This Project Grant award, totaling $351,294.00, was provided by the National Institute of General Medical Sciences (NIGMS) under the Biomedical Research and Research Training program (CFDA 93.859). The award will support research by Dickinson College to develop orthogonal split luciferases for imaging multiplexed cellular behaviors. The key objectives are to engineer unique split luciferase binding pairs using protein engineering and directed evolution techniques, and then test the optimized probes in mammalian cells, tissue models, and live mice. This work aims to expand the utility of bioluminescence imaging by enabling the simultaneous tracking of multiple cell types. The anticipated outcomes include new bioluminescent protein tools that can be leveraged by the broader research community to study complex disease states, host-pathogen interactions, and cancer-immune cell dynamics. The award period is from Sep 26, 2023 to Aug 31, 2026.
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