Contractor RFIs and Responses.pdf

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1232SA25Q0103 - RFI Responses Solicitation Sequencing of DNA Samples USDA-ARS-NEA Wyndmoor PA
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1232SA25Q0103
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The document is a Request for Information (RFI) response addressing sequencing output requirements for a federal contract opportunity. The contractor clarifies that they are requesting a minimum of 3M total reads, specifically 1.5M reads in each direction per sample. This RFI is associated with Solicitation Number 1232SA25Q0103 for shotgun sequencing of DNA samples for metagenomic analysis of gut microbial communities, issued by the USDA Agricultural Research Service. The solicitation is a 100% Small Business Set-Aside with NAICS code 541380 and a small business size standard of $19M. Key details include a submission deadline of August 7, 2025, with offers to be sent to Theodore.blume@usda.gov, and a requirement for deliverables to be completed within 60 days after order receipt, including raw data files to be provided via secure portal download.

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Contractor RFIs and Responses

1. which sequencing output do you need?

3M total reads (1.5M reads each direction) per sample or is it 6M total reads (3M reads each direction)?”

Response – We are requesting a minimum of 3M total reads (1.5M reads each direction).

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