SOW_General_Services_Scott_genotyping.pdf
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- DNA extraction and genotyping maize Federal contract opportunity
- Solicitation number
- 1232SA25Q0204
About this file
This Statement of Work (SOW) details a contract for DNA extraction and genotyping of approximately 1000 maize varieties at 8000 marker loci. The project is for the USDA Agricultural Research Service (ARS CRIS project 5030-21000-073D), with performance occurring from 8/1/2025 to 7/31/2026 at 716 Farm House Lane in Ames, IA. The contractor will extract DNA from leaf tissue provided by researchers, ensure DNA quality, determine genotypes across the genome, and deliver marker data electronically, with two key deliverables due within 60 days of receiving tissue samples: a data file containing genotype information and quality control information. The government will own all generated data, which can be used by the performing institution to improve their services. The solicitation is a total small business set-aside under NAICS code 541380 with a small business size standard of $19,000,000, and quotes are due by 3:00 PM CDT on 07/25/2025.
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| File | Type | Posted |
|---|---|---|
| TCs Under SAT Services No Options rv1.pdf |
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Statement of Work (SOW)
Project: DNA extraction and genotyping maize
GENERAL INFORMATION
1.0 Scope of Work: The objective is to determine the genotypes of approximately 1000 maize varieties at approximately 8000 SNP or other marker loci.
2.0 Background: Maize contains more than 30,000 genes that determine the characteristics of the plant. The position of each gene is called a genetic locus (plural: loci). Different versions of a gene are called alleles and many different alleles of each gene exist in nature. The genotype of a plant is the specific combination of alleles present in each plant. We use genotype information to learn how genes function to determine the characteristics of a plant and to develop new varieties with characteristics that increase the value of the crop, such as increased yield or nutritional quality. Genotypes are determined from plant tissue samples by first extracting the DNA from the tissue and examining the sequence of the DNA at the desired SNP marker loci. There are several methods for doing this, but all require a chemical process that involves liquid handling. Tissue samples are typically processed in batches of 96 using plates with 96 wells that facilitate the liquid handling steps required for genotyping.
CONTRACTOR REQUIREMENTS
3.0 Technical Requirements/Tasks:
o Extract DNA from leaf tissue provided by the researchers.
o Ensure DNA is of sufficient quality to obtain high quality marker data.
o Determine the genotype at approximately 8000 marker loci approximately randomly distributed throughout the genome.
o Deliver marker data consisting of allele calls at each locus and quality control information to the government in electronic form.
4.0 Government Furnished:
o The government (ARS CRIS project 5030-21000-073D) will provide leaf tissue from plants to be genotyped. No contractor access will be required to government facilities.
5.0 Deliverables / Schedule:
Timely submission of deliverables is essential to successfully completing this requirement. Schedules for deliverables are specified below. All deliverables shall be prepared and submitted according to format, content, and schedule described in the SOW.
The main deliverable consists of genotype data from the tissue samples provided by the Government.
Key Deliverables Item No.
Deliverable
Objective
Due
1 Data file containing genotype information for each tissue sample.
Determine genotype at approximately 8000 marker loci from tissue samples provided.
Within 60 days of receiving tissue.
2 Quality control information.
Summarize project performance with quality control data.
Within 60 days of receiving tissue
6.0 Travel: N/A
7.0 Contractor’s Key Personnel: N/A
8.0 Security Requirements: N/A
9.0 Data Rights: All data generated will be owned by the government and will be made available to the performing institution for the purpose of improving their service.
10.0 Section 508 – Electronic and Information Technology Standards: N/A
11.0 Place of Performance: 716 Farm House Lane, Ames IA 50011
12.0 Period of Performance: 8/1/2025 to 7/31/2026
13.0 Evaluation Factors:
14.0 Technical Point of Contact: Jode Edwards, 1 (515) 294-7607, Jode.Edwards@USDA.GOV
| CONTRACTOR REQUIREMENTS |
| 3.0 Technical Requirements/Tasks: |
| 6.0 Travel: N/A |
| 7.0 Contractor’s Key Personnel: N/A |
| 8.0 Security Requirements: N/A |
| 9.0 Data Rights: All data generated will be owned by the government and will be made available to the performing institution for the purpose of improving their service. |
| 10.0 Section 508 – Electronic and Information Technology Standards: N/A |
| 11.0 Place of Performance: 716 Farm House Lane, Ames IA 50011 |
| 12.0 Period of Performance: 8/1/2025 to 7/31/2026 |
| 14.0 Technical Point of Contact: Jode Edwards, 1 (515) 294-7607, Jode.Edwards@USDA.GOV |
File details come from the government source that posted it. Updated .