IVD EIT: a nonanimal alternative method for the… (SB1ES038803) – Project Grant to Lebrun Labs LLC

Abstract: Currently, the draize live rabbit eye test is the benchmark to distinguish consumer products and materials that are safe from those that are hazardous and can cause mild, moderate, or severe ocular damage. However, new laws are currently being, or are planned to be, implemented in california, the united states, and the european union to BAN the use of animals for ocular irritation testing. Despite this fact, there is currently no single nonanimal test or combination of nonanimal tests that can accurately classify all levels of ocular irritants and corrosives. IT is of particular concern that all nonanimal tests do not satisfy the regulatory testing needs required to label product that eye protection is not/is required. This target classification represents a critical unmet need for correctly informing and advising consumers and manufacturers about product safety. We have recently developed, patented and tested a new method, the in vitro depth of injury eye irritation test (IVD EIT), to detect all classes of irritants with high sensitivity and specificity. The purpose of this application is to further develop the IVD EIT with the long-term goal of providing a service and/or kit for the accurate classification of ocular irritants to meet this unmet need for a nonanimal ocular irritation test to be used to label product and chemicals and hence ensure safe handling practices. 1

IVD EIT: a nonanimal alternative method for the detection of ocular irritants