Single Nucleotide Polymorphisms Genotyping Service - Questions and Answers.pdf

Questions and Answers – Single Nucleotide Polymorphisms Genotyping Service

1. Q: Can you confirm that your USDA group will provide the contractor of the solicitation bid with the primer library, or will the contractor be responsible for obtaining the primer library?

A: The contractor will have to synthesize the primers library. We will provide the sequences of oligos to the contractor.

2. Q: Regarding the purified DNA that the contractor will receive from the USDA, will it meet the quality and quantity standards of 50ul of 25ng/ul or 100 ul of 10-15 ng/ul DNA, using PicoGreen quantitation?

A: Yes, we can provide DNA of this concentration and purity (50ul of 25ng/ul).

3. Q: When I downloaded the attachment, Request for Quotation, standard form 18, I only received a single page form, the first page. In the upper right corner of the form I see that this is page 1 of 23 pages. Are there an additional 22 pages that I will also need to fill out in providing this quotation, or will I only need to complete page 1? If I will be required to complete the entire 23-page document, can you send it to me as a word file?

A: Thank you for catching that error. There are an additional 22 pages which consist of the Statement of Work, Service Contract Clauses, and Service Contract Provisions. The system that generates the SF-18 can be a bit touchy and sometimes does not print off the attachment pages (as was the case here). The attachment has been updated in beta.SAM which now includes all of the missing pages for your review.