100%
Skip to main content
Platform
GovTribe AI
AI-powered insights to accelerate your government contracting workflow.
MCP Server
Connect GovTribe to your AI tools.
Beacon
Find and connect with key government decision-makers.
Profiles
Federal Agencies
Federal Contract Vehicles
Federal Grant Programs
Major Defense Acquisition Programs
NAICS Categories
PSC Categories
States
Jurisdictions
NIGP Categories
UNSPSC Categories
Vendors
Enrichment By Clearbit
Reports
New Entrants
Funding Analysis
Vehicle Analysis
Groups
Capture
Pipelines
Pursuits
Alerts
Teaming
Data
Awards
Forecasts
Federal Opportunities
State & Local Opportunities
Files
Prime and Subcontractors
Activity
Search
Exports
Solutions
Use Case
Opportunity Identification
Capture Management
Competitive Intelligence
Teaming & Partner Identification
Proposal Management
Strategic Leadership
Go-to-Market Strategy
Industry
Federal Contractors
State & Local Contractors
Grant Seekers
Government Agencies
Research & Consulting
Pricing
More
About Us
Features
Get A Demo
Sign Up
Blog
User Guide
Data Model
GovTribe for Agents
Login
Contact sales
Try for free
Login
Contact sales
Try for free
All Federal Grant Awards
Project Grant R42ES023724
Award Date
8/1/14
Completion Date
3/31/20
Dollars Obligated
$1.4M
Overview
Activity
5
Transactions
5
Subawards
Similar Awards
Federal Agency
National Institute of Environmental Health Sciences
Awardee
Seatox Research Inc. (SP1EE9Z4GYB5)
Federal Grant Program
93.113
Assistance Type
Project Grant
Place of Performance
Wilmington, NC 28409, USA
Update #1
Update #2
DEVELOPMENT OF USER-FRIENDLY FLUORESCENCE BASED ASSAYS FOR MARINE TOXINS
Posted 3/22/17
5
1
Mod #
Description
ReasonForModification
Federal Obligation
Date
Not listed
DEVELOPMENT OF USER-FRIENDLY FLUORESCENCE BASED ASSAYS FOR MARINE TOXINS - ABSTRACT HARMFUL ALGAL BLOOMS (HABS) ARE BECOMING FREQUENT OCCURRENCES OFF THE COASTS OF THE UNITED STATES, WITH SPECIFIC INSTANCES IN THE COASTAL WATERS OF THE WEST COAST, THE GULF OF MEXICO, AND THE PACIFIC OCEAN. HISTORICALLY, HABS HAVE BEEN ASSOCIATED WITH FISH KILLS AND MARINE MAMMAL MORTALITIES; HOWEVER, THEIR EFFECTS ON HUMAN HEALTH AND ECONOMIC LOSS DUE TO HAB CONTAMINATION OF SEAFOOD ARE BECOMING MORE PREVALENT, WITH COST ESTIMATES AROUND $82 MILLION ANNUALLY. THE TOXIC EFFECTS OF HABS ARE PRECIPITATED BY THE PRODUCTION OF TOXINS BY VARIOUS SPECIES OF MARINE ALGAE AND MICROORGANISMS, WHICH ARE THEN CONSUMED BY FILTER FEEDING SHELLFISH AND FINFISH. ACCUMULATION IN SIGNIFICANT QUANTITIES CAN RESULT IN TOXICITY AND DEATH IN MARINE ANIMALS AND HUMANS. MANY OF THE NATURAL TOXINS PRODUCE BY MARINE PHYTOPLANKTON ARE HEAT AND ACID STABLE; THEREFORE, COOKING CONTAMINATED SEAFOOD DOES NOT ELIMINATE THE RISK OF POISONING. CURRENTLY, US STATE AGENCIES MONITOR FOR THE PRESENCE OF TOXIC PHYTOPLANKTON AND WHEN THE CELL COUNT REACHES A SET LEVEL, SHELLFISH BEDS AND FINFISH ARE TESTED FOR THE PRESENCE OF TOXIN. WHEN TOXIN LEVELS REACHES FDA SET LIMITS, FISHERY RESOURCES ARE CLOSED. HOWEVER, CURRENT METHODS FOR DETECTION OF MARINE TOXINS MOST COMMONLY ASSOCIATED WITH SEAFOOD POISONINGS IN THE US HAVE SERIOUS DRAWBACKS, INCLUDING LENGTHY ASSAY TIME, HIGH COST, ANIMAL USAGE, LOW SENSITIVITY AND/OR SAMPLE THROUGHPUT, OR SMALL WORKING RANGES. WE HAVE DEVELOPED FLUORESCENCE BASED RECEPTOR BINDING ASSAYS (FBAS) FOR THE TOXINS THAT CAUSE CIGUATERA (CIGUATOXINS), NEUROTOXIC SHELLFISH POISONING (BREVETOXINS), AND AMNESIC SHELLFISH POISONING (DOMOIC ACID). THESE ASSAYS WILL BE USED AS A RAPID TEST ALTERNATIVE TO CURRENT METHODS, AS THEY HAVE THE ADVANTAGES OF LOWER COST, HIGH SENSITIVITY AND LOWER ANIMAL USAGE. THE FBAS ARE AN IMPROVEMENT OVER THE RADIOLIGAND RECEPTOR BINDING ASSAYS (RBAS) FOR THESE TOXINS, AS THE FLUORESCENCE PLATFORM DOES NOT REQUIRE THE USE OF RADIOACTIVITY AND IS THUS SAFER AND FAR LESS EXPENSIVE. IN OUR PHASE I STUDIES, WE DEVELOPED THE PROOF-OF- CONCEPT FBAS BY CONJUGATING FLUOROPHORES TO THE TOXINS, SHOWING THE FLUORESCENT TOXIN CONJUGATES RETAIN BINDING TO THE RECEPTORS, AND DEMONSTRATING THE FLUORESCENT TOXIN CONJUGATES CAN DETECT THE PRESENCE OF STANDARD DILUTION CURVES (PURE SAMPLES OF TOXINS). IN THE PROPOSED PHASE II STUDIES, WE WILL EXPAND OUR PROOF- OF-CONCEPT FBAS TO ACCOMPLISH THE FOLLOWING GOALS: FIRST, WE WILL OPTIMIZE THE CURRENT FBA PLATFORM TO SOLVE TWO PAIN POINTS: THE NEED TO KEEP COMPONENTS FROZEN AND THE USE OF ANIMAL PARTS. WE WILL PRESERVE THE RECEPTOR COMPONENTS TO ALLOW FOR STABLE STORAGE AT ROOM TEMPERATURE. SHIPPING TO OUR PROTOTYPE CUSTOMERS, MANY OF WHOM ARE OVERSEAS, IS EXPENSIVE AND RISKY BECAUSE OF THE NEED TO KEEP COMPONENTS AT -80C. WE WILL ALSO CREATE A NEW RECEPTOR PREPARATION DERIVED FROM HUMAN CELL LINES, WHICH WILL BE RENEWABLE AND ELIMINATE THE NEED FOR RAT BRAINS, THUS FOLLOWING A SOCIETAL AND SCIENTIFIC TREND, AS WELL AS NIEHS IMPERATIVE, TO REDUCE RELIANCE ON ANIMALS FOR TOXICITY TESTING. SECONDLY, WE WILL FULLY VALIDATE OUR TEST KITS FOR SEAFOOD SAMPLES (WHICH ARE "DIRTIER" THAN PURE TOXIN SAMPLES) IN COMPARISON TO COMPETITOR METHODS (E.G. ELISAS, HPLC, AND LC/MS) FOR USE AS A COMMERCIAL RESEARCH TOOL. AS RBAS HAVE BEEN USED FOR A VARIETY OF SAMPLE MATRICES AND HAVE BEEN SHOWN TO STRONGLY CORRELATE WITH THE MOUSE BIOASSAY AND HPLC RESULTS IN SAMPLES, FBAS ARE BE THE NEXT PROGRESSIVE STEP IN THE DETECTION OF TOXINS IN SEAFOOD OR COASTAL WATER SAMPLES THEREBY PROTECTING HUMAN HEALTH AND AIDING IN THE MONITORING OF FISHERY RESOURCES.
($8k)
4/20/21
Not listed
DEVELOPMENT OF USER-FRIENDLY FLUORESCENCE BASED ASSAYS FOR MARINE TOXINS - ABSTRACT HARMFUL ALGAL BLOOMS (HABS) ARE BECOMING FREQUENT OCCURRENCES OFF THE COASTS OF THE UNITED STATES, WITH SPECIFIC INSTANCES IN THE COASTAL WATERS OF THE WEST COAST, THE GULF OF MEXICO, AND THE PACIFIC OCEAN. HISTORICALLY, HABS HAVE BEEN ASSOCIATED WITH FISH KILLS AND MARINE MAMMAL MORTALITIES; HOWEVER, THEIR EFFECTS ON HUMAN HEALTH AND ECONOMIC LOSS DUE TO HAB CONTAMINATION OF SEAFOOD ARE BECOMING MORE PREVALENT, WITH COST ESTIMATES AROUND $82 MILLION ANNUALLY. THE TOXIC EFFECTS OF HABS ARE PRECIPITATED BY THE PRODUCTION OF TOXINS BY VARIOUS SPECIES OF MARINE ALGAE AND MICROORGANISMS, WHICH ARE THEN CONSUMED BY FILTER FEEDING SHELLFISH AND FINFISH. ACCUMULATION IN SIGNIFICANT QUANTITIES CAN RESULT IN TOXICITY AND DEATH IN MARINE ANIMALS AND HUMANS. MANY OF THE NATURAL TOXINS PRODUCE BY MARINE PHYTOPLANKTON ARE HEAT AND ACID STABLE; THEREFORE, COOKING CONTAMINATED SEAFOOD DOES NOT ELIMINATE THE RISK OF POISONING. CURRENTLY, US STATE AGENCIES MONITOR FOR THE PRESENCE OF TOXIC PHYTOPLANKTON AND WHEN THE CELL COUNT REACHES A SET LEVEL, SHELLFISH BEDS AND FINFISH ARE TESTED FOR THE PRESENCE OF TOXIN. WHEN TOXIN LEVELS REACHES FDA SET LIMITS, FISHERY RESOURCES ARE CLOSED. HOWEVER, CURRENT METHODS FOR DETECTION OF MARINE TOXINS MOST COMMONLY ASSOCIATED WITH SEAFOOD POISONINGS IN THE US HAVE SERIOUS DRAWBACKS, INCLUDING LENGTHY ASSAY TIME, HIGH COST, ANIMAL USAGE, LOW SENSITIVITY AND/OR SAMPLE THROUGHPUT, OR SMALL WORKING RANGES. WE HAVE DEVELOPED FLUORESCENCE BASED RECEPTOR BINDING ASSAYS (FBAS) FOR THE TOXINS THAT CAUSE CIGUATERA (CIGUATOXINS), NEUROTOXIC SHELLFISH POISONING (BREVETOXINS), AND AMNESIC SHELLFISH POISONING (DOMOIC ACID). THESE ASSAYS WILL BE USED AS A RAPID TEST ALTERNATIVE TO CURRENT METHODS, AS THEY HAVE THE ADVANTAGES OF LOWER COST, HIGH SENSITIVITY AND LOWER ANIMAL USAGE. THE FBAS ARE AN IMPROVEMENT OVER THE RADIOLIGAND RECEPTOR BINDING ASSAYS (RBAS) FOR THESE TOXINS, AS THE FLUORESCENCE PLATFORM DOES NOT REQUIRE THE USE OF RADIOACTIVITY AND IS THUS SAFER AND FAR LESS EXPENSIVE. IN OUR PHASE I STUDIES, WE DEVELOPED THE PROOF-OF- CONCEPT FBAS BY CONJUGATING FLUOROPHORES TO THE TOXINS, SHOWING THE FLUORESCENT TOXIN CONJUGATES RETAIN BINDING TO THE RECEPTORS, AND DEMONSTRATING THE FLUORESCENT TOXIN CONJUGATES CAN DETECT THE PRESENCE OF STANDARD DILUTION CURVES (PURE SAMPLES OF TOXINS). IN THE PROPOSED PHASE II STUDIES, WE WILL EXPAND OUR PROOF- OF-CONCEPT FBAS TO ACCOMPLISH THE FOLLOWING GOALS: FIRST, WE WILL OPTIMIZE THE CURRENT FBA PLATFORM TO SOLVE TWO PAIN POINTS: THE NEED TO KEEP COMPONENTS FROZEN AND THE USE OF ANIMAL PARTS. WE WILL PRESERVE THE RECEPTOR COMPONENTS TO ALLOW FOR STABLE STORAGE AT ROOM TEMPERATURE. SHIPPING TO OUR PROTOTYPE CUSTOMERS, MANY OF WHOM ARE OVERSEAS, IS EXPENSIVE AND RISKY BECAUSE OF THE NEED TO KEEP COMPONENTS AT -80C. WE WILL ALSO CREATE A NEW RECEPTOR PREPARATION DERIVED FROM HUMAN CELL LINES, WHICH WILL BE RENEWABLE AND ELIMINATE THE NEED FOR RAT BRAINS, THUS FOLLOWING A SOCIETAL AND SCIENTIFIC TREND, AS WELL AS NIEHS IMPERATIVE, TO REDUCE RELIANCE ON ANIMALS FOR TOXICITY TESTING. SECONDLY, WE WILL FULLY VALIDATE OUR TEST KITS FOR SEAFOOD SAMPLES (WHICH ARE "DIRTIER" THAN PURE TOXIN SAMPLES) IN COMPARISON TO COMPETITOR METHODS (E.G. ELISAS, HPLC, AND LC/MS) FOR USE AS A COMMERCIAL RESEARCH TOOL. AS RBAS HAVE BEEN USED FOR A VARIETY OF SAMPLE MATRICES AND HAVE BEEN SHOWN TO STRONGLY CORRELATE WITH THE MOUSE BIOASSAY AND HPLC RESULTS IN SAMPLES, FBAS ARE BE THE NEXT PROGRESSIVE STEP IN THE DETECTION OF TOXINS IN SEAFOOD OR COASTAL WATER SAMPLES THEREBY PROTECTING HUMAN HEALTH AND AIDING IN THE MONITORING OF FISHERY RESOURCES.
($8k)
4/20/21
Not listed
DEVELOPMENT OF USER-FRIENDLY FLUORESCENCE BASED ASSAYS FOR MARINE TOXINS
$0
2/1/19
Not listed
DEVELOPMENT OF USER-FRIENDLY FLUORESCENCE BASED ASSAYS FOR MARINE TOXINS
$613.4k
3/6/18
Not listed
DEVELOPMENT OF USER-FRIENDLY FLUORESCENCE BASED ASSAYS FOR MARINE TOXINS
$854.5k
3/22/17