Statement of Work GBS Sequencing_.docx

Statement of Work

The service required is the development of a 4,000 SNP (single nucleotide polymorphic) marker panel for genotyping-by-sequencing (GBS) that is specific to Gossypium hirsutum such that said panel can only be implemented on the Ion S5 XL Semiconductor Sequencer and data analyzed using the Ion Torrent analysis software package. The USDA will provide the contractor sequence data of 7,000 – 8,000 G. hirsutum SNPs. From this SNP pool, the contractor will perform all required bioinformatic analyses to select 4,000 SNP markers that can be pooled into a G. hirsutum GBS panel. The contractor will run the SNP panel on 96 genomic DNAs provided by the USDA using standard Ion Torrent platform protocols and release all data acquired to the USDA. The contractor will also provide on-site training in utilizing the SNP panel on an in-house Ion S5 XL instrument.