Project Grant R43AI179513
- This National Science Foundation Project Grant of $180,493 will support the development of an ultrasensitive point-of-care diagnostic system using clustered regularly interspaced short palindromic repeats (CRISPR) technology for early disease detection without pre-amplification of viral genomes. Funded under the NSF's Office of Integrative Activities program (CFDA 47.083), which enhances STEM competitiveness through capacity building and broadening participation, this 24-month project will...
- This Project Grant award from the National Institute of General Medical Sciences (CFDA 93.859 - Biomedical Research and Research Training) to Kasa Bio, L.L.C., a woman-owned small business, aims to develop a higher-order multiplex digital PCR (dPCR) method for detecting important gene fusions related to chronic myeloid leukemia. The $345,285 award, with a performance period from May 1, 2025 to April 30, 2026, will fund the development of Kasa Bio's proprietary "ZIP-Melt" multiplex...
- This Project Grant award, totaling $354,570.00, was provided by the National Institute of Allergy and Infectious Diseases (NIAID) under the Allergy and Infectious Diseases Research program (CFDA 93.855). The award aims to develop a cost-effective and user-friendly point-of-care (POC) diagnostic tool, employing a novel "CamoChip" technology, for highly sensitive and multiplex testing of biomarkers related to HIV/HBV co-infection. The key products and services to be delivered under...
- This National Science Foundation (NSF) Technology, Innovation, and Partnerships (CFDA 47.084) Phase I Small Business Technology Transfer (STTR) project aims to develop a novel, cost-effective, and rapid CRISPR-Cas12a diagnostic platform to screen newborns for congenital cytomegalovirus (CCMV). CCMV affects approximately 25,000 U.S. babies annually, leading to hearing loss and neurological issues in 25% of infected children with a 0.5% mortality rate. The $305,000 award to Cleaved Diagnostic...
- This $199,997 federal Project Grant, awarded by the National Science Foundation's Engineering program (CFDA 47.041), aims to improve the stability and sensitivity of a heat-resistant version of the CRISPR-Cas13 enzyme for rapid and accurate detection of disease-causing RNA. The collaborative research project between William Marsh Rice University and its partners will leverage mechanism-based protein engineering and electrochemical devices to generate next-generation RNA detection tools for...
- This National Science Foundation (NSF) Small Business Innovation Research (SBIR) Phase I award to RT Microdx Inc. provides $274,362 to develop a novel molecular diagnostic platform for detecting respiratory diseases like strep throat. The platform aims to provide laboratory-quality accuracy for at-home use by non-healthcare professionals. Key technical objectives include optimizing the sensitivity of the platform's pH-sensitive polymer detection mechanism and ensuring robust performance at...
- The Department of Defense, through the Military Medical Research and Development program (CFDA 12.420), awarded a $1,857,470 Cooperative Agreement to The Geneva Foundation to develop a multiplex testing platform that can simultaneously detect and identify Plasmodium species infections. This platform will serve as both a diagnostic tool and an epidemiological surveillance tool. The project performance period is from June 1, 2024 to May 31, 2028. Under a $268,312 sub-award, Meso Scale...
- This $960,000 National Science Foundation project grant supports the development of a multiplexed plasmon-enhanced lateral flow assay for point-of-care detection of SARS-CoV-2 RNA and antigens. Funded under the NSF Engineering program (CFDA 47.041), the grantee, Washington University, will design, validate and build a novel diagnostic platform with sensitivity exceeding standard laboratory tests. Key deliverables include an ultrasensitive multiplexed lateral flow assay for simultaneous...
- The National Science Foundation awarded a $199,999 Project Grant under the Engineering (CFDA 47.041) program to The Trustees of the University of Pennsylvania to develop a next-generation RNA detection tool for infectious disease diagnosis. The project aims to engineer a thermostable version of the CRISPR-Cas13 enzyme to improve its stability and sensitivity for field applications. By combining the enhanced Cas13 enzyme with advanced electrochemical devices, the researchers will create an...
- This $402,222 National Science Foundation Project Grant, funded through the Engineering program (CFDA 47.041), supports the development of a new class of simple, inexpensive, portable, and rapid molecular diagnostic platforms. The awardee, University of Cincinnati, will engineer an instrument that can reliably and quickly detect infectious diseases in human samples using a combination of biochemistry, magnetism, fluid physics, and machine learning insights. Specifically, the university will...
AN INSTRUMENT-FREE MALARIA DIAGNOSTIC - ABSTRACT WE PROPOSE TO DEVELOP A SENSITIVE, RAPID, AND AFFORDABLE CRISPR-BASED DIAGNOSTIC TEST FOR MALARIA PARASITES. THE ASSAY WILL BE SIMPLE TO RUN, REQUIRE NO INSTRUMENTATION OR ADVANCED TRAINING, AND BE SUITABLE FOR RESOURCE-LIMITED REGIONS. IN THE PHASE I, WE WILL DESIGN AND OPTIMIZE OUR MULTI- CHAMBERED FAST-CRISPR DIAGNOSTIC USING CULTURED PARASITES AND PLASMID DNA TARGETS. LATER IN THE PHASE I OR IN FOLLOW-UP STUDIES, WE WILL PROVIDE DIAGNOSTIC KITS TO LABORATORIES IN ENDEMIC REGIONS FOR BETA TESTING. IN SPECIFIC AIM 1, WE WILL OPTIMIZE THE SELECTION OF OLIGOS USED IN ISOTHERMAL AMPLIFICATION OF TARGETED REGIONS AND OPTIMIZE THE LYSIS PROCESS TO EXPOSE THE DNA. BECAUSE REACTANTS FROM ONE STEP OF THE PROCESS FLOW INTO THE NEXT CHAMBER, WE WILL ENSURE THAT BUFFER COMPOSITIONS ARE COMPATIBLE. IN SPECIFIC AIM 2, WE WILL OPTIMIZE THE CRISPR/CAS12A REACTIONS FOR ACTIVATION OF THE ENDONUCLEASE ACTIVITY UPON SENSING THE TARGET SEQUENCES. DURING THIS AIM, WE WILL ASSESS THE USE OF MULTIPLE TARGETS AND DETERMINE ASSAY SPECIFICITY AND SENSITIVITY. IN SPECIFIC AIM 3, WE WILL DEVELOP THE CONFIGURATION OF THE FAST DEVICE AND PLAN FUTURE MANUFACTURING SCALE-UP.
Mod # | Description | ReasonForModification | Federal Obligation | Date |
|---|---|---|---|---|
| Not listed | $291.1k | 8/6/25 | ||
| Not listed | $297.8k | 5/30/24 |