentment_1 Q & A Posted to Beta.SAM RFQ 12639521Q0145 . Solicitation is hereby extended to 05/24/2021 closing date

Lack of submission of any or all documentation requested will result in no further consideration of quote package.

Except as provided herein, all terms and conditions of the document referenced in Item 9A or 10A, as heretofore changed, remains unchanged and in full force and effect.

15A. NAME AND TITLE OF SIGNER (Type or print) 16A. NAME AND TITLE OF CONTRACTING OFFICER (Type or print)

15B. CONTRACTOR/OFFEROR

(Signature of person authorized to sign)

15C. DATE SIGNED 16B. UNITED STATES OF AMERICA

(Signature of Contracting Officer)

16C. DATE SIGNED

NSN 7540-01-152-8070

Previous edition unusable

STANDARD FORM 30 (REV. 10-83)

Prescribed by GSA FAR (48 CFR) 53.243

X X

CAROL DINGESS

mailto:carol.dingess@usda.gov

Beta.SAM Q & A RFQ 12639521Q0145 extended to 05/24/2021

Q_1 - Is this contract only for sanger DNA sequencing, or is there also next-generation sequencing involved?

A - Just sanger sequencing for this contract.

Q_2 - As for pricing schedule, can you please clarify scope of work for fragment analysis?

A - We will be performing the PCR with tagged end labeled primer (6-Fam or Vic most common so far. Not sure if may add another dye) which are used for microsatellite analysis.

In particular, we hope to estimate fragment size based on fragment migration compared to a known ladder.

To date, all fragment analysis has been performed on an ABI 3730XL DNA Analyzer. We hope that this or equivalent is being used. We have not validated our methods using other machines.

We will dilute (usually 1:10, but this can be adjusted as needed). We would send about 20 ul of the diluted sample (unless more is requested) for analysis, primers present (we do not clean the PCR product; all analysis has been done with primers present and should not be cleaned) with expected fragment lengths between 60bp and 300bp

We will need it run with a ladder for size selection (GS500 or equivalent provided by the contractor) and resulting files (FSA file) sent to us.

After we get the results and we analyze data using PeakScanner, we may request further dilution of the sample sent (in case of signal strength is to high (sometimes occur since some samples are done in multiplex)

Samples are sent in as either plate of various specimens (48 - 94 samples with 6 multiplexed panels is our most common usage) or as singles specimens needing the 6 multiplexed panels

Q_3 Should we perform from DNA extraction to data analysis?

A - No. We will be providing the sample as needed for analysis. In case of sequencing, We will provide the DNA template with primer mixed. Unless otherwise requested by company, our standard protocol has been to send 2.5 ul purified DNA template (~20 ng/ul), 5 ul 5 umol Primer, and 7.5 ul of water. After sequencing, data is sent to us and we analyze the data ourselves. Extra DNA is sent in case a repeat is needed.