Project Grant R43GM163512
- The National Institute of General Medical Sciences awarded Monod Bio, Inc. $377,260 for design and development of rapid, quantitative biosensors using computational protein design, with an award date of August 1, 2026. Monod Bio is advancing its NovoLISA technology to transform biosensing methods in life sciences research. The work addresses limitations of traditional biosensing approaches such as ELISAs, which require 90–270 minutes for results, involve complex procedures, demand extensive...
- The National Institute of General Medical Sciences awarded Potomac Affinity Proteins, LLC $377,602 on July 1, 2026, under the Biomedical Research and Research Training program (CFDA 93.859, Assistance Listing R43GM159468) to develop protease chain reaction (PROCR) technology for rapid, highly sensitive, and quantitative detection of biomolecules in mix-and-read assays. The recipient will engineer protease and inhibitor components to optimize catalytic turnover and detect three target...
- The National Institute of General Medical Sciences awarded Molecular Instruments, Inc. $306,788 on September 30, 2025, under the Biomedical Research and Research Training program (CFDA 93.859) to advance probe technology for ultrasensitive RNA imaging via hybridization chain reaction (HCR). The project develops and refines in situ amplification methods based on HCR mechanism to overcome multi-decade limitations in RNA fluorescence in situ hybridization (RNA-FISH). Specifically, the work...
- The National Institute of General Medical Sciences awarded the University of California, San Diego $150,104 under the Biomedical Research and Research Training program (CFDA 93.859) on March 1, 2026. The award funds de novo design of artificial haloperoxidase enzymes using machine-learning tools for protein design and structure prediction. The research investigates metalloenzyme structure-function relationships through design of vanadium- and heme-dependent haloperoxidase enzymes that oxidize...
- The National Institute of General Medical Sciences awarded $124,767 to The Regents of the University of California, San Francisco on May 1, 2026, under the Biomedical Research and Research Training program (CFDA 93.859) to advance de novo protein design for catalysis and fluorescence imaging. The research tests the hypothesis that protein dynamics, particularly enrichment of productive conformations, contribute to enhanced catalytic rates in engineered proteins. Prior work under this funding...
- The National Institute of General Medical Sciences awarded the University of Washington $2,226,244 on September 1, 2026, under the Biomedical Research and Research Training program (CFDA 93.859) to develop nanopore technology for single-molecule protein sequencing of intact proteoforms. The funded work advances unfoldase-coupled nanopore arrays to directly sequence native proteins at single-molecule resolution, detect post-translational modifications, and analyze complex proteoforms. The project...
- The National Institute of General Medical Sciences awarded Michigan Technological University $383,801 on May 1, 2026, under the Biomedical Research and Research Training program (CFDA 93.859) to develop cost-effective, scalable biosensing platforms for rapid multiplex biomarker detection in sepsis, allergy, and other complex diseases. The research focuses on molecularly imprinted polymers (MIPs)—synthetic receptors that mimic antibody recognition with superior stability and lower production...
- The National Institute of General Medical Sciences awarded the University of Washington $891,007 on June 1, 2026, under the Biomedical Research and Research Training program (CFDA 93.859) to develop multiplexed optical sensors for redox profiling in human induced pluripotent stem cell models of disease and drug response. The funded research program develops next-generation, multiplexed optical platforms for quantitative redox phenotyping and applies them to disease modeling and drug screening in...
- The National Institute of General Medical Sciences awarded President and Fellows of Harvard College $125,000 on April 1, 2026, under the Biomedical Research and Research Training program (CFDA 93.859) to develop chemical biosensors that accelerate pharmaceutical biocatalyst engineering. The project, titled "Deploying Biosensors to Accelerate Pharmaceutical Biocatalyst Development," addresses manufacturing bottlenecks in drug production by engineering bacterial transcription factors...
- The National Institute of General Medical Sciences awarded $492,931 to the Regents of the University of Minnesota on April 1, 2026, under the Biomedical Research and Research Training program (CFDA 93.859) to develop multiplexed assays of variant effect (MAVE) pipelines that integrate cellular context into synthetic protein engineering. The award funds research to advance understanding of how protein molecular traits—including folding stability and trafficking—function across different cell...
The National Institute of General Medical Sciences awarded Monod Bio, Inc. $330,955 on August 1, 2026, under the Biomedical Research and Research Training program (CFDA 93.859) to develop computational protein design methods for robust and scalable recombinant horseradish peroxidase (HRP) and poly-HRP reagents. Current HRP reagents derive from horseradish root, yielding heterogeneous mixtures of isoenzymes with variable glycosylation patterns, activity, and stability that compromise reproducibility and complicate regulatory compliance. Recombinant HRP production has faced persistent obstacles due to complex structural requirements—heme cofactor incorporation, multiple disulfide bonds, calcium ions, and glycosylation—that microbial hosts cannot readily replicate. Poly-HRP alternatives exhibit significant batch-to-batch inconsistency, a critical limitation in high-sensitivity assays. This SBIR Phase I project pursues two primary innovations: design of a stable, de novo HRP enzyme expressed efficiently in microbial systems, and development of a self-assembling HRP nanoparticle enabling precise control of enzyme stoichiometry and enhanced performance consistency. The resulting HRP mimetic will support high-quality production of HRP reagents used in diagnostics and research tools including ELISAs, Western blots, and immunohistochemistry, and will enable genetic fusions with other functional domains for more consistent diagnostic development. Performance occurs in Seattle, Washington. The period of performance runs from August 1, 2026, through July 31, 2027.
Mod # | Description | ReasonForModification | Federal Obligation | Date |
|---|---|---|---|---|
| Not listed | $331.0k | 7/23/26 |