Project Grant R43NS125749
- This $1,417,500 Project Grant awarded by the National Institutes of Health (NIH) under the Trans-NIH Research Support program (CFDA 93.310) funds the development of "Electrified Cryo-EM", a novel tool that can kinetically trap biological systems in their metastable, non-equilibrium states. The research aims to unravel the structural changes in neurons during an action potential across multiple length scales, from voltage-gated channel proteins to the synaptic junction. Specifically,...
- This NSF Engineering Program award of $605,320 to North Carolina State University (NC State) supports the development of a flexible, FMRI-compatible neural probe with multi-modal sensing and modulation capabilities. The novel probe is designed to advance understanding of neural circuit dynamics and enable closed-loop neuromodulation for treating neurological disorders. Key product elements include: Flexible, multi-shank neural probe fabricated using polymeric materials to enable deep brain...
- This Project Grant award, valued at $481,250.00 and provided by the National Institute of Neurological Disorders and Stroke (NINDS) under the Extramural Research Programs in the Neurosciences and Neurological Disorders (CFDA 93.853) program, aims to develop a synthetic biological system to manipulate subcellular RNAs and proteins in growth cones or synapses of subtype- and context-specific neurons. The goal is to enable unique forms of subcellular functional investigation in subtype- and...
- This Small Business Innovation Research (SBIR) Phase I project, awarded by the National Science Foundation (NSF) under the TIP (Technology, Innovation, and Partnerships) program (CFDA 47.084), aims to develop a novel, multifunctional neural probe for advancing neuroscience research. The $254,456 grant awarded to Neurobionics Inc. on August 15, 2024, will fund the integration of electrical, optical, and chemical capabilities into a single implantable device. This versatile tool will enable...
- This National Science Foundation (NSF) Engineering (CFDA 47.041) Project Grant award to Carnegie Mellon University (CMU) provides $500,000 in funding from July 1, 2024 to June 30, 2027 to develop a new computational framework for predicting neuron growth and transport regulation in biological neural circuits (BNCs). The project aims to advance knowledge of the fundamental mechanisms of neural growth, material transport regulation, and circuit dynamics through three specific objectives: (1)...
- The National Institutes of Health (NIH) awarded a $1,385,000 Project Grant under the Trans-NIH Research Support program (CFDA 93.310) to the Allen Institute, a non-profit research organization, to develop novel techniques for measuring the input-output operations of individual neurons in the mouse cortex. The project aims to overcome challenges in understanding how neurons in the mammalian brain transform their synaptic inputs into outputs, which is critical for designing effective brain-machine...
- The National Science Foundation (NSF) awarded a $300,000 Project Grant under its Biological Sciences program (CFDA 47.074) to the Georgia TECH Research Corporation for the "TOOLS4CELLS: EAGER: A MOLECULAR PURSUIT FOR THE ENGRAM: MICROFLUIDIC TEMPORAL TRANSCRIPTOMICS FOR SINGLE CELL LEARNING" project. This 2-year effort aims to develop innovative microfluidic tools and workflows to investigate the role of non-coding RNA in learning and memory storage in single-celled organisms. Key...
- Grant Award Summary Cold Spring Harbor Laboratory received a $1.41 million Project Grant from the National Institute of Neurological Disorders and Stroke (NINDS) under the Extramural Research Programs in the Neurosciences and Neurological Disorders program (CFDA 93.853), effective August 1, 2025 through April 30, 2030. The award supports structural and functional research into calcium homeostasis modulator (CALHM) proteins, which are large-pore ion channels implicated in neurological disorders...
- The National Science Foundation (NSF) Engineering Directorate awarded a $564,510 Project Grant to the University of Massachusetts (UMass) to develop an engineered neural organoid system with improved brain regionalization and integrated mesh electrodes for enhanced monitoring and stimulation capabilities. The key objectives of this 3-year grant project are to: 1) Assemble regionalized neural organoids that enable thalamus-subpallium-cortex projections by stacking sliced organoids, 2) Innervate...
- This Project Grant awarded by the National Science Foundation (CFDA 47.041 - Engineering) to Cleveland State University (CSU) provides $500,000 in funding from January 1, 2025 to December 31, 2027 to develop bioprinted tissue scaffolds with Schwann cell density gradients and electrical conductivity for peripheral nerve regeneration. The goal is to understand how Schwann cell density gradients and electrical properties within the scaffolds can enhance the regeneration of injured peripheral...
REAL TIME NEURON SIMULATION FOR EXPERIMENTAL APPLICATIONS - THE GOAL OF THIS PROPOSAL IS TO COMBINE THE POWER OF THE NEURON MATHEMATICAL MODELING SOFTWARE WITH THE CYBERCYTE "PLUG AND PLAY" DYNAMIC CLAMP SYSTEM. OUR PRODUCT WILL ENABLE ALL NEURONAL ELECTROPHYSIOLOGISTS TO BE ABLE TO PERFORM SOPHISTICATED NEURON MODEL BASED DYNAMIC CLAMP EXPERIMENTS, WITHOUT ANY REQUIREMENT FOR PROGRAMMING, ENGINEERING, OR MATHEMATICAL MODELING SKILLS. OUR PRODUCT IS AN INTEGRATED PACKAGE OF HARDWARE AND SOFTWARE SPECIFICALLY FOR NEUROSCIENCE APPLICATIONS, FOCUSING ON THE SPECIFIC STABILITY AND RELIABILITY NEEDED FOR ROUTINE NEURONAL ELECTROPHYSIOLOGY AND THE LARGE ARRAY OF ION CHANNELS FOUND IN THE NERVOUS SYSTEM. THE FOUR AIMS OF THIS PROJECT ARE: AIM 1. IMPLEMENT AND TEST ELECTRONIC EXPRESSION MODE. IN THIS AIM, THE PATCH CLAMP AMPLIFIER IS USED IN CURRENT CLAMP MODE TO RUN CELL-BASED ACTION POTENTIALS FROM LIVE CELLS, AUGMENTED WITH COMPUTER MODELS OF SPECIFIC CHANNELS. ARTIFICIAL ION CHANNELS GENERATED BY COMPUTER MODELS ARE USED TO INJECT AN EQUIVALENT CURRENT TO MIMIC THE EFFECTS OF CHANNEL MUTATIONS, GAIN OF FUNCTION, STATE DEPENDENT DRUG BINDING ETC., TO REVEAL THEIR MECHANISMS OF ACTION ON THE EXCITABILITY OF REAL NEURONS. THIS CAN BE THOUGHT OF AS AN INEXPENSIVE "SHORT CUT" TO THE PAINSTAKING PROCESS OF GENERATING GENETIC MODELS OF ION CHANNELS AND OTHER ELECTROPHYSIOLOGICAL MODELS. AIM 2. IMPLEMENT AND TEST SYNTHETIC CELL MODE. IN SYNTHETIC CELL MODE, ALL OF THE COMPONENT CURRENTS, EXCEPT FOR THE ONE OF INTEREST, ARE MODELLED, ALONG WITH MEMBRANE ACTION POTENTIALS. THE CURRENT OF INTEREST IS THEN GENERATED IN, FOR EXAMPLE, AN HEK CELL EXPRESSING THE CHANNEL OF INTEREST AND CONTROLLED BY A VOLTAGE-CLAMP AMPLIFIER. THE COMMAND INPUT TO THE VOLTAGE CLAMP IS THE SIMULATED ACTION POTENTIAL FROM THE DYNAMIC CLAMP SYSTEM WITH THE SYNTHETIC CELL. FOR EXAMPLE, REAL DRUGS CAN BE ADDED TO THE CLONED CHANNEL OF INTEREST OR THE CONSEQUENCES OF A REAL KINETIC MUTATION CAN BE ANALYZED. AIM 3. IMPLEMENT AND DEMONSTRATE CELL COUPLING MODE. CELL COUPLING MODE WAS ARGUABLY THE FIRST FORM OF DYNAMIC CLAMP INVENTED. ORIGINALLY IT USED ANALOG CIRCUITRY TO MIMIC GAP JUNCTIONAL RESISTANCE BETWEEN CELLS. WITH NEURON, WE CAN IMPLEMENT COMPLEX FORMS OF CELL TO CELL COUPLING, INCLUDING SYNAPTIC TRANSMISSION AND INTERNEURONS. AIM 4. IMPLEMENT AND TEST DIAGNOSTICS AND EXPERIMENTAL SAFEGUARDS. A MAJOR LIMITATION OF DYNAMIC CLAMP APPLICATIONS IN RESEARCH & DEVELOPMENT, PARTICULARLY IN COMMERCIAL APPLICATIONS, IS THE DIFFICULTY IN MAINTAINING QUALITY CONTROL. THIS AIM HELPS AUTOMATE THE PROCESS OF QUALITY CONTROL TO MAKE THE SYSTEM ACCESSIBLE TO NON- SPECIALIST USERS. COMPLETION OF THESE AIMS WILL RESULT IN A COMMERCIAL ADVANCED DYNAMIC CLAMP SYSTEM WITH AN INTERFACE TO NEURON, WHICH IS POWERFUL, RELIABLE, BUT PLUG AND PLAY TO INSTALL, AND SIMPLE TO USE.
Mod # | Description | ReasonForModification | Federal Obligation | Date |
|---|---|---|---|---|
| Not listed | $0 | 12/2/24 | ||
| Not listed | $256.6k | 7/15/22 | ||
| Not listed | $256.6k | 7/15/22 |