Project Grant R43GM126624

This Project Grant award of $194,375 from the National Institutes of Health (NIH) Office of Research Infrastructure Programs (CFDA 93.351) supports the University of Wisconsin-Madison in developing robust genome editing tools for generating floxed alleles and editing amino acid changes in the zebrafish model system. The key objectives are to establish a synthetic exon-mediated integration approach to reliably add LoxP sites to target genes, enabling conditional knockout of genes in specific cell...

The National Science Foundation's Division of Integrative Organismal Systems awarded $1,526,917 to Stanford University on September 15, 2025, under the Biological Sciences (CFDA 47.074) program to develop genome editing tools and genomic resources for poeciliid fishes (guppies, mollies, and swordtails). The project will establish immortalized cell lines from multiple species, optimize CRISPR-based DNA editing protocols for both in vitro and in vivo applications, and generate high-quality,...

The National Science Foundation's Biological Sciences program awarded a $1,200,000 project grant to the University of California, Los Angeles (UCLA) Office of Research Administration to discover and characterize small CRISPR genome editing systems for virus-based delivery into plants. This 3-year project aims to develop new tools to enhance plant breeding and create crops with higher yields, drought resistance, and pest/temperature tolerance. The work includes screening metagenomic data to...

This Project Grant from the National Science Foundation's Technology, Innovation, and Partnerships program totaling $100,000 will support research to develop next-generation clustered regularly interspaced short palindromic repeat (CRISPR) systems for curing inherited diseases. Specifically, the awardee, the University of California, Berkeley, will work to circumvent in vivo delivery challenges with CRISPR-Cas9 by developing miniature RNA-guided genome editors smaller than Cas9 that can be...

This Project Grant award from the National Science Foundation's Biological Sciences program (CFDA 47.074) provides $701,884 to The Regents of the University of Colorado to develop a functional genomics tool kit for the filamentous cyanobacterium Nostoc punctiforme. The key products and services to be delivered include construction of a random barcode transposon-site sequencing library, generation of CRISPR tools for gene deletion and cargo DNA integration, and implementation of proximity...

This $322,020 Project Grant award from the National Science Foundation's (NSF) Division of Integrative Organismal Systems will support collaborative research at the University of California, Davis (UC Davis) to develop improved methods for CRISPR/Cas9-based gene editing in maize. The key objectives are to generate maize lines expressing high levels of Cas9 nuclease and to engineer and optimize viral vectors to efficiently deliver guide RNAs for both somatic and heritable gene editing. This...

This three-year, $1.8 million project grant from the National Science Foundation Division of Integrative Organismal Systems aims to develop rapid transformation and gene editing techniques in maize through the Biological Sciences program (CFDA 47.074). The University of Missouri System will work to establish a system for precisely inserting transgenes and editing genes at predetermined sites in the genome of maize. Researchers will create "landing pads" on five chromosomes of a...

The National Science Foundation (NSF), under its Biological Sciences program (CFDA 47.074), awarded the University of Wisconsin System $621,502 on August 15, 2023 for a project titled "COLLABORATIVE RESEARCH: EDGE FGT: DEVELOPMENT OF A COMPREHENSIVE SELECTION LIBRARY TO RECONCILE CORE METABOLIC KNOWLEDGE GAPS". The project aims to develop tools and resources (algorithms, strains, and plasmids) to rapidly identify the functions of genes in non-model organisms, in order to better...

This Project Grant award from the National Institute of General Medical Sciences (NIGMS) Biomedical Research and Research Training program (CFDA 93.859) supports research to improve the efficiency of CRISPR gene editing in human pluripotent stem cells. The goal is to develop a novel carbon nanotube array technology that can efficiently transfer nucleic acids and proteins into these cells to enhance CRISPR-based gene editing and homology-directed recombination. The $275,045 award to Advanced Gene...

The National Science Foundation (NSF) Division of Integrative Organismal Systems awarded a $277,978 Project Grant under the Biological Sciences program (CFDA 47.074) to the University of California, Berkeley. The 3-year grant, beginning on Sep 1, 2023, aims to develop new tools and resources to enable more efficient gene editing in maize, the world's most produced crop. Specifically, the project will generate maize lines that constitutively express the Cas9 gene editing enzyme and engineer...