This $2,576,654 Project Grant awarded by the National Institute of Mental Health (NIMH) under the Mental Health Research Grants program (CFDA 93.242) will support the development of a cloud-based software platform and advanced computational pipelines to harmonize and integrate spatial transcriptomics data for the construction of a spatially resolved brain atlas.
Key objectives of the 3-year project include: 1) Creating a cloud-based platform to standardize the analysis and visualization of...
This three-year, $4.485 million project grant from the National Institute of Mental Health will develop protocols and workflows for a new imaging approach called Serial-Section Parallel Immunohistochemistry/Fluorescence In Situ Hybridization (SPIFISH). SPIFISH aims to enable highly multiplexed, high-resolution imaging of endogenous molecules in brain tissue. The approach physically subdivides tissue specimens into ultra-thin sections, two orders of magnitude smaller than a neuronal cell body,...
This Project Grant award from the National Institutes of Health (NIH) Trans-NIH Research Support program (CFDA 93.310) provides $1,271,280 to the Allen Institute to develop two new "exa-scale" tissue readout methods that will dramatically improve the throughput and resolution of molecular imaging across large mammalian brain tissue samples. The first platform will enable highly multiplexed mapping of individual proteins at the molecular level across entire brains, while the second...
The National Institute of Mental Health awarded a three-year Project Grant totaling $3,618,976 to the University of California, Los Angeles under the Mental Health Research Grants program (CFDA 93.242) to develop in situ single-cell multi-omic and morphological profiling methods for mammalian brains. Specifically, the university will work to design photonic-indexing sequencing strategies to spatially barcode tissue with single-cell resolution, integrating techniques such as PI-ATAC-seq and...
This $538,523 Project Grant awarded by the National Institute of Neurological Disorders and Stroke (NINDS) through the Extramural Research Programs in the Neurosciences and Neurological Disorders (CFDA 93.853) supports the development of an automated imaging and analysis platform to study gliogenesis, the production of glial cells, in the mouse forebrain.
The key products and services to be delivered include:
Optimizing a pipeline for simultaneous and automated detection and classification...
This three-year, $4.45 million project grant from the National Institutes of Health's Trans-NIH Research Support program will fund research into unraveling the developmental relationship between gene expression and long-range cortical projections in neurons. The Allen Institute will receive the funding to improve the resolution and scale of in situ sequencing-based neuroanatomical approaches. This will generate an unprecedented view of how gene expression correlates with and influences the...
This $7.6 million project grant from the National Institute of Mental Health, part of the Department of Health and Human Services, will fund research under the Mental Health Research Grants program (CFDA 93.242). Baylor College of Medicine will lead simultaneous high-throughput functional, transcriptomic, and connectivity profiling of neurons in the mouse visual cortex. The research aims to characterize the complex functional properties of neurons using in vivo two-photon imaging combined...
This $21.4 million cooperative agreement from the National Institute of Mental Health will fund research to identify gene regulatory elements that can target and manipulate specific neuronal cell types and brain circuits across species, including in human organoids. Awarded on September 1, 2022 to the Broad Institute, Inc. with completion by August 31, 2025, the funding supports the Mental Health Research Grants program.
The Broad Institute and collaborators at Harvard University will profile...
This federal Project Grant award from the National Institute of Mental Health (NIMH), under the Mental Health Research Grants program (CFDA 93.242), provides $2,415,243 to The Broad Institute, Inc. to develop a scalable, cloud-based framework for multi-modal mapping across single neuron omics, morphology, and electrophysiology. The framework aims to enable integrative, data-driven characterization of brain cell types, leveraging federated Brain Initiative resources and community engagement....
This $1.8 million project grant from the National Institutes of Health's National Institute of Mental Health will fund the development of high-throughput methods for measuring synaptic connectivity at single-cell resolution in the cerebral cortex. Over a three-year period from September 2022 through August 2025, The Broad Institute, Inc. will pursue viral, functional, and molecular approaches to map local cortical circuitry. These include optimizing rabies-based trans-synaptic tracing for...