Capturing large-scale locus coeruleus single neuron activity in behaving rats with nanoelectronic threads (nets), an ultra-flexible multi-electrode probe
Ended Project Grant Awarded Last action
- Award ID
- R34NS123876
- Recipient
-
Helsingin Yliopisto UEI YM1UNKPWTJL9
- Awarding agency
-
National Institute of Neurological Disorders and Stroke
National Institutes of Health, Department of Health and Human Services
- Obligated
- $1,386,072
Award facts
- Assistance listing
- 93.173 Research Related to Deafness and Communication Disorders
- Completion date
- Originally
- Funding
-
- $1,115,979.00 Federal
- $270,093.00 Non-federal
- Place of performance
- Finland
Award details come from USAspending.gov.
Description
- Abstract
- Systems neuroscience has viewed the brainstem noradrenergic nucleus, locus coeruleus (LC), as the source of a global arousal signal, which modulates cognitive functions by altering operations throughout the entire central nervous system. This is achieved, presumably, by this small collection of only ~1,600 neurons in rodents spiking synchronously. Recently, however, the first large-scale recordings of many LC single units simultaneously in anesthetized rats demonstrated that LC population activity consists of a nuanced pattern of distinct ensembles that are independently active. In parallel, chemogenetic and optogenetic manipulations of the LC in behaving rodents revealed that the LC contains subsets of neurons that provide forebrain circuit-specific neuromodulation of specific cognitive functions. These recent advances compel the field to record LC ensembles in behaving animals. However, this requires surmounting the obstacle of brainstem movement in behaving animals which has heretofore limited LC recordings to multi-unit activity or one single unit. Here, we will establish the feasibility of ultra-flexible multi-electrode probes, called 'nanoelectronic threads' (nets), for months-long LC ensemble recordings in unanesthetized rats. As a proof-of-concept study demonstrating the feasibility of nets, we recorded ~150 single units simultaneously in the visual cortex of freely moving rats for up to four months. In this project, we aim to implant 512 electrodes in the LC of the head-fixed behaving RAT and record 100S of LC single units simultaneously, along with ongoing arousal/behavioral state (pupil size, locomotion, orofacial movements, etc.). By increasing the number of units recorded by orders of magnitude, this project will be transformational for LC research. Our approach will be broadly useful to neuroscientists recording in other brainstem and subcortical structures in which recording many single units is difficult. Our overall goal is to use this R34 to establish the method, which will enable a subsequent targeted brain circuits project R01 submission focused on the role of LC ensemble activity in the circuit basis of cognitive rigidity that is precipitated by chronic, months-long stress.
Transactions
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| Original award | $270,093 | $270,093 |
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Quick answers
Who received award R34NS123876?
R34NS123876 was awarded to Helsingin Yliopisto by National Institute of Neurological Disorders and Stroke (National Institutes of Health) on August 15, 2021.
How much is award R34NS123876 worth?
$1,386,072 has been obligated on R34NS123876: $1,115,979 in federal funding and $270,093 in non-federal funding.
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