1 - Bill of Material for RFQ-NIAID-23-2180055.xlsx

Sheet1

By submitting a quote in response to this solicitation, vendor is accepting the terms and conditions listed in this solicitation .

Microlens based Nipkow spinning disk multi-point confocal with:

a. Borealis Illumination (Patented). An optical technique which ensures optimal illumination uniformity to give best results when capturing tiled and stitched images of large samples, minimising objective dependent dark edge effects at field boundaries to produce a seamless single large image. This also provides highest accuracy for cross-field analysis.

b. Microlenses improve light throughput resulting in the use of lower power, more costeffective lasers, and reduced lifetime costs.

Three imaging modalities of microlens-based spinning-disk confocal, widefield epifluorescence and brightfield in a single benchtop device, and where confocal and widefield modalities utilise the same excitation light source.

• Differential Phase Contrast is a brightfield contrast method developed to help visualise samples with little or no natural contrast. A multi-angular illumination method which produces a 3D-like surface contrast of the sample. This method can be used with plastic vessels, unlike Differential Interference Contrast (DIC) which relies on polarisation which plastic disrupts.

All hardware elements (including lasers) must be incorporated into a single unit

Dimensions cannot exceed: footprint of W x D x H: 505mm x 633mm x 448mm o be able to fit the existing bench space in the lab.

Focus Seek & Lock technology.

• Focus Seek quickly (within 15 to 20 seconds), single software button click, finds the sample focus for the user.

• Focus Lock uses the same mechanism to then lock on to the sample to maintain long term focus during long experiments such as time-lapse and multi-field imaging.

Includes built-in vibration isolation solution. This ensures image stability even when other devices (e.g. mini vortex mixers or centrifuges) are on the same bench.

Includes a joystick for x,y stage movement and focus control. It is much easier and quicker to find and navigate the sample with a joystick rather than other software driven solutions.

Light-tight, compared to other confocal technology that is mounted on the side port of a conventional microscope. Being light-tight there is no need for a specialised dark room or addition of an external black-box which restricts access to the sample on conventional microscope-based confocals.

Compared to point-scanning confocal technology. Faster frame-rates at a like-for-like field size and x,y pixel number (e.g. 2048 x 2000 pixels and field size diagonal of 18.4mm). BC43 is typically 6x faster, often considerably more depending on the imaging experiment and alternative confocal technology being used.

Compared to other point-scanning confocal technology the BC43 has a higher quantum efficiency (QE) detector (peak 82%) compared to ~42%. A higher QE result in:

a. Lower fluorescence signals being detected.

b. Shorter frame rates possible for imaging fast dynamic processes

c. Lower intensity illumination to protect from phototoxicity and photobleaching.

Compared to other spinning disk confocal technology. Larger inter-pinhole spacing (>500um) delivers higher background rejection which results in higher contrast images in thicker samples and the ability to image deeper and maintain higher image quality.

Objective magnification range of between 2x and 100x.

Benchtop Confocal Microscope. Including 2x objective and joystick for motorized sample positioning. Equipped with: Brightfield and Differential Phase Contrast transmitted white light illumination, widefield epifluorescence for low-light imaging and patented Borealis™-illuminated multipoint confocal for fast high-contrast 2D & 3D imaging. Includes built-in vibration management, dual function Focus Seek and Lock™. Excitation lines 405nm, 488nm, 561nm & 638nm, and emission filters for commonly used fluorophores such as DAPI, GFP, mCherry, CY5. 4.1 MP (6.5μm pixel, 16-bit) sCMOS camera up to 82% QE. 18.4 mm field of view. Workstation: 6 Core, 18M Cache, 3.3GHz up to 4.8GHz; 64GB RAM; 512GB SSD Boot drive; 8GB Graphics card, 2TB Data storage; Windows 10 operating system.

Fusion BC43 acquisition software for multi-dimensional experiments in 2D, 3D, time series, multi-field, multi-well and large area montage with image stitching. Renders 3D images in real-time as a volume.

52.211-6 Brand Name or Equal.

As prescribed in 11.107(a), insert the following provision:

Brand Name or Equal (Aug 1999)

(a) If an item in this solicitation is identified as "brand name or equal," the purchase description reflects the characteristics and level of quality that will satisfy the Government’s needs. The salient physical, functional, or performance characteristics that "equal" products must meet are specified in the solicitation.

(b) To be considered for award, offers of "equal" products, including "equal" products of the brand name manufacturer, must-

(1) Meet the salient physical, functional, or performance characteristic specified in this solicitation;

(2) Clearly identify the item by-

(i) Brand name, if any; and

(ii) Make or model number;

(3) Include descriptive literature such as illustrations, drawings, or a clear reference to previously furnished descriptive data or information available to the Contracting Officer; and

(4) Clearly describe any modifications the offeror plans to make in a product to make it conform to the solicitation requirements. Mark any descriptive material to clearly show the modifications.

(c) The Contracting Officer will evaluate "equal" products on the basis of information furnished by the offeror or identified in the offer and reasonably available to the Contracting Officer. The Contracting Officer is not responsible for locating or obtaining any information not identified in the offer.

(d) Unless the offeror clearly indicates in its offer that the product being offered is an "equal" product, the offeror shall provide the brand name product referenced in the solicitation.

(End of provision) https://www.acquisition.gov/far/11.107