Project Grant R44AI124933

This Project Grant award from the National Human Genome Research Institute (NHGRI) under the Human Genome Research program (CFDA 93.172) provides $215,088 to Directseq Biosciences, Inc. to develop and test an "Exhaustive De Novo Sequencing of Every RNA in a Sample by a Layer-by-Layer Mass Spectrometry Ladder Intensity Approach." The project aims to create a comprehensive RNA sequencing and modification profiling method that can identify and sequence all RNA species in a sample...

This National Science Foundation (NSF) Cooperative Agreement award, funded under the NSF Technology, Innovation, and Partnerships (TIP) program (CFDA 47.084), provides $1,250,000 to Micropure Genomics Inc., a minority-owned small business, to develop an innovative automated platform for preparing high-quality DNA samples for long-read sequencing applications. The project aims to address critical barriers in genomic sequencing by advancing a novel technology for DNA sample preparation. Key...

Micropure Genomics Inc. received a $274,199 Project Grant award from the National Science Foundation to advance methods for rapid, end-to-end sample preparation for sequencing applications. Under the NSF Technology, Innovation, and Partnerships program (CFDA 47.084), the company will develop an automated process relying on electro-hydrodynamic trapping to isolate genomic material like DNA or RNA from cells in a flow cartridge. The process aims to significantly reduce preparation time while...

Triton Systems, Inc. received a $249,712 Project Grant award from the Department of Energy Office of Science on February 22, 2021 to develop a fast, scalable, automated, one-pot process for assembly of DNA oligonucleotides into large gene sequences. The work will be performed in Chelmsford, Massachusetts through February 21, 2022. The award is being made under the Office of Science Financial Assistance Program (CFDA #81.049), which supports basic research to deliver scientific discoveries and...

This Project Grant award from the National Institutes of Health's National Human Genome Research Institute provided $768,678 to Molecular Assemblies, Inc. to develop a fully enzymatic oligonucleotide synthesis cycle using engineered template-independent polymerases and a novel phosphate dNTP blocking group. The award supports the goals of the Human Genome Research program (CFDA 93.172) to accelerate genome research and its application to human health. Specifically, the awardee will engineer...

This Project Grant award from the National Institutes of Health's National Human Genome Research Institute (CFDA 93.172 - Human Genome Research) provides $406,500 to Directseq Biosciences, Inc. to advance their next-generation mass spectrometry-based sequencing (NGMS-SEQ) methods for high-throughput direct sequencing and quantitative mapping of RNA modifications. The key objectives are to: 1) enhance NGMS-SEQ techniques to enable direct sequencing of various tRNA samples, including...

This $256,000 National Science Foundation Engineering grant supports the development of an easy RNA adenylation method for deep sequencing of picogram RNA samples with high resolution. Specifically, Ezrabio Inc. will develop a versatile and cost-effective library construction technology that enables protein translation monitoring on a variety of biological samples. The proposed technology relies on a novel enzyme system capable of 5' adenylation and 3' polyadenylation of RNA fragments to...

This Project Grant award, valued at $345,285, was provided by the National Institute of General Medical Sciences (NIGMS) under the Biomedical Research and Research Training program (CFDA 93.859). The funding will support the development of a higher order multiplexing method for digital PCR (dPCR) systems, known as the ZIP-MELT Multiplex Probe Technology (ZIP-MELT MPT). This innovative probe-based approach aims to expand the capacity of dPCR by enabling the detection and quantification of...

The National Institute of General Medical Sciences (NIGMS) awarded a $305,892 Project Grant (CFDA 93.859 - Biomedical Research and Research Training) to Dotquant LLC, a for-profit biotechnology research and development company, to develop a new in vitro transcription kit for high-yield synthesis of chemically modified RNA. This innovation aims to drastically improve the affordability of current and future RNA drugs by combining the benefits of wild-type T7 RNA polymerase's high efficiency and...

This Project Grant award from the National Science Foundation (NSF) under the Technology, Innovation, and Partnerships (TIP) program (CFDA 47.084) supports the development of an autonomous, miniaturized system for DNA extraction and preparation for sequencing. The $275,000 award to Anyglabs, Inc. will fund the creation of a portable, automated device capable of high-throughput DNA processing in space and other austere environments. The goal is to democratize access to advanced molecular...