Sample barcoding for ultra high-throughput flow cytometry
Ended Project Grant Awarded Last action
- Award ID
- R43GM140527
- Recipient
-
Lase Innovation Inc. UEI LQDAZMN14NM7
- Awarding agency
-
National Institute of General Medical Sciences
National Institutes of Health, Department of Health and Human Services
- Obligated
- $759,768
Award facts
- Assistance listing
- 93.859 Biomedical Research and Research Training
- Completion date
- Funding
-
- $506,512.00 Federal
- $253,256.00 Non-federal
- Place of performance
- Massachusetts, USA
Award details come from USAspending.gov.
Description
- Abstract
- High-throughput screens are the workhorse of the early drug discovery process. Millions of biochemical or cell-based assays are conducted to test the activity of small molecules, antibodies and owher compoxndv WO idenwif\ ³hiwv´ or drxg candidawev for fxrwher study, including pre-clinical and clinical studies. However, the most used technologies for high-throughput screening, such as htrf, alpha and elisa, can only measure one or two parameters per sample, providing incomplewe informawion of WHE drxg¶v biological mechanisms. Furthermore, these technologies detect analytes or activity outside the cell, which can result in misleading hits as compounds may fail to achieve the same effect in cell samples. To accelerate drug discovery, there is a major need for multiplexed screening at high throughput. The long-term goal of this application is to develop high-throughput flow cytometry to enable multiplexed drug screening. Although flow cytometry can measure up to 30 parameters per sample at single-cell resolution, its throughput has thus far been severely limited by its slow sampling speed. The short-term goal of phase i is developing novel laser particle (LP) probes to barcode and pool cell samples to improve throughput, and demonstrate feasibility using a custom-built flow cytometer. This approach can eliminate sampling dead time, reducing acquisition time from 1 hour per 384-well plate to a few minutes. Furthermore, sample barcoding reduces reagent consumption and increases statistical robustness as samples are processed and stained together after pooling. Completion of phase i research will establish the feasibility of high-throughput flow cytometry using LP barcodes. Development of this technology will greatly benefit the drug discovery industry by saving time and cost.
Transactions
| Action | Federal | Non-federal | Date |
|---|---|---|---|
| Amendment | $0 | $0 | |
| Amendment | $0 | $0 | |
| Amendment | $0 | $0 | |
| Amendment | $0 | $0 | |
| Amendment | $0 | $0 | |
| Amendment | $253,256 | $0 | |
| Original award | $253,256 | $253,256 |
Quick answers
Who received award R43GM140527?
R43GM140527 was awarded to Lase Innovation Inc. by National Institute of General Medical Sciences (National Institutes of Health) on February 1, 2021.
How much is award R43GM140527 worth?
$759,768 has been obligated on R43GM140527: $506,512 in federal funding and $253,256 in non-federal funding.
On GovTribe
See this award's full record
- Every transaction and subaward
- The recipient's other awards and teaming partners
- Similar grant awards
- Ask GovTribe AI about this award