Developing a sensitive and quantitative western blot kit
Ended Project Grant Awarded Last action
- Award ID
- R43GM142361
- Recipient
-
Dotquant LLC UEI HPN4P84J65F7
- Awarding agency
-
National Institute of General Medical Sciences
National Institutes of Health, Department of Health and Human Services
- Obligated
- $767,020
Award facts
- Assistance listing
- 93.859 Biomedical Research and Research Training
- Completion date
- Funding
-
- $510,997.74 Federal
- $256,022.00 Non-federal
- Place of performance
- Washington, USA
Award details come from USAspending.gov.
Description
- Abstract
- Western blotting is one of the most widely used bioanalytical techniques for the detection of proteins of interest in an unknown mixture such as cell/tissue homogenate or extract. This technology was invented in the late 1970S and has enabled numerous discoveries in biology and medicine in the past 40 years. Although IT is not in the spotlight anymore, western blotting has been applied at least two times more often than other competing or complementing techniques, such as mass spec, elisa, and IHC within the last 10 years. Despite this amazingly wide acceptance by the scientific community, western blotting, in general, is not a very sensitive technique and is only semi-quantitative. The lack of quantitation in western blot has raised major concerns in the scientific community on data reproducibility. Building on two breakthroughs we made in recent years, monovalent qdot probes and optically clear transfer membranes, we propose to develop the next generation western blot kits with improved sensitivity, quantification ability, multiplexing capability and flexibility in readout (standard GEL UV imager or microscopy-based particle counting). If successful, we expect this technology to quickly become a commercial product to power biology research for another 40 years. .
Transactions
| Action | Federal | Non-federal | Date |
|---|---|---|---|
| Amendment | -$1,046 | $0 | |
| Amendment | $256,022 | $0 | |
| Original award | $256,022 | $256,022 |
Quick answers
Who received award R43GM142361?
R43GM142361 was awarded to Dotquant LLC by National Institute of General Medical Sciences (National Institutes of Health) on April 1, 2021.
How much is award R43GM142361 worth?
$767,020 has been obligated on R43GM142361: $510,998 in federal funding and $256,022 in non-federal funding.
On GovTribe
See this award's full record
- Every transaction and subaward
- The recipient's other awards and teaming partners
- Similar grant awards
- Ask GovTribe AI about this award