1118070 Sources sought specs.pdf

Project Microscopes

1.0 Scope of Work

Purchase of microscopes and associated accessories for IMMDL

2.0 Background

The Insect Management and Molecular Diagnostics Laboratory (IMMDL) is responsible for developing and validating technologies used to detect and diagnose plant pests. The focus of the laboratory’s diagnostic work is on insects that cause damage directly to plants or that serve as vectors of plant disease, but molecular biological methods are also used to support production of beneficial insects through detection of insect pathogens in rearing populations. This laboratory also identifies, develops, and provides technology transfer of a wide range of scientific methods to PPQ and State departments of agriculture, geographic information systems, biological control, area-wide pest management, and sterile insect technology (SIT) support for the Mexican fruit fly eradication program. In addition, the laboratory leads the agency’s Unmanned Aircraft Systems (UAS) initiative to develop methods for the successful incorporation of new UAS technologies into PPQ programs.

3.0 Technical Requirements

The contractor must provide items matching the described requirements below, as well as shipping of these items to the designated location in Section 5 of this document.

Fluorescent microscope:

Includes multiple sources of illumination (LED and UV) Interpupillary distance range of roughly 51-77mm Observation angle = 30° Compatible with multiple filters that modify the UV spectra to emit optimal wavelengths to visualize photoproteins, such as GFP, BFP, DsRED and mCherry Trinocular tube that supports live specimen viewing (100% switch capability between tube and eyepieces) Required accessories:

Optics carrier Minimum magnification range: 7.3x-120x (1.0x objective, 10x eyepieces) Click stop zoom settings Separate illumination beam path for fluorescence Minimum of four position fluorescence filter turrets Includes magnification, fluorescence filter, and iris diaphragm functions Apochromatic corrected optics Parfocal plan-apochromat objectives in at least four magnifications between 0.6x-3x Compatible objective threading

LED light source Generates white light output (390-680nm) through the combination of multiple solid state light outputs Mercury-free Supports fluorescence and stereo microscopy applications As maintenance-free as possible Extended lifetime while maintaining lamp quality Can be triggered to synchronize illumination with camera exposure and minimize photobleaching

Filter sets Berkeley Packet Filter (BFP) minimums: excitation 405nm at 20x; emission 460nm at 50x

Green Fluorescent Protein (GFP) minimums: excitation 470nm at 40x; emission 525nm at 50x mCherry Fluorescent Protein minimums: excitation 560nm at 40x; emission 630 at 75x

LED Spotlight Illuminator LEDs at the end of 400+ mm flexible arms (minimum of 2 arms) Microscope provides power through illuminator’s integrated cable(s) Control via microscope software and physical control keypad

Microscope Camera Complementary metal oxide semiconductor (CMOS) image sensors Maximum pixel size 2.4 µm x 2.4 µm Minimum imaging size: 3072 x 2048 pixels RGB color filter Color depth: 3x12 bits Maximum exposure time: 1 second Minimum exposure time: 1 millisecond or less Minimum frames per second video: 15 Minimum dynamic range: 72dB Gain 1x-251x Compatible with Windows operating system Includes appropriate mounting hardware for microscope provided Includes instruction manual or equivalent

Stereomicroscope:

Maximum dimensions: 350x300x25 mm AntiShock base support/feet Light base with stage insert: roughly 120 mm Modular design to support repositioning of controls, change in platform size, etc.

Required accessories:

Optics carrier Minimum magnification range: 6.3x -40x (1x objective, 10x eyepieces) Parfocal optics Click stop zoom settings Suitable for achromatic, plan or plan-apo objectives Compatible threading for use with provided microscope objectives ESD safe

LED Spotlight Illuminator LEDs at the end of 400+ mm flexible arms (minimum of 2 arms) Microscope provides power through illuminator’s integrated cable(s) Control via microscope software and physical control keypad

Transmitted light microscope:

integrated daylight LED Height adjustability of focus knobs Fixed 5-fold revolving nosepiece for brightfield objectives Stage carrier and central condenser holder for vertical adjustment Allows operation of the clamp screw by right- and left-hand with adjustable height stop Includes focus drive coarse and fine with focus stop and focus knob coarse Auxiliary lens for objective magnification minimum: 2.5x Universal condenser which allows for dry and immersion observation, bright field, optional light rings for darkfield and phase contrast, and an empty 5-position condenser disc

Ergonomic stage that provides right- or left-hand operation, a travel range of at least 76mm x 25 mm, and an incorporated vernier scale

Stage plate with ultrahard ceramic surface Slide holder for one-handed exchange Rubber x/y control Required accessories:

Oil immersion objectives Minimum magnifications: 4x, 10x, 20x, 40x, and 100x

Microscope Camera Digital color camera system CMOS sensor (1/2.3) Compatible with 4k HDMI monitor for high-definition live image (4k, 60fps, HDR) JPG quality minimum: 12 MP Full HD MJPG Video recording with 30 fps Allow for camera control via PC (Windows) using included software, via smartphone / tablet by

Mobile App (connection to Ethernet or Wifi is required) or via USB mouse (not included) Allow capture of images directly to USB Stick or send images via E-Mail Compatible with OS: Win 10Pro/ Win 11 / iOS / Android Minimum cables as applicable: HDMI, USB C-A, external power supply (without power cord), USB stick

Imaging module Mountable between the microscope stand and tube Allows for switchable beam splitting 50%:50% and 0%:100% (i.e. 100% light to the camera) Integrated 0,5x C-mount that can be easily centered Must allow rotation to side position

Computer Monitor Flat screen At least 4K HDR/QHD resolutions Between 26”-35” unit size Energy Star certified Response time maximum: 5 ms Minimum frame rate: 60 Hz Windows OS compatible USB-A ports minimum: 1 HDMI ports minimum: 1 Includes stand, and HDMI and Power cables

4.0 Pricing Schedule

Total price for each line must include all required items and incidentals per Section 3.0 of this document.

Line Number Description Quantity Price 0001 Fluorescent microscope & accessories 1 0002 Stereomicroscope & accessories 1 0003 Transmitted light microscope & accessories 1 0004 Shipping 1