Investigating spatial and temporal heterogeneity in mitochondria during aging using in vivo high-content chemical imaging

Ended Project Grant Awarded Last action

Award ID
R21AG086974
Recipient
Awarding agency
Obligated
$935,708

Award facts

Assistance listing
93.866 Aging Research
Completion date
Place of performance
Atlanta, GA 30318, USA

Award details come from USAspending.gov.

Description

As the agency reported it.

Abstract
Title: investigating spatial and temporal heterogeneity in mitochondria during aging using in vivo high- content chemical imaging the spatial and temporal regulation of mitochondria is critical for controlling various cellular processes, including energy balance and metabolism, in specific locations and timeframes. Mitochondria have the ability to dynami- cally adjust their shape, position, activity, and interactions with other cellular components, such as lipid droplets (LDS), to fulfill the diverse functional requirements throughout an animal's lifespan. However, the precise relation- ship between these spatial and temporal changes in mitochondria and the physiological homeostasis of host cells and mitochondria is not fully understood. To address this question, we have developed advanced in vivo chemi- CAL imaging techniques, specifically broadband coherent anti-stokes raman scattering (BCARS) and two-photon fluorescence lifetime imaging (2P-FLIM), and a genetically-tractable platform utilizing the nematode caenorhab- ditis elegans as the model organism. While BCARS and 2P-FLIM provide organelle-resolved data with very high information content associated with the metabolic states of subcellular compartments, the established in vivo platform offers the opportunity to examine various conditions. Our research aims to unravel the heterogeneity of mitochondria in different physiological states, including their interactions with LDS, subcellular localization, and morphology in various tissues and cell types, and under different age and disease conditions within intact living animals. The successful completion of our proposed studies is expected to significantly advance our understand- ING of the intricate relationship between physiological homeostasis and mitochondrial dynamics. Furthermore, the establishment of these novel imaging and analysis approaches will revolutionize the field of mitochondrial biology by providing a groundbreaking tool for reliably examining mitochondrial heterogeneity in vivo.

Transactions

Action Obligated Date
Amendment $18,217
Amendment $163,958
Amendment $163,958
Amendment $196,525
Amendment $196,525
Original award $196,525

Subawards

Subrecipient Amount Awarded
University Of Florida To investigate how spatial and temporal heterogeneity of mitochondria impact physiological states throughout animal lifespan. We will utilize advanced in vivo… $134,200

Quick answers

Who received award R21AG086974?

R21AG086974 was awarded to Georgia TECH Research Corp by National Institute on Aging (National Institutes of Health) on August 1, 2024.

How much is award R21AG086974 worth?

$935,708 has been obligated on R21AG086974.

On GovTribe

See this award's full record

  • Every transaction and subaward
  • The recipient's other awards and teaming partners
  • Similar grant awards
  • Ask GovTribe AI about this award
Try for free Login