Timstof flex maldi-2 for spatial molecular imaging

Ended Project Grant Awarded Last action

Award ID
S10OD038264
Recipient
Awarding agency
Obligated
$1,759,412

Award facts

Assistance listing
93.351 Research Infrastructure Programs
Completion date
Funding agency
Office of the Director National Institutes of Health, Department of Health and Human Services
Place of performance
California, USA

Award details come from USAspending.gov.

Description

As the agency reported it.

Abstract
This shared instrumentation proposal seeks funding for the purchase of a bruker 'timstof flex MALDI-2 for spatial molecular imaging' including a bundled HTX M3+ sprayer. This new mass spectrometry (MS) platform will facilitate ongoing and future scientific research to spatially resolve molecular signatures, even in a multi-omic workflow monitoring proteins, glycans, lipids, and metabolites all within the same tissue down to a spatial resolution of ~5-10 ΜM. All costs will be accounted for with this proposal. We propose to operate this integrated timstof flex MALDI-2 mass spectrometry system exclusively for spatially resolved molecular imaging of human and animal tissues and organoids as part of large group of 18 independent principal investigators from the buck institute and seven other institutions or universities. These researchers bring together 19 NIH-funded grants (including P01, U54, U01, R01, P30, UG3 activity codes) and one R01 supplement, all of which have significant needs for state-of-the-art mass spectrometry for spatially resolved proteomics, metabolomics, lipidomics and glycan analysis - even in a multi-omic systems approach. The most critical features of this new instrumentation will be the 1) second post-ionization laser (MALDI-2) to greatly reduce noise and to improve ionization and sensitivity substantially, 2) full MS imaging workflow solutions with workflows using the HTX M3+ sprayer bundle for sample preparation and matrix application required for MS imaging, 3) high spatial resolution to reveal small distinct architectural features in tissues, 4) dual tims ION mobility for reduction of complexity, separation of isobaric features and increased sensitivity and selectivity for improved MS imaging, 5) lipid and metabolite MS imaging using ION mobility cross section (CCS) measurements as additional identifier (CCS) using the metaboscape databases and scils (combination of M/Z and CCS in spectral libraries and databases), and 6) true multi-omic approach for MS imaging. Overall, the acquisition of this instrument to the mass spectrometry core at the buck institute for research on aging will provide unique and novel features that will bring innovation and totally novel capabilities (spatial molecular MS imaging) for our 18 proposed user projects that are examining key issues of human health and health span, including the this innovative mass spectrometry molecular imaging technology to study the biology of aging including differential aging of specific organs and to investigate age-related diseases, including alzheimer's and huntington's disease, a parkinsonism, osteoarthritis, kidney diseases and metabolic disorders.

Transactions

Action Obligated Date
Amendment $0
Original award $1,759,412

Quick answers

Who received award S10OD038264?

S10OD038264 was awarded to Buck Institute For Research On Aging by Aggregate Financial Assistance Data (National Institutes of Health) on April 1, 2025.

How much is award S10OD038264 worth?

$1,759,412 has been obligated on S10OD038264.

Which agency awarded S10OD038264?

Aggregate Financial Assistance Data (National Institutes of Health) awarded S10OD038264, with funding from Office of the Director (National Institutes of Health).

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