SOW-ATAC-Sequencing1.docx
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- ATAC and ChIP Sequencing and Analysis
- Solicitation number
- 12305B19N0085
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Statement of Work
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SERVICE STATEMENT OF WORK:
SECTION A - DESCRIPTION/SPECIFICATIONS/WORK STATEMENT
A.1 BACKGROUND
The USDA ARS AGIL, located at BARC-West, Beltsville, MD is conducting research on functional annotation of cattle gnome in rumen epithelial cells and to understand the development of rumen for the purposes of improving nutrition utilization efficiency.
| A.2 | SCOPE OF WORK. The project requires: |
| 1) ATAC-sequencing (ATAC-Seq) for 2 samples with duplicate (2X2); | |
| 2) ATAC-Seq should be optimized (ATAC-Seq optimization X 1); | |
| 3)ChIP-sequencing (ChIP-Seq) of two samples with 4 histone markers (H3K4me3, H3K27me3, H3K27ac and H3K4me1, 2X4 total 8 factorPath ChIP-seq; | |
| 4) DNA-sequencing of input/control DNA; | |
| 5) ChIP-seq with transcriptpath (CTCF ChIP-seq) (2 samples). |
A.3 PROJECT OBJECTIVE
The primary objective of this work is to evevaluate the epogenomic regulation induced by nutrition element (butyrate)which is the major energy resource of cattle. The result datasets are also important for the functional annotation of cattle genome, especially the functional genomic elements that related to rumen development.
| A.4 | GOVERNMENT FURNISHED PROPERTY AND TASKS |
| Provide tissue/samples within 5 working days after notice of award and shall be responsible for all associated shipping cost for the samples sent to the Contractor. |
A.5 THE CONTRACTOR SHALL PERFORM THESE TASKS:
The Contractor shall provide preparation of chromatin samples and sonication; perform ChIP with ChIP-qualified antibodies; construct ChIP-seq libraries; perform Next-generation sequencing and finally analyze and deliver the data. Please fill out the chart below and include with your response.
A.6 SCHEDULE OF ITEMS
| Product code |
| Product |
| Description |
| Units |
| Unit Price |
| % Discount |
| Discounted |
price total
ATAC-Seq Optimization
Factor Path™ ChIP-Seq
Input-Seq
| Tissue/Primary Cells Add-on |
| Cow Rumin tissue add-on TranscriptionPath |
| 4 |
| Tissue/Primary Cells Add-on |
| Cow Rumin tissue add-on FactorPath |
| 8 |
| Tissue ATAC-Seq |
| Cattle Rumin Samples |
| 4 |
TranscriptionPath™ ChIP-Seq
GRAND
TOTAL
A.7 SECURITY REQUIREMENTS:Shipment of the samples will follow the special requirements of the samples packaged with dry ice.
A.8 PLACE OF PERFORMANCE
All work by the Contractor shall be performed at the Contractor’s facility.
A.9 DELIVERY
The Contractor shall furnish these services to the USDA, ARS, AGIL, located in Beltsville MD 20705 in accordance with the terms and conditions listed in this document.
All required documentation, as specified in this document, shall be delivered between the hours of 7:30 a.m. and 3:00 p.m., Monday through Friday (excluding Federal holidays).
All shipments shall identify the purchase order number on the label.
a) DOCUMENTATION
1) Deliverables. The contract will be considered complete upon receipt of a final report documenting the ATAC-sequencing,ChIP sequencing, and performance of Next-generation sequencing and finally analyze and deliver the data. All documentation shall identify the purchase order number on the label. All reports should be sent to TBD.
2) Reporting schedule:
The final report should be sent to TBD within 90 days after completion of the required testing.
A.10 PACKING AND SHIPPING
The Contractor shall be responsible for shipping the items. The Contractor shall pack each item for shipment in a new container in such a manner that will ensure acceptance by common carrier and safe delivery at the destination. Containers and closures shall comply with the Interstate Commerce Regulation, Uniform Freight Classification Rules, or regulation of other carriers as applicable to the mode of transportation.
The items to be furnished hereunder shall be labeled with the purchase order number and delivered, all transportation charges paid by the Contractor in accordance with the F.O.B. Destination.
A.11 PERIOD OF PERFORMANCE: June 01, 2019-August 31, 2019.
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