Improving extraction success of ffpe samples with automated and reliable microfluidic sample preparation
Active Project Grant Awarded Last action
- Award ID
- R44GM148126
- Recipient
-
Redbud Labs, Inc. UEI KJGXVMFV9U14
- Awarding agency
-
National Institute of General Medical Sciences
National Institutes of Health, Department of Health and Human Services
- Obligated
- $5,111,574
Award facts
- Assistance listing
- 93.859 Biomedical Research and Research Training
- Completion date 86% complete
- Originally
- Place of performance
- Research Triangle Park, Durham, NC, USA
Award details come from USAspending.gov.
Description
- Abstract
- Genomic sequencing data is suitable for informing clinical decisions and advancing precision medicine. While fresh-frozen tissues are the gold standard for next-generation sequencing, only fixed paraffin-embedded (FFPE) tissue blocks are widely available in clinical workflows. The fixation process enables a readily available, cost-effective resource repository with preserved morphology and cellular data. However, extraction after fixation frequently yields low quantities of degraded nucleic acids (NAS). The quantity and quality of nucleic acids can be improved during extraction, however current workflows remain highly dependent on manual methods that are labor-intensive, time-consuming, and vary in quality by operator skill. Extracting nucleic acids from FFPE samples requires robust protocols with the ability to maintain consistent performance. The translation from manual to reliable automated methods will be crucial to advancing the field. In phase I, we demonstrated proof-of-concept for extracting NAS from FFPE using our microfluidic modules in combination with well-established sample prep chemistries. In phase II, we will fully automate the fluid processing from module to module using our sample prep automation platform, naxtract.
Transactions
| Action | Obligated | Date |
|---|---|---|
| Amendment | $0 | |
| Amendment | $50,000 | |
| Amendment | $50,000 | |
| Amendment | $1,261,092 | |
| Amendment | $1,261,092 | |
| Amendment | $0 | |
| Amendment | -$11,574 | |
| Amendment | $1,250,482 | |
| Original award | $1,250,482 |
Quick answers
Who received award R44GM148126?
R44GM148126 was awarded to Redbud Labs, Inc. by National Institute of General Medical Sciences (National Institutes of Health) on September 16, 2022.
How much is award R44GM148126 worth?
$5,111,574 has been obligated on R44GM148126.
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