Project Grant R43AI124933

Micropure Genomics Inc. received a $274,199 Project Grant award from the National Science Foundation to advance methods for rapid, end-to-end sample preparation for sequencing applications. Under the NSF Technology, Innovation, and Partnerships program (CFDA 47.084), the company will develop an automated process relying on electro-hydrodynamic trapping to isolate genomic material like DNA or RNA from cells in a flow cartridge. The process aims to significantly reduce preparation time while...

Triton Systems, Inc. received a $249,712 Project Grant award from the Department of Energy Office of Science on February 22, 2021 to develop a fast, scalable, automated, one-pot process for assembly of DNA oligonucleotides into large gene sequences. The work will be performed in Chelmsford, Massachusetts through February 21, 2022. The award is being made under the Office of Science Financial Assistance Program (CFDA #81.049), which supports basic research to deliver scientific discoveries and...

This Project Grant award from the National Institutes of Health's National Human Genome Research Institute provided $768,678 to Molecular Assemblies, Inc. to develop a fully enzymatic oligonucleotide synthesis cycle using engineered template-independent polymerases and a novel phosphate dNTP blocking group. The award supports the goals of the Human Genome Research program (CFDA 93.172) to accelerate genome research and its application to human health. Specifically, the awardee will engineer...

This Project Grant award, valued at $215,088, was provided by the National Human Genome Research Institute (NHGRI) under the Human Genome Research Federal Grant Program (CFDA 93.172). The grant supports the development of an "exhaustive de novo RNA sequencing method" by Directseq Biosciences, Inc., a small disadvantaged business based in Schenectady, New York. This innovative technology, referred to as Next-Generation Mass Spectrometry-based Sequencing (NGMS-SEQ), aims to...

This $256,000 National Science Foundation Engineering grant supports the development of an easy RNA adenylation method for deep sequencing of picogram RNA samples with high resolution. Specifically, Ezrabio Inc. will develop a versatile and cost-effective library construction technology that enables protein translation monitoring on a variety of biological samples. The proposed technology relies on a novel enzyme system capable of 5' adenylation and 3' polyadenylation of RNA fragments to...

This Project Grant award from the National Human Genome Research Institute (NHGRI), under the Human Genome Research program (CFDA 93.172), provides $314,538 to Dnali Data Technologies, Inc. to advance the commercial potential of a novel enzymatic technology for producing chemically modified synthetic DNA. The key objectives are: 1) creating modified DNA products that are not currently available from any existing vendor, 2) developing an innovation to eliminate the most costly reagent in the...

This $406,500 federal Project Grant from the National Human Genome Research Institute (NHGRI), under the Human Genome Research program (CFDA 93.172), aims to advance "Next-Generation Mass Spectrometry-based Sequencing (NGMS-SEQ)" methods for high-throughput, bias-free sequencing and quantitative mapping of RNA modifications. The funded research seeks to: 1) enhance NGMS-SEQ techniques to directly sequence tRNA samples, including physiologically relevant ones, and 2) improve...

This Project Grant award from the National Science Foundation (NSF) under the Technology, Innovation, and Partnerships (TIP) program (CFDA 47.084) supports the development of an autonomous, miniaturized system for DNA extraction and preparation for sequencing. The $275,000 award to Anyglabs, Inc. will fund the creation of a portable, automated device capable of high-throughput DNA processing in space and other austere environments. The goal is to democratize access to advanced molecular...

This Project Grant award from the National Institute of General Medical Sciences (NIGMS) under the Biomedical Research and Research Training Program (CFDA 93.859) provides $394,857 to the University of Nebraska to develop a system to protect and concentrate large DNA molecules for genome analysis. The project aims to create an inverted insert to protect DNA during cell lysis and a 3D-printed device with an acrylic roadblock to concentrate chromosomal DNA, enabling the analysis of structural...

The National Institute of General Medical Sciences (NIGMS) awarded a $305,892 Project Grant (CFDA 93.859 - Biomedical Research and Research Training) to Dotquant LLC, a for-profit biotechnology research and development company, to develop a new in-vitro transcription kit that can produce chemically modified, serum-stable RNA at high yields. The goal is to create a kit that combines the high efficiency and low cost of wild-type T7 RNA polymerase with the ability to incorporate modified...