3D diagnosis of hirschsprung disease using open-top light-sheet microscopy
Ended Project Grant Awarded Last action
- Award ID
- R43DK133083
- Recipient
-
Alpenglow Biosciences, Inc. UEI DVN2AF9A1YV3
- Awarding agency
-
National Institute of Diabetes and Digestive and Kidney Diseases
National Institutes of Health, Department of Health and Human Services
- Obligated
- $505,038
Award facts
- Assistance listing
- 93.847 Diabetes, Digestive, and Kidney Diseases Extramural Research
- Completion date
- Originally
- Place of performance
- Seattle, WA 98195, USA
Award details come from USAspending.gov.
Description
- Abstract
- Hirschsprung disease is a congenital defect that affects an estimated 1:5000 liveborn infants. IT is characterized by absent ganglion cells (NEURONS) in the distal rectum and a variable length of contiguous proximal bowel. The diagnosis of hirschsprung disease relies upon subjective pathologist interpretation of up to 100 tissue sections from suction rectal biopsies to prove the absence of ganglion cells and the presence of hypertrophic nerves. Due to the labor-intensive and time-consuming nature of the current diagnostic process, there is a need to more efficiently and comprehensively diagnose hirschsprung disease. Our team has pioneered the use of 3D open-top light-sheet (OTLS) microscopy, which enables rapid, high-throughput imaging of large clinical samples. In combination with cutting-edge machine learning techniques, we hypothesize that 3D OTLS microscopy can provide more accurate and consistent assessment of ganglion cells and hypertrophic nerves in suction rectal biopsies from patients with suspected hirschsprung disease. We will test this hypothesis by developing a multiplex staining protocol and machine learning analysis pipeline, which will be piloted on 100 rectal biopsy specimens from fresh resection specimens.
Transactions
| Action | Obligated | Date |
|---|---|---|
| Amendment | -$6 | |
| Amendment | $252,522 | |
| Original award | $252,522 |
Quick answers
Who received award R43DK133083?
R43DK133083 was awarded to Alpenglow Biosciences, Inc. by National Institute of Diabetes and Digestive and Kidney Diseases (National Institutes of Health) on April 1, 2022.
How much is award R43DK133083 worth?
$505,038 has been obligated on R43DK133083.
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