The National Institute of General Medical Sciences (NIGMS) awarded Mitegen, LLC a $306,852 Project Grant under the Biomedical Research and Research Training program (CFDA 93.859) to develop and validate automated cryopreservation technology that can rapidly cool and warm small biological samples like oocytes and embryos. The project aims to optimize conventional convective cooling and warming processes to increase rates by 100x, eliminate ice formation, and improve reproducibility - which can enhance cryopreservation outcomes for applications in medicine, biomedical research, and assisted reproduction. Through this grant, Mitegen will develop a flexible robot-based workstation and ultra-low thermal mass sample supports to automate and accelerate the cryopreservation process. The technology will be validated through measurements of cooling/warming rates and ice formation in aqueous solutions, as well as studies of ice formation, survival, development, and transcriptomes of bovine and porcine embryos. This grant supports Mitegen's ongoing work to advance biomedical research capabilities through innovative sample preparation and cryogenic technologies.